LOCUS Exported 2861 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/). ACCESSION . VERSION . KEYWORDS C20m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2861) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2861) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2861 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 740..760 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(766..783) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(937..1525) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1017..1036) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1696..2556) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2319..2338 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2557..2661) /gene="bla" /label=AmpR promoter primer_bind 2729..2747 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2751..2794 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2797..2818 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS 2834..686 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" ORIGIN 1 gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc 61 gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta 121 ctggtaaact gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt 181 gctttgctcg ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg 241 aaggctatgt gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg 301 cggaagtgaa atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact 361 ttaaagaaga cggcaatatc ctgggccata agctggaata caattttaac agccacaatg 421 tttacatcac cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca 481 acgtggagga tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg 541 atggtcctgt tctgctgcca gacaatcact atctgagcta ccaaagcgtt ctgtctaaag 601 atccgaacga gaaacgcgat catatggttc tgctggagtt cgtaaccgca gcgggcatca 661 cgcatggtat ggatgaactg tacaaaacta gtgcagcgaa cgacgaaaat tacgcccttg 721 cagcgtgaag gtagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc 781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga 961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 1021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 1081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 1141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 1201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 1261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 1321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 1381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 1441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1501 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 1561 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1621 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1681 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 1741 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 1801 cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga 1861 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 1921 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 1981 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 2041 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 2101 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 2161 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 2221 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 2281 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 2341 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 2401 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 2461 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 2521 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 2581 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 2641 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 2701 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata 2761 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac 2821 tagtgggtct caaatgcgta aaggcgaaga gctgttcact g //