LOCUS Exported 10739 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.. ACCESSION . VERSION . KEYWORDS pJW1595 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10739) AUTHORS Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD TITLE An expanded auxin-inducible degron toolkit for Caenorhabditis elegans JOURNAL Genetics, 2021 REFERENCE 2 (bases 1 to 10739) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10739 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(29..51) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 151..170 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 364..386 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 378..395 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 661..966 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" CDS 1887..1907 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" protein_bind 1925..1958 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 1968..1997 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" terminator 3814..4137 /gene="tbb-2" /label=tbb-2 terminator /note="transcription terminator for the C. elegans tubulin beta-2 gene" protein_bind 6055..6079 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6082..7107 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" protein_bind complement(7108..7132) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind 7485..7518 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS 7819..7842 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 7846..7869 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 7873..7896 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS complement(7944..8249) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(8518..8534) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8518..8534) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8531..8553) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8542..8558 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8566..8596) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8611..8632 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8749..8766) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8920..9508) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9000..9019) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9679..10539) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10302..10321 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10540..10644) /gene="bla" /label=AmpR promoter primer_bind 10712..10730 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat 421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat 481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga 541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa 661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg 1021 gagcctcagg agcatcgatg gtctccgagc tcattaaaga aaacatgcat atgaagctct 1081 acatggaggg aaccgtcaac aaccaccact tcaagtgcac ctccgaggga gagggaaagc 1141 catacgaggg aacccaaacc atgcgtatca aggccgtcga gggaggacca ctcccattcg 1201 ccttcgacat cctcgccacc tccttcatgt acggatccaa ggtaagttta aacatatata 1261 tactaactaa ccctgattat ttaaattttc agaccttcat caaccacacc caaggaatcc 1321 cagacttctt caagcaatcc ttcccagagg gattcacctg ggagcgtgtc accacctacg 1381 aggacggagg agtcctcacc gccacccaag acacctccct ccaagacgga tgcctcatct 1441 acaacgtcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc 1501 agatccgtgg agtcaacttc ccatccaacg gaccagtcat gcaaaagaag accctcggat 1561 gggaggcctc caccgagacc ctctacccag ccgacggagg acttgaggga cgtgccgaca 1621 tggccctcaa gctcgtcgga ggaggacacc tcatctgcaa cctcaaggta agtttaaaca 1681 tgattttact aactaactaa tctgatttaa attttcagac cacctaccgt tccaagaagc 1741 cagccaagaa cctcaagatg ccaggagtct actacgtcga ccgtcgtctt gagcgtatca 1801 aggaggccga caaggagacc tacgtcgagc aacacgaggt cgccgtcgcc cgttactgcg 1861 acctcccatc caagctcgga caccgtgaga atctgtactt tcaatccgga aaggtaagtt 1921 taaaataact tcgtatagca tacattatac gaagttattt tcagggagag caaaagctta 1981 tctccgagga ggacctctaa ggatgatcga cgccaacgtc gttgaatttt caaattttaa 2041 atactgaata tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg 2101 ctttctaaaa aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca 2161 attcgtattc cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt 2221 catttcatta ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga 2281 aatgaaataa ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt 2341 aaaagaaaac ataaaaatat atagaggagg aaggaaacta gctgccctcg attgtctatc 2401 ttccattctc accaagtttc aacaacttta ttcagtcatt cttctctggt tcatttccca 2461 accaatcttc ccgaataaag cccatctgtc