LOCUS Exported 13254 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression of human flag-tagged mTor. ACCESSION . VERSION . KEYWORDS pcDNA3-Flag mTOR wt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13254) AUTHORS Vilella-Bach M, Nuzzi P, Fang Y, Chen J TITLE The FKBP12-rapamycin-binding domain is required for FKBP12-rapamycin-associated protein kinase activity and G1 progression. JOURNAL J Biol Chem. 1999 Feb 12. 274(7):4266-72. PUBMED 9933627 REFERENCE 2 (bases 1 to 13254) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13254 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(46..65) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 237..616 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 617..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 771..791 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 865..884 /label=T7 /note="T7 promoter, forward primer" promoter 865..883 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 969..978 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3850..3859 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7059..7337 /codon_start=1 /product="FKBP-rapamycin binding domain of human FRAP" /label=FRB /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK" CDS 7071..7343 /codon_start=1 /product="FKBP-rapamycin binding domain of human FRAP" /label=FRB /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL" primer_bind complement(8758..8774) /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8758..8774) /label=pBluescriptSK /note="For pBluescript vector" promoter complement(8809..8827) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(8810..8827) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(8847..8864) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8853..9077 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9123..9551 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9210..9229) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9420..9441 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9560..9580) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9565..9894 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9745..9880 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9807..9826 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9961..10755 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(10015..10034) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 10625..10644 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 10929..11050 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10966..10985) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11020..11039 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(11099..11115) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11099..11115) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11112..11134) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11123..11139 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11147..11177) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11192..11213 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(11330..11347) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11501..12089) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11581..11600) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(12260..13120) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12883..12902 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(13121..13225) /gene="bla" /label=AmpR promoter ORIGIN 1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc 841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta 901 ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattctgcag atatccatca 961 cactggcggc cgccatggat tataaagatg atgatgatat gcttggaacc ggacctgccg 1021 ccgccaccac cgctgccacc acatctagca atgtgagcgt cctgcagcag tttgccagtg 1081 gcctaaagag ccggaatgag gaaaccaggg ccaaagccgc caaggagctc cagcactatg 1141 tcaccatgga actccgagag atgagtcaag aggagtctac tcgcttctat gaccaactga 1201 accatcacat ttttgaattg gtttccagct cagatgccaa tgagaggaaa ggtggcatct 1261 tggccatagc tagcctcata ggagtggaag gtgggaatgc cacccgaatt ggcagatttg 1321 ccaactatct tcggaacctc ctcccctcca atgacccagt tgtcatggaa atggcatcca 1381 aggccattgg ccgtcttgcc atggcagggg acacttttac cgctgagtac gtggaatttg 1441 aggtgaagcg agccctggaa tggctgggtg ctgaccgcaa tgagggccgg agacatgcag 1501 ctgtcctggt tctccgtgag ctggccatca gcgtccctac