LOCUS Exported 2143 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.. ACCESSION . VERSION . KEYWORDS P1m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2143) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2143) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2143 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(84..944) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 707..726 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(945..1049) /gene="bla" /label=AmpR promoter primer_bind 1117..1135 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1139..1182 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 1185..1206 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 1271..1291 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(1297..1314) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1468..2056) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1548..1567) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 61 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 121 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 181 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg 241 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 301 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 361 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 421 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 481 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 541 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 601 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 661 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 721 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 781 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 841 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 901 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 961 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 1021 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 1081 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat 1141 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt 1201 ctagagacta gtgggtctca ggagtttaca gctagctcag tcctaggtat tatgctagct 1261 actagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc 1321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 1381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 1441 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca 1501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 1561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 1621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 1681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 1741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 1801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 1861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 1921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 1981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 2041 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 2101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaa //