LOCUS Exported 3583 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. ACCESSION . VERSION . KEYWORDS SuperNova/pRSETB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3583) AUTHORS Takemoto K, Matsuda T, Sakai N, Fu D, Noda M, Uchiyama S, Kotera I, Arai Y, Horiuchi M, Fukui K, Ayabe T, Inagaki F, Suzuki H, Nagai T TITLE SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. JOURNAL Sci Rep. 2013;3:2629. doi: 10.1038/srep02629. PUBMED 24043132 REFERENCE 2 (bases 1 to 3583) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3583 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..42 /label=T7 /note="T7 promoter, forward primer" promoter 23..41 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 73..95 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 115..132 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 136..168 /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /label=T7 tag (gene 10 leader) /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS 172..195 /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /label=Xpress(TM) tag /translation="DLYDDDDK" CDS 205..924 /codon_start=1 /product="monomeric variant of the photosensitizing red fluorescent protein KillerRed (Takemoto et al., 2013)" /label=SuperNova /translation="MGSEVGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPSETHMFPHGPNAVRQTA TIGFTTADGGKMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQTRDTSDKRDHVCQREVA YAHSVPRITSAIGSDED" primer_bind complement(987..1005) /label=T7 Term /note="T7 terminator, reverse primer" terminator 1001..1048 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 1145..1600 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1232..1251) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1442..1463 /label=F1ori-F /note="F1 origin, forward primer" promoter 1626..1730 /gene="bla" /label=AmpR promoter CDS 1731..2591 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1949..1968) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2762..3350 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3251..3270 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3504..3521 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 caggatctcg atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc 61 tctagaaata attttgttta actttaagaa ggagatatac atatgcgggg ttctcatcat 121 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac 181 gacgatgacg ataaggatcc caccatgggt tcagaggtcg gccccgccct gttccagagc 241 gacatgacct tcaaaatctt catcgacggc gaggtgaacg gccagaagtt caccatcgtg 301 gccgacggca gcagcaagtt cccccacggc gacttcaacg tgcacgccgt gtgcgagacc 361 ggcaagctgc ccatgagctg gaagcccatc tgccacctga tccagtacgg cgagcccttc 421 ttcgcccgct accccgacgg catcagccat ttcgcccagg agtgcttccc cgagggcctg 481 agcatcgacc gcaccgtgcg cttcgagaac gacggcacca tgaccagcca ccacacctac 541 gagctggacg acacctgcgt ggtgagccgc atcaccgtga actgcgacgg cttccagccc 601 gacggcccca tcatgcgcga ccagctggtg gacatcctgc ccagcgagac ccacatgttc 661 ccccacggcc ccaacgccgt gcgccagacc gccaccatcg gcttcaccac cgccgacggc 721 ggcaagatga tgggccactt cgacagcaag atgaccttca acggcagccg cgccatcgag 781 atccccggcc cacacttcgt gaccatcatc accaagcaga cgagggacac tagcgacaag 841 cgcgaccacg tgtgccagcg cgaggtggcc tacgcccaca gcgtgccccg catcaccagc 901 gccatcggta gcgacgagga ttaagaattc gaagcttgat ccggctgcta acaaagcccg 961 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc 1021 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatctggcgt 1081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 1141 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 1201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 1261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 1321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 1381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 1441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 1501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 1561 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 1621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 1681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 1741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 1801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 1861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 1921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 1981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 2041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 2101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 2161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 2221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 2281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 2341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 2401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 2461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 2521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 2581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 2641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 2701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 2941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 3181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 3361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 3421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 3481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 3541 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atg //