ctttcggcca cattcctcac ctacatccgt 2521 actccttatc tcccgttctt atccagtctt ttatcgtctc tgcgtctcgc acactgtctc 2581 gtcggtaatg taaaaaaaaa acaaaaaaaa cttgaaaact tgcggaaaga tttcctaata 2641 aaaggagacg aatccggtag gaaatcgaca agtctctgct tctcgaagac taggtgagtt 2701 acatttcttt ggccgaatta ttttgaaatc ctaaatttga taatttcaga gatgtctgta 2761 aaacttgcgt gttatgtgac ggcttcggtc actgtcgcca ctcttatggt atgcttcatg 2821 accatgtcaa ccatctactc ggaggttgat ggattcagag agaagcttga taccgagatg 2881 aatgtgttca gagtgagtta aattatattt tgaattttaa ataattttta attttctagc 2941 aatctaccaa tggattgtgg aaggacatag ttgtcatcgg aagatctagc aagcgtgtcc 3001 gttgtcaata tgaagagacc aacgctaccc caactccaca tgctgatgga tccccatctg 3061 ctccaccagg tcaaccacca gcagttccac cagtcttcaa ccagccaaag actccaaatg 3121 gagccaatgg aaatggacca acctgcaact gcaatgctga taacaagtgc ccagctggac 3181 catccggacc aaagggagtt ccaggagttc caggactcga cggagttcca ggacttgacg 3241 gtgttccagg agttggagct gatgatatcg ctccacaacg cgagtctgtc ggatgcttca 3301 cttgcccaca aggaccagtt ggaccaccag gagctcttgg aagaccagga ccacgtggac 3361 ttccaggacc aagaggacaa aatggaaacc caggaagaga tggacaacca ggacatccag 3421 gagagcaagg atcatccggt caaatcggaa agatcggaga gccaggacca ccaggagaga 3481 agggacgcga cgccgagcat ccaatcggaa gaccaggacc aaagggacca agaggagatc 3541 aaggaccaac aggaccagct ggacagaacg gtcttcacgg accaccagga gagccaggaa 3601 ccgttggacc agaaggacca tctggaaagc aaggacgtca aggaccagac ggaacccagg 3661 gagagactgg accagacgga agaccaggaa aggatgccga gtactgccag tgcccagaca 3721 agtctccacc atcagaggct gtcaacgcca accgtggata cagaaatatc taaattgttg 3781 gtgttttcta ataaaatatt tgagattcag tgagactttt ttcttggcgg cacaataaag 3841 tttcctcttt ttgttgggtc actcaacgaa cacaatacaa atatttgagc gctcgcattt 3901 ttctcaacct ttcgggttta aagcacttca aaatggaatt gaagagagat tgtgaaagta 3961 catcagacag aatcacattt tgatagcatt cacttcactc agatgcaaac ttttcagtga 4021 ctataaaaag tcataaaata ataacattaa aaaagcaaat aaattagcga gagaattttt 4081 tgacaaaaag aaagaagagt gatagagaag aagggaatgc ttgaaaggat tttgcattta 4141 tcttaatcac cgtcttcgag aagacgaacc atggcgccag tttcagaatc caagtttcta 4201 atatagttca tcacaatgtt gacgttggtc caacctccgg cttgcataat ctctgggatg 4261 gaaactccag cgcgagccat gtcacgggca gctccgacac gggcggaatg tccagaccaa 4321 gccaagtaac gttgtccgga atcatccttg gctccgtaga tgagacggtg ggtagcttcg 4381 aagattcctg aaaatttatt ttcagcttat tcttagctaa tatgttgttt aaacttacct 4441 tcaagagctc gagtcgagag ttgtgatgtt gcactcgggg cagcaactcc gttcttgcga 4501 acacgacaga acaggtagtt gtttggatca tcggcgactc cggaaaccga aatccagcgc 4561 tcaacgagct tggtaactcc aagggagagc gctttttcga ctccggcagt cgagaccagc 4621 gtttttgtac gtccgatgtg gatgagcatt cttcctccgt ctgtgcgact aatatccttg 4681 acgcgaatgc gggcaatttc ggcgatacgg agaagtgtgt tgtaagcaat tccgaggaag 4741 gcgaggtttc ggatgtcctg gcaacgatcg gaattttcca taagagaacg gacctgatcg 4801 aagtcggttc tgaaaatttc gtcaagtctt aggattgaat acattaatat ttaaacttac 4861 ctttcgaagg cgagggcttg cttggctctc tctccagcat caacattttc cttgcggata 4921 cgtctcatta cgagagatac ggcattagaa tctgatgggc gtggaagtcc agatcggcga 4981 tggagcatat tgagctgtcc gagatgttgt tggatcgtct tcaccgccaa tccacgagct 5041 tggaggtaga gcaagtagtc acgaacgtct tctggctcgg ctgggaacca cttgcggttg 5101 ttaagcttgc accaggcagc ccaagaacgg cacactgaga gaagcatctt ccaggtgtgc 5161 tcggagaagg cttgtctgtc acggaacata tccatgaggt tcttgcgaac ttcatcactt 5221 gtcgcgtcaa ctggaagtgc tggaagattt tgatgaacag tgagaagatt tgacatattt 5281 tcgaagtttt ttagatgcac tagaacaaag cgtgttggct tcctctgagc ccgctttcct 5341 tatatacccg cattctgcag ccttacagaa tgttctagaa ggtcctagat gcattcgttt 5401 gaaaatactc ccggtgggtg caaagagacg cagacggaaa atgtatctgg gtctctttat 5461 tgtgtacact acttttccat gtaccgaatg tgagtcgccc tccttttgca acaagcagct 5521 cgaatgttct agaaaaaggt ggaaaatagt ataaataccg ttgaaaataa ataccgaaca 5581 acatttgctc taattgtgaa attagaaatc ttcaaactat aatcatgtca ctttaccact 5641 atttccgtcc agctcaacgt tccgtttttg gtgatcattt ttgctttcgt cgtaaatcta 5701 cacacgcgtc tcttccgtgc gagagtccaa gccagcagcc aaattcgttg actgagtatt 5761 caacgtttat acgttgtcgg caacgagaaa taggaaaatg catcgggaaa tgttcttttt 5821 tcgatttttt ccaaggtttt gacaaatttt accacgaatt ttgctatgtt ttcaattaaa 5881 aaatatgtta ttcaactgtt tctatgagga aaataaggct ttgcatgtaa ttttcttatt 5941 cagcataatt tttaattaat ttgaattttc tgtcctaacg tttattttgt tttcttggtt 6001 atgactgatc tgaaattaat ttttgaattt taaggtaata tgtcaggcgg tgccgcaagt 6061 ttgtacaaaa aagcaggctc catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag 6121 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa 6181 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc 6241 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg 6301 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc 6361 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag 6421 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc 6481 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg 6541 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc 6601 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac 6661 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg 6721 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc 6781 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat 6841 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa 6901 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc 6961 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc 7021 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc 7081 agcactcgtc cgagggcaaa ggaatagacc cagctttctt gtacaaagtg ggtccaatta 7141 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta 7201 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg 7261 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct 7321 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc 7381 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa 7441 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttaataact tcgtatagca 7501 tacattatac gaagttattt tcagggagcc ggatctgatt acggatccgg aggtggcggg 7561 atggcttctt ctcttcgtca gatcctcgac tctcagaaga tggagtggcg ttctaacgct 7621 ggaggatctg gcggaggagg cagtggtagt ggcagtatgc ctaaagatcc agccaaacct 7681 ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa gaacgtgatg 7741 gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa gggtggtggc 7801 ggttctggct caggatctga ttataaagac gatgacgata agcgtgacta caaggacgac 7861 gacgacaagc gtgattacaa ggatgacgat gacaagagac tagttcgacc tgcagactgg 7921 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg 7981 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca 8041 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa 8101 agttcacggg ggactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt 8161 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct 8221 cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cggcaaaaga 8281 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca 8341 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttaccg aaccggagat 8401 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata 8461 cgctgcttca tagcatacct ctttttgaca tacttcgggt tcctaggaaa tcgataagtc 8521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 8581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 8641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 8701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 8761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 8821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 8881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 8941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 9001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 9061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 9121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 9181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 9241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 9301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 9361 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 9421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 9481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 9541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 9601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 9661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 9721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 9781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 9841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 9901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 9961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 10021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 10081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 10141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 10201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 10261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 10321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 10381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 10441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 10501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 10561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 10621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 10681 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc //