LOCUS Exported 10768 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).. ACCESSION . VERSION . KEYWORDS BE3(E63Q)-P2A-EGFP (pRZ189) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10768) AUTHORS Grunewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK TITLE Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. JOURNAL Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. PUBMED 30995674 REFERENCE 2 (bases 1 to 10768) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10768 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(87..106) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 152..207 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(206..225) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(568..584) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(568..584) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(581..603) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 592..608 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(616..646) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 661..682 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 741..936 /label=SV40 promoter /note="SV40 early promoter" rep_origin 787..922 /label=SV40 ori /note="SV40 origin of replication" primer_bind 849..868 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 942..1076 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(979..998) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1033..1052 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1144..1161) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1315..1903) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1395..1414) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2074..2934) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2697..2716 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2935..3039) /gene="bla" /label=AmpR promoter enhancer 3070..3449 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3451..3728 /label=chicken beta-actin promoter intron 3729..4745 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 4669..4691 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 4753..4772 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 5579..9679 /codon_start=1 /product="nickase mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9(D10A) /note="generates RNA-guided single strand nicks in DNA due to the D10A mutation in the RuvC catalytic domain" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 9692..9940 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 9953..9973 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 9992..10048 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 10049..10765 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(10094..10115) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(10355..10374) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 10702..10723 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat 61 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc 121 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata 181 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta 241 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc 301 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat 361 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg 421 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc 481 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac 541 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 601 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 661 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 721 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact 781 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 841 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 901 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt 961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 1021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct 1081 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 1141 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 1201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 1261 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 1321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 1381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 1441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 1501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 1561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 1621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 1681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1741 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1921 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 1981 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 2041 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 2101 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 2161 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 2221 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 2281 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 2341 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 2401 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2461 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2521 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2581 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2641 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2701 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2761 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 2821 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 2881 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 2941 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 3001 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 3061 acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 3121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 3181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 3241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 3301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 3361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 3421 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc 3481 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt 3541 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg 3601 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc 3661 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc 3721 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc 3781 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc 3841 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc 3901 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg 3961 gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct 4021 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg 4081 cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc 4141 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc 4201 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta 4261 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg 4321 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga 4381 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg 4441 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc 4501 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg 4561 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg 4621 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct 4681 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc 4741 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattct 4801 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgagct cagagactgg 4861 cccagtggct gtggacccca cattgagacg gcggatcgag ccccatgagt ttgaggtatt 4921 cttcgatccg agagagctcc gcaaggagac ctgcctgctt tacgaaatta attggggggg 4981 ccggcactcc atttggcgac atacatcaca gaacactaac aagcacgtcc aagtcaactt 5041 catcgagaag ttcacgacag aaagatattt ctgtccgaac acaaggtgca gcattacctg 5101 gtttctcagc tggagcccat gcggcgaatg tagtagggcc atcactgaat tcctgtcaag 5161 gtatccccac gtcactctgt ttatttacat cgcaaggctg taccaccacg ctgacccccg 5221 caatcgacaa ggcctgcggg atttgatctc ttcaggtgtg actatccaaa ttatgactga 5281 gcaggagtca ggatactgct ggagaaactt tgtgaattat agcccgagta atgaagccca 5341 ctggcctagg tatccccatc tgtgggtacg actgtacgtt cttgaactgt actgcatcat 5401 actgggcctg cctccttgtc tcaacattct gagaaggaag cagccacagc tgacattctt 5461 taccatcgct cttcagtctt gtcattacca gcgactgccc ccacacattc tctgggccac 5521 cgggttgaaa agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagtga 5581 taaaaagtat tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac 5641 cgatgaatac aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc 5701 gattaaaaag aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac 5761 tcgcctgaaa cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt 5821 acaagaaatt tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga 5881 agagtccttc cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat 5941 agtagatgag gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct 6001 agttgactca actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat 6061 aaagttccgt gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga 6121 caaactgttc atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa 6181 tgcaagtggc gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct 6241 agaaaacctg atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat 6301 agcgctctca ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc 6361 caaattgcag cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat 6421 tggagatcag tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct 6481 atctgacata ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat 6541 caaaaggtac gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca 6601 actgcctgag aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta 6661 tattgacggc ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa 6721 gatggatggg acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca 6781 gcggactttc gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat 6841 acttagaagg caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa 6901 aatcctaacc tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt 6961 cgcatggatg acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt 7021 cgataaaggt gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt 7081 accgaacgaa aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa 7141 tgaactcacg aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg 7201 agaacagaag aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa 7261 gcaattgaaa gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg 7321 ggtagaagat cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa 7381 agataaggac ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac 7441 tcttaccctc tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct 7501 gttcgacgat aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt 7561 gtcgcggaaa cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt 7621 tctaaagagc gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt 7681 aaccttcaaa gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga 7741 acatattgcg aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa 7801 agtagtggat gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga 7861 gatggcacgc gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa 7921 gagaatagaa gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga 7981 aaatacccaa ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat 8041 gtatgttgat caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt 8101 accccaatcc tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa 8161 gaaccgaggg aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta 8221 ttggcggcag ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa 8281 agctgagagg ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt 8341 ggaaacccgc caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa 8401 atacgacgag aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt 8461 ggtgtcggac ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca 8521 ccatgcgcac gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc 8581 gaagctagaa agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat 8641 cgcgaaaagc gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat 8701 tatgaatttc tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt 8761 aattgaaacc aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac 8821 ggtgagaaaa gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac 8881 cggagggttt tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg 8941 taaaaaggac tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc 9001 tgtcctagta gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga 9061 attattgggg ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct 9121 tgaggcgaaa ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag 9181 tctgtttgag ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa 9241 ggggaacgaa ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta 9301 cgagaagttg aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca 9361 caaacattat ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct 9421 agctgatgcc aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat 9481 acgtgagcag gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc 9541 cgcattcaag tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt 9601 gctagacgcg acactgattc accaatccat cacgggatta tatgaaactc ggatagattt 9661 gtcacagctt gggggtgact ctggtggttc tactaatctg tcagatatta ttgaaaagga 9721 gaccggtaag caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga 9781 agtcattggg aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac 9841 cgacgagaat gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt 9901 catacaggat agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa 9961 gaagaggaaa gtcggaggcg gtggaagcgg cgcaacaaac ttctctctgc tgaaacaagc 10021 cggagatgtc gaagagaatc ctggaccgat ggtgagcaag ggcgaggagc tgttcaccgg 10081 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 10141 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 10201 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 10261 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 10321 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 10381 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 10441 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 10501 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 10561 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 10621 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 10681 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 10741 tctcggcatg gacgagctgt acaagtga //