cttcttcttc cagcaagtgc 1561 aacccttctt tgacaacatt tttgtggccg tgtgggaccc caaacaggcc atccgtgagg 1621 gagctgtagc cgcccttcgt gcctgtctga ttctcacaac ccagcgtgag ccgaaggaga 1681 tgcagaagcc tcagtggtac aggcacacat ttgaagaagc agagaaggga tttgatgaga 1741 ccttggccaa agagaagggc atgaatcggg atgatcggat ccatggagcc ttgttgatcc 1801 ttaacgagct ggtccgaatc agcagcatgg agggagagcg tctgagagaa gaaatggaag 1861 aaatcacaca gcagcagctg gtacacgaca agtactgcaa agatctcatg ggcttcggaa 1921 caaaacctcg tcacattacc cccttcacca gtttccaggc tgtacagccc cagcagtcaa 1981 atgccttggt ggggctgctg gggtacagct ctcaccaagg cctcatggga tttgggacct 2041 cccccagtcc agctaagtcc accctggtgg agagccggtg ttgcagagac ttgatggagg 2101 agaaatttga tcaggtgtgc cagtgggtgc tgaaatgcag gaatagcaag aactcgctga 2161 tccaaatgac aatccttaat ttgttgcccc gcttggctgc attccgacct tctgccttca 2221 cagataccca gtatctccaa gataccatga accatgtcct aagctgtgtc aagaaggaga 2281 aggaacgtac agcggccttc caagccctgg ggctactttc tgtggctgtg aggtctgagt 2341 ttaaggtcta tttgcctcgc gtgctggaca tcatccgagc ggccctgccc ccaaaggact 2401 tcgcccataa gaggcagaag gcaatgcagg tggacgccac agtcttcact tgcatcagca 2461 tgctggctcg agcaatgggg ccaggcatcc agcaggatat caaggagctg ctggagccca 2521 tgctggcagt gggactaagc cctgccctca ctgcagtgct ctacgacctg agccgtcaga 2581 ttccacagct aaagaaggac attcaagatg ggctactgaa aatgctgtcc ctggtcctta 2641 tgcacaaacc ccttcgccac ccaggcatgc ccaagggcct ggcccatcag ctggcctctc 2701 ctggcctcac gaccctccct gaggccagcg atgtgggcag catcactctt gccctccgaa 2761 cgcttggcag ctttgaattt gaaggccact ctctgaccca atttgttcgc cactgtgcgg 2821 atcatttcct gaacagtgag cacaaggaga tccgcatgga ggctgcccgc acctgctccc 2881 gcctgctcac accctccatc cacctcatca gtggccatgc tcatgtggtt agccagaccg 2941 cagtgcaagt ggtggcagat gtgcttagca aactgctcgt agttgggata acagatcctg 3001 accctgacat tcgctactgt gtcttggcgt ccctggacga gcgctttgat gcacacctgg 3061 cccaggcgga gaacttgcag gccttgtttg tggctctgaa tgaccaggtg tttgagatcc 3121 gggagctggc catctgcact gtgggccgac tcagtagcat gaaccctgcc tttgtcatgc 3181 ctttcctgcg caagatgctc atccagattt tgacagagtt ggagcacagt gggattggaa 3241 gaatcaaaga gcagagtgcc cgcatgctgg ggcacctggt ctccaatgcc ccccgactca 3301 tccgccccta catggagcct attctgaagg cattaatttt gaaactgaaa gatccagacc 3361 ctgatccaaa cccaggtgtg atcaataatg tcctggcaac aataggagaa ttggcacagg 3421 ttagtggcct ggaaatgagg aaatgggttg atgaactttt tattatcatc atggacatgc 3481 tccaggattc ctctttgttg gccaaaaggc aggtggctct gtggaccctg ggacagttgg 3541 tggccagcac tggctatgta gtagagccct acaggaagta ccctactttg cttgaggtgc 3601 tactgaattt tctgaagact gagcagaacc agggtacacg cagagaggcc atccgtgtgt 3661 tagggctttt aggggctttg gatccttaca agcacaaagt gaacattggc atgatagacc 3721 agtcccggga tgcctctgct gtcagcctgt cagaatccaa gtcaagtcag gattcctctg 3781 actatagcac tagtgaaatg ctggtcaaca tgggaaactt gcctctggat gagttctacc 3841 cagctgtgtc catggtggcc ctgatgcgga tcttccgaga ccagtcactc tctcatcatc 3901 acaccatggt tgtccaggcc atcaccttca tcttcaagtc cctgggactc aaatgtgtgc 3961 agttcctgcc ccaggtcatg cccacgttcc ttaatgtcat tcgagtctgt gatggggcca 4021 tccgggaatt tttgttccag cagctgggaa tgttggtgtc ctttgtgaag agccacatca 4081 gaccttatat ggatgaaata gtcaccctca tgagagaatt ctgggtcatg aacacctcaa 4141 ttcagagcac gatcattctt ctcattgagc aaattgtggt agctcttggg ggtgaattta 4201 agctctacct gccccagctg atcccacaca tgctgcgtgt cttcatgcat gacaacagcc 4261 caggccgcat tgtctctatc aagttactgg ctgcaatcca gctgtttggc gccaacctgg 4321 atgactacct gcatttactg ctgcctccta ttgttaagtt gtttgatgcc cctgaagctc 4381 cactgccatc tcgaaaggca gcgctagaga ctgtggaccg cctgacggag tccctggatt 4441 tcactgacta tgcctcccgg atcattcacc ctattgttcg aacactggac cagagcccag 4501 aactgcgctc cacagccatg gacacgctgt cttcacttgt ttttcagctg gggaagaagt 4561 accaaatttt cattccaatg gtgaataaag ttctggtgcg acaccgaatc aatcatcagc 4621 gctatgatgt gctcatctgc agaattgtca agggatacac acttgctgat gaagaggagg 4681 atcctttgat ttaccagcat cggatgctta ggagtggcca aggggatgca ttggctagtg 4741 gaccagtgga aacaggaccc atgaagaaac tgcacgtcag caccatcaac ctccaaaagg 4801 cctggggcgc tgccaggagg gtctccaaag atgactggct ggaatggctg agacggctga 4861 gcctggagct gctgaaggac tcatcatcgc cctccctgcg ctcctgctgg gccctggcac 4921 aggcctacaa cccgatggcc agggatctct tcaatgctgc atttgtgtcc tgctggtctg 4981 aactgaatga agatcaacag gatgagctca tcagaagcat cgagttggcc ctcacctcac 5041 aagacatcgc tgaagtcaca cagaccctct taaacttggc tgaattcatg gaacacagtg 5101 acaagggccc cctgccactg agagatgaca atggcattgt tctgctgggt gagagagctg 5161 ccaagtgccg agcatatgcc aaagcactac actacaaaga actggagttc cagaaaggcc 