LOCUS Exported 3374 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway entry vector containing mouse Ovol1.. ACCESSION . VERSION . KEYWORDS pENTR-mOvol1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3374) AUTHORS Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, Schroeder T, Woltjen K TITLE OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates. JOURNAL Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 10.1016/j.stemcr.2018.12.008. PUBMED 30639212 REFERENCE 2 (bases 1 to 3374) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3374 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1499..1598) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1615..1634) /label=T7 /note="T7 promoter, forward primer" promoter complement(1616..1634) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1639..1655) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1639..1655) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1757..1776) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1768..2577 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1835..1854) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2724..3312 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3213..3232 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gcggccgcca ccatgcctcg cgcgttcctg gtgaagaagc catgcgtctc 721 cacgtgcaag aggaactgga gcgagctccc ggacgaagag cgcggcgaga tctacgtgcc 781 agtcagcctc ggcttctgcc caccacagcc ctaccgggaa ccagaggcat cggtggccga 841 gcctccttcc tgccccctgg ctttggacat gagccttcga gactccagct atagcgtggc 901 tccagggccc tgcgtggtgg cccagctacc ctctgaagat gtgagccatc tgacagatcc 961 ccagagcaga gaccagggct ttctacgaac caagatgaag gtgaccttgg gggacagtcc 1021 aaatggagac ctttttacct gccacatctg ccagaagtcc ttcacccacc agcgcatgct 1081 gaatcgacac atgaagtgtc acaacgacgt caagaggcac ctctgcactt actgtgggaa 1141 gggcttcaat gacacctttg acctcaagag acatgtccga acccacactg gtgtgcggcc 1201 ctacaagtgc agtctgtgtg ataaggcctt cactcagcgc tgctctctgg agtcccacct 1261 caagaagatc cacggagtgc agcagaagta tgcatacaag gagcgccggg ccaagctcta 1321 cgtgtgtgag gagtgcggtt gcacatccga gagtcaagag ggtcatgtcc tgcacctcaa 1381 ggaacgccac cccgacagcc cactgctgcg caagacctcc aagaaagtgg ctgtggctct 1441 gcagaacact gtcacgtccc tgctgcaggg cagcccccat ctctgaagag gcgcgccgac 1501 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac 1561 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat 1621 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat 1681 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct 1741 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 1801 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 1861 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 1921 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 1981 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 2041 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 2101 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 2161 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 2221 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 2281 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 2341 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 2401 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 2461 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 2521 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 2581 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca 2641 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc 2701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 2761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 2821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 2881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 2941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 3001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 3061 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 3121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 3181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 3241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 3301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 3361 tttgctcaca tgtt //