LOCUS       Exported                7387 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses rat neonatal Fc receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCB6HindIII-rat FcRn
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Tesar DB, Tiangco NE, Bjorkman PJ
  TITLE     Ligand valency affects transcytosis, recycling and intracellular 
            trafficking mediated by the neonatal Fc receptor.
  JOURNAL   Traffic. 2006 Sep;7(9):1127-42.
  PUBMED    17004319
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        137..516
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        517..720
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     671..691
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1540..1551
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     1908..1924
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1909..1925
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1965..2587
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2164..2183)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2611..2631)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2629..2926
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     2858..2877
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2980..3774
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3034..3053)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3644..3663
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4426..4560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4463..4482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4517..4536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4565..4584)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4566..4584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4598..4614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4598..4614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4611..4633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4622..4638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4646..4676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4691..4712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4829..4846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5000..5588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5080..5099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5759..6619)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6382..6401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6620..6724)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6751..7206
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6838..6857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7048..7069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7332..7354
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7346..7363
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7347..7363
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
       61 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
      121 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      181 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      241 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      301 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      361 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
      421 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
      481 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
      541 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      601 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
      661 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      721 cgtttagtga accgtcagaa ttaattcaga tctggtaccg tcgatccggt cacgtgtgcc
      781 ttccactccg ggtcgccctg tcaggatggg gatgtcccag cccggggtcc tcctcagcct
      841 cttattggtc ctcctgcctc agacctgggg agcggagccc cgtctcccac tgatgtatca
      901 tcttgcagct gtgtctgact tatcaacggg gcttccctct ttctgggcca cgggctggct
      961 gggtgctcag caatatctga cctacaacaa cctgcggcag gaggctgacc cctgtggggc
     1021 ctggatatgg gaaaaccagg tgtcttggta ttgggagaag gagaccacgg atctgaaaag
     1081 caaagaacag ctcttcttgg aggccatcag gaccctggag aaccaaataa atgggacctt
     1141 cacactgcag ggcctgctgg gctgtgaact ggcccctgat aattcttcat tgcccacggc
     1201 tgtgtttgcc ctcaatggtg aggagttcat gcggttcaac ccaagaacgg gcaactggag
     1261 tggggagtgg ccggagacag atatcgttgg taatctgtgg atgaagcaac ctgaggcggc
     1321 caggaaggag agcgagttcc tgctaacttc ttgtcctgag cggctgctag gccacctgga
     1381 gaggggccgt cagaacctgg agtggaagga gccgccatct atgcgcctga aggcccgtcc
     1441 tggcaactct ggctcctcag tactgacctg tgctgctttc tccttctacc cgccggagct
     1501 caagtttcga ttcctgcgca atgggctagc ctcaggctct gggaattgca gcactggtcc
     1561 caatggtgat ggatctttcc atgcatggtc attgctagag gtcaaacgtg gagatgaaca
     1621 ccattaccaa tgtcaagtgg agcatgaggg gctggcccag cctctcactg tggacctaga
     1681 ttcgcccgcc agatcttctg tgcctgtggt cggaatcatt cttggtttat tgctggtggt
     1741 agtggccatc gcagggggtg tgctgctatg gaacaggatg cgaagtgggc tgccagcccc
     1801 atggctttct ctcagtggtg atgactctgg cgacctattg cctggtggga acttgccccc
     1861 ggaggctgaa cctcaaggtg taaatgcctt tccggccact tcctgactcg aggtcgacgg
     1921 tatcgataag cttgcatgcc tgcaggtcga ctctagagga tcccgggtgg catccctgtg
     1981 acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg
     2041 tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg
     2101 ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg
     2161 gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
     2221 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
     2281 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg
     2341 ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc
     2401 tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat
     2461 accagcagga ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac
     2521 atttgagttg cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg
     2581 ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2641 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2701 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2761 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     2821 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     2881 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggagga attgatcagc
     2941 ttgggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3001 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3061 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     3121 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     3181 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3241 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3301 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     3361 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     3421 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     3481 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     3541 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     3601 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     3661 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     3721 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     3781 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     3841 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     3901 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccggg ctcgatcccc
     3961 tcgcgagttg gttcagctgc tgcctgaggc tggacgacct cgcggagttc taccggcagt
     4021 gcaaatccgt cggcatccag gaaaccagca gcggctatcc gcgcatccat gcccccgaac
     4081 tgcaggagtg gggaggcacg atggccgctt tggtcgaccc ggacgggacg ctcctgcgcc
     4141 tgatacagaa cgaattgctt gcaggcatct catgagtgtg tcttcccgtt ttccgcctga
     4201 ggtcactgcg tggatggagc gctggcgcct gctgcgcgac ggcgagctgc tcaccaccca
     4261 ctcgccaagc tggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccg
     4321 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     4381 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     4441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4561 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa
     4621 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4681 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4741 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6721 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     6781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6961 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     7021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     7081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7141 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7201 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     7261 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     7321 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgatctatac
     7381 attgaat
//