5221 ccacccctgc cattctagaa tctctcatca gcattaataa taagctacag cagccggagg 5281 cagcggccgg agtgttagaa tatgccatga aacactttgg agagctggag atccaggcta 5341 cctggtatga gaaactgcac gagtgggagg atgcccttgt ggcctatgac aagaaaatgg 5401 acaccaacaa ggacgaccca gagctgatgc tgggccgcat gcgctgcctc gaggccttgg 5461 gggaatgggg tcaactccac cagcagtgct gtgaaaagtg gaccctggtt aatgatgaga 5521 cccaagccaa gatggcccgg atggctgctg cagctgcatg gggtttaggt cagtgggaca 5581 gcatggaaga atacacctgt atgatccctc gggacaccca tgatggggca ttttatagag 5641 ctgtgctggc actgcatcag gacctcttct ccttggcaca acagtgcatt gacaaggcca 5701 gggacctgct ggatgctgaa ttaactgcaa tggcaggaga gagttacagt cgggcatatg 5761 gggccatggt ttcttgccac atgctgtccg agctggagga ggttatccag tacaaacttg 5821 tccccgagcg acgagagatc atccgccaga tctggtggga gagactgcag ggctgccagc 5881 gtatcgtaga ggactggcag aaaatcctta tggtgcggtc ccttgtggtc agccctcatg 5941 aagacatgag aacctggctc aagtatgcaa gcctgtgcgg caagagtggc aggctggctc 6001 ttgctcataa aactttagtg ttgctcctgg gagttgatcc gtctcggcaa cttgaccatc 6061 ctctgccaac agttcaccct caggtgacct atgcctacat gaaaaacatg tggaagagtg 6121 cccgcaagat cgatgccttc cagcacatgc agcattttgt ccagaccatg cagcaacagg 6181 cccagcatgc catcgctact gaggaccagc agcataagca ggaactgcac aagctcatgg 6241 cccgatgctt cctgaaactt ggagagtggc agctgaatct acagggcatc aatgagagca 6301 caatccccaa agtgctgcag tactacagcg ccgccacaga gcacgaccgc agctggtaca 6361 aggcctggca tgcgtgggca gtgatgaact tcgaagctgt gctacactac aaacatcaga 6421 accaagcccg cgatgagaag aagaaactgc gtcatgccag cggggccaac atcaccaacg 6481 ccaccactgc cgccaccacg gccgccactg ccaccaccac tgccagcacc gagggcagca 6541 acagtgagag cgaggccgag agcaccgaga acagccccac cccatcgccg ctgcagaaga 6601 aggtcactga ggatctgtcc aaaaccctcc tgatgtacac ggtgcctgcc gtccagggct 6661 tcttccgttc catctccttg tcacgaggca acaacctcca ggatacactc agagttctca 6721 ccttatggtt tgattatggt cactggccag atgtcaatga ggccttagtg gagggggtga 6781 aagccatcca gattgatacc tggctacagg ttatacctca gctcattgca agaattgata 6841 cgcccagacc cttggtggga cgtctcattc accagcttct cacagacatt ggtcggtacc 6901 acccccaggc cctcatctac ccactgacag tggcttctaa gtctaccacg acagcccggc 6961 acaatgcagc caacaagatt ctgaagaaca tgtgtgagca cagcaacacc ctggtccagc 7021 aggccatgat ggtgagcgag gagctgatcc gagtggccat cctctggcat gagatgtggc 7081 atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg aaaggcatgt 7141 ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact ctgaaggaaa 7201 catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt 7261 acatgaaatc agggaatgtc aaggacctca cccaagcctg ggacctctat tatcatgtgt 7321 tccgacgaat ctcaaagcag ctgcctcagc tcacatcctt agagctgcaa tatgtttccc 7381 caaaacttct gatgtgccgg gaccttgaat tggctgtgcc aggaacatat gaccccaacc 7441 agccaatcat tcgcattcag tccatagcac cgtctttgca agtcatcaca tccaagcaga 7501 ggccccggaa attgacactt atgggcagca acggacatga gtttgttttc cttctaaaag 7561 gccatgaaga tctgcgccag gatgagcgtg tgatgcagct cttcggcctg gttaacaccc 7621 ttctggccaa tgacccaaca tctcttcgga aaaacctcag catccagaga tacgctgtca 7681 tccctttatc gaccaactcg ggcctcattg gctgggttcc ccactgtgac acactgcacg 7741 ccctcatccg ggactacagg gagaagaaga agatccttct caacatcgag catcgcatca 7801 tgttgcggat ggctccggac tatgaccact tgactctgat gcagaaggtg gaggtgtttg 7861 agcatgccgt caataataca gctggggacg acctggccaa gctgctgtgg ctgaaaagcc 7921 ccagctccga ggtgtggttt gaccgaagaa ccaattatac ccgttcttta gcggtcatgt 7981 caatggttgg gtatatttta ggcctgggag atagacaccc atccaacctg atgctggacc 8041 gtctgagtgg gaagatcctg cacattgact ttggggactg ctttgaggtt gctatgaccc 8101 gagagaagtt tccagagaag attccattta gactaacaag aatgttgacc aatgctatgg 8161 aggttacagg cctggatggc aactacagaa tcacatgcca cacagtgatg gaggtgctgc 8221 gagagcacaa ggacagtgtc atggccgtgc tggaagcctt tgtctatgac cccttgctga 8281 actggaggct gatggacaca aataccaaag gcaacaagcg atcccgaacg aggacggatt 8341 cctactctgc tggccagtca gtcgaaattt tggacggtgt ggaacttgga gagccagccc 8401 ataagaaaac ggggaccaca gtgccagaat ctattcattc tttcattgga gacggtttgg 8461 tgaaaccaga ggccctaaat aagaaagcta tccagattat taacagggtt cgagataagc 8521 tcactggtcg ggacttctct catgatgaca ctttggatgt tccaacgcaa gttgagctgc 8581 tcatcaaaca agcgacatcc catgaaaacc tctgccagtg ctatattggc tggtgccctt 8641 tctggtaact ggaggcccag atgtgcccat cacgtttttt ctgaggcttt tgtactttag 8701 taaatgcttc cactaaactg aaaccatggt gagaaagttt gactttgtta aatgggggat 8761 ccactagttc tagagcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca 8821 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 8881 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 8941 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 9001 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 9061 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 9121 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 9181 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 9241 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 9301 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 9361 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 9421 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 9481 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 9541 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 9601 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 9661 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 9721 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 9781 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct 9841 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc 9901 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc 9961 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 10021 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 10081 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 10141 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 10201 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 10261 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 10321 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 10381 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 10441 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac 10501 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 10561 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 10621 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 10681 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 10741 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc 10801 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 10861 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 10921 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 10981 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 11041 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 11101 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 11161 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 11221 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 11281 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 11341 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 11401 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 11461 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 11521 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 11581 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 11641 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 11701 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 11761 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 11821 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 11881 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 11941 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 12001 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 12061 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 12121 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 12181 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 12241 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 12301 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 12361 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 12421 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 12481 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 12541 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 12601 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 12661 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 12721 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 12781 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 12841 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 12901 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 12961 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 13021 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 13081 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 13141 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 13201 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacg //