LOCUS Exported 3101 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C38m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3101) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 3101) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3101 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 5..23 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 27..70 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 73..94 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 1117..1137 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(1143..1160) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1314..1902) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1394..1413) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2073..2933) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2696..2715 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2934..3038) /gene="bla" /label=AmpR promoter ORIGIN 1 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa 61 ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctcaaa tgatgagtca 121 gaatacgctg aaagttcatg atttaaatga agatgcggaa tttgatgaga acggagttga 181 ggtttttgac gaaaaggcct tagtagaaca ggaacccagt gataacgatt tggccgaaga 241 ggaactgtta tcgcagggag ccacacagcg tgtgttggac gcgactcagc tttaccttgg 301 tgagattggt tattcaccac tgttaacggc cgaagaagaa gtttattttg cgcgtcgcgc 361 actgcgtgga gatgtcgcct ctcgccgccg gatgatcgag agtaacttgc gtctggtggt 421 aaaaattgcc cgccgttatg gcaatcgtgg tctggcgttg ctggacctta tcgaagaggg 481 caacctgggg ctgatccgcg cggtagagaa gtttgacccg gaacgtggtt tccgcttctc 541 aacatacgca acctggtgga ttcgccagac gattgaacgg gcgattatga accaaacccg 601 tactattcgt ttgccgattc acatcgtaaa ggagctgaac gtttacctgc gaaccgcacg 661 tgagttgtcc cataagctgg accatgaacc aagtgcggaa gagatcgcag agcaactgga 721 taagccagtt gatgacgtca gccgtatgct tcgtcttaac gagcgcatta cctcggtaga 781 caccccgctg ggtggtgatt ccgaaaaagc gttgctggac atcctggccg atgaaaaaga 841 gaacggtccg gaagatacca cgcaagatga cgatatgaag cagagcatcg tcaaatggct 901 gttcgagctg aacgccaaac agcgtgaagt gctggcacgt cgattcggtt tgctggggta 961 cgaagcggca acactggaag atgtaggtcg tgaaattggc ctcacccgtg aacgtgttcg 1021 ccagattcag gttgaaggcc tgcgccgttt gcgcgaaatc ctgcaaacgc aggggctgaa 1081 tatcgaagcg ctgttccgcg agtaaaggta gagacctact agtagcggcc gctgcaggct 1141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac 1321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 2041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 2101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 2161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgcgaccca 2221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 2341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 2401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 2701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3061 cctgacgtct aagaaaccat tattatcatg acattaacct a //