LOCUS       Exported                4928 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of the smooth muscle version of myocardin.
ACCESSION   .
VERSION     .
KEYWORDS    Sm-Myocardin Omni-pShuttle
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Zhou J, Zhang M, Fang H, El-Mounayri O, Rodenberg JM, Imbalzano AN, 
            Herring BP
  TITLE     The SWI/SNF chromatin remodeling complex regulates myocardin-induced
            smooth muscle-specific gene expression.
  JOURNAL   Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 
            10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2.
  PUBMED    19342595
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             33..62
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             66..89
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(2734..2751)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2740..2964
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3129..3146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3300..3888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3380..3399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4110..4129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4301..4680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4681..4884
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4835..4855
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 taatacgact cactataggg agacccaagc tggctagcat gactggtgga cagcaaatgg
       61 gtcgggatct gtacgacgat gacgataagg ggccctctag acacattctc caagcctcca
      121 cggcagaaag gtccattcca actgctcaga tgaagctcaa aagagcccgc cttgcagatg
      181 acctcaatga gaagatcgct ctccgcccag ggcccttgga actggtggag aagaacattc
      241 tgccgatgga ttcttccgtg aaagaggcta taaaaggtac tgaggtgagc ctctccaagg
      301 cagcagatgc attcgccttt gaggatgaca gcagtagaga tgggctctct ccagatcagg
      361 ctaggagcga ggacccccag ggctctacag gatccacccc agacatcaaa tccactgagg
      421 ctcctctgga cacaatccag gatctcactc ctggctcaga aagtgacaag aatgatgcag
      481 cctcccagcc aggcaaccag tcagaccctg ggaagcaggt tctcggcccc ctcagcaccc
      541 cgattcctgt gcacactgct gtaaagtcca agtctttggg tgacagtaag aaccgccaca
      601 aaaagcccaa agaccccaaa ccaaaggtga agaagctcaa ataccatcag tacatccccc
      661 cagaccagaa ggcagagaag tctcccccac ccatggactc tgcctatgcc cggctgctcc
      721 agcaacagca gctattcctg cagctacaga tcctcagcca gcagcagcaa cagcagcagc
      781 aacagcagca gcagcaacag cagcagcagc agcagcagca gcggttcagc taccctggga
      841 tgcaccaaac acacctcaaa gaaccaaatg aacagatggc cagaaatccg aatccttctt
      901 caacaccact gagcaatacc cctctatccc ctgtcaaaaa tagcatttct ggacaaactg
      961 gtgtttcttc tctcaaacca ggccccctcc cacccaacct ggatgatctc aaggtgtcag
     1021 agttaagaca acagcttcga atccggggct tgccagtgtc aggcaccaag acagcgctgg
     1081 tggaccggct tcgtcccttc caggattgtg ctggcaaccc tgtgcccaac tttggggaca
     1141 tcacaactgt cacctttcct gtcacgccca acaccttgcc cagttatcag tcctccccga
     1201 caggcttcta ccactttggc agcacaagct ccagcccacc catctccccc gcctcatctg
     1261 acttgtccgc tgcagggtcc ctgccagaca ccttcaccga tgcgtcacct ggcttcggcc
     1321 tgcacgcatc tccggtgccc acctgcacgg acgagagtct gctgagcagc ctgaatgggg
     1381 gctcgggccc ctccgagcct gatgggctag actctgagaa ggacaagatg ctggtggaga
     1441 agcagaaagt gatcaaccag ctcacctgga agctgcggca agagcagcgg caggtggaag
     1501 agctgagaat gcaactgcag aagcagaaga gcagctgcag cgaccagaag ccactgccct
     1561 tcttggccac caccatcaaa caggaagatg tctccagctg ccccttcgca ccccagcagg
     1621 cgtctgggaa gggacagggc cacagctctg acagtccccc tccggcttgt gagacggctc
     1681 agctgctgcc tcactgtgtg gagtcctcag gtcaaaccca tgtactctcg tccacgtttc
     1741 tcagccccca gtgctcccct cagcactcgc ccctgggggg cctgaagagc ccgcagcaca
     1801 tcagcctgcc tccatcaccc aacaaccatt acttcctggc ttcctcttcg ggagctcaga
     1861 gagagaacca tggggtctct tcacccagca gcagccaagg gtgcgcacag atgactggtt
     1921 tacaatcttc tgacaaggtg gggccaacgt tttcaattcc atccccaact ttttctaagt
     1981 caagttcagc agtttcagat atcacccagc ccccatccta tgaagatgca gtgaagcagc
     2041 aaatgactcg gagtcagcag atggacgaac tcctggatgt cctcattgaa agtggagaaa
     2101 tgccagccga tgccagggaa gatcattcat gtcttcagaa aattccaaag atccctgggt
     2161 cctcctgcag cccaactgcc atccccccga agccctcggc ttcctttgag caggcatctt
     2221 cgggaggcca gatggccttc gatcactacg ccaacgacag tgacgaacac ctggaagtct
     2281 tattgaattc tcacagcccc atcggaaagg tgagcgatgt taccctcctc aaaatcggaa
     2341 gcgaggagcc tccttttgac agcatcatgg atggcttccc agggaaggct gcggaagatc
     2401 tcttcagtgc tcacgagctc ttgcctgggc ccctctcccc gatgcatgca cagttgtcac
     2461 ctccttctgt ggacagcagt ggtctgcagc tgagcttcac ggaatctcct tgggaaacaa
     2521 tggaatggct ggacctcact ccacctagtt ccacgccagg cttcagcaac cttacctcca
     2581 gtgggcccag cattttcaac atcgattttc tggatgttac agatcttaat ctgaattccc
     2641 ctatggatct ccacttacag cagtggtaag cggccgccac tgtgctggat gatccgagct
     2701 cggtaccaag cttaagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc
     2761 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2821 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2881 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2941 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagcaga tctgcagatc
     3001 tgaattcatc tatgtcgggt gcggagaaag aggtaatgaa atggcattat gggtattatg
     3061 ggtctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4021 tttccccgaa aagtgccacc tgacgtaact ataacggtcc taaggtagcg aaagctcaga
     4081 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4141 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     4201 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     4261 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     4321 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4381 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4441 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4501 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4561 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4621 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4681 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4741 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4801 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4861 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     4921 atcgaaat
//
