LOCUS Exported 2239 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB for pSalTTTC (Adam Meyer's improved pSal promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).. ACCESSION . VERSION . KEYWORDS P60m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2239) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2239) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2239 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 161..180 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 414..431 /label=L4440 /note="L4440 vector, forward primer" misc_feature 437..457 /label=BioBrick suffix /note="universal suffix for all parts" misc_feature 618..639 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 642..685 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(689..707) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 775..879 /gene="bla" /label=AmpR promoter CDS 880..1740 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1098..1117) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1911..260 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 61 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 121 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 181 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 241 gggggcggag cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 301 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 361 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 421 cagtgagcga ggaagcctgc agcggccgct actagtaggt ctctagtagt taataacggt 481 aacggagcaa acaatataaa taaatacacc actcgatata taataaatca tcaacatgaa 541 tattgcgccc ggccgggcac cagcaataac ccaagcgagg cccccagcca acgatcgttc 601 tcctgagacc cactagtctc tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa 661 gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt ataggttaat 721 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 841 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 901 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 1021 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 1081 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 1141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 1201 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 1261 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 1321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 1381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 1441 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 1501 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 1561 tttattgctg ataaatctgg agccggtgag cgtgggtcgc gcggtatcat tgcagcactg 1621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 1681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 1741 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 1801 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 1861 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 1921 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 1981 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 2041 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 2101 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 2161 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 2221 tcgggctgaa cggggggtt //