LOCUS Exported 2783 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file. ACCESSION . VERSION . KEYWORDS C9m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2783) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2783) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2783 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 50..67 /label=L4440 /note="L4440 vector, forward primer" misc_feature 73..93 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(123..144) /label=EGFP-C /note="EGFP, forward primer" primer_bind 472..491 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 731..752 /label=EGFP-N /note="EGFP, reverse primer" misc_feature 798..819 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 822..865 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(869..887) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 955..1059 /gene="bla" /label=AmpR promoter CDS 1060..1920 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1278..1297) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2091..2679 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2580..2599 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 61 gagcgaggaa gcctgcagcg gccgctacta gtaggtctct acctttagat cccggcggcg 121 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg 181 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg 241 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg 301 aagttggcct tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgatg 361 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc 421 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag 481 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac 541 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccccag 601 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac 661 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg 721 tggccgttta cgtcgccgtc cagctcgacc aggatgggaa caacgccagt gaaaagctcc 781 atttgagacc cactagtctc tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa 841 gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt ataggttaat 901 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 1021 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 1081 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 1141 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 1201 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 1261 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 1321 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 1381 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 1441 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 1501 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 1561 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 1621 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 1681 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 1741 tttattgctg ataaatctgg agccggtgag cgtgggtcgc gcggtatcat tgcagcactg 1801 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 1861 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 1921 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 1981 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 2041 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 2101 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 2161 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 2221 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 2281 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 2341 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 2401 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 2461 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 2521 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 2581 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 2641 tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa cgcggccttt 2701 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 2761 gattctgtgg ataaccgtat tac //