LOCUS Exported 5928 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses rat beta-2-microglobulin in mammalian cells. ACCESSION . VERSION . KEYWORDS pBJ1-rat Beta2m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5928) AUTHORS Tesar DB, Tiangco NE, Bjorkman PJ TITLE Ligand valency affects transcytosis, recycling and intracellular trafficking mediated by the neonatal Fc receptor. JOURNAL Traffic. 2006 Sep;7(9):1127-42. PUBMED 17004319 REFERENCE 2 (bases 1 to 5928) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5928 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 108..487 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 488..691 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 642..662 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" polyA_signal 1174..1796 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(1373..1392) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(1820..1840) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1838..2135 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind 2067..2086 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2189..2983 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2243..2262) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2853..2872 /label=Neo-F /note="Neomycin resistance gene, forward primer" rep_origin complement(3512..4100) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3592..3611) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4271..5131) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4894..4913 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5132..5236) /gene="bla" /label=AmpR promoter rep_origin 5263..5718 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5350..5369) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5560..5581 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 5844..5866 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 5858..5875 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 5859..5875 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca 61 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt 121 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 181 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 241 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 301 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 361 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 421 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 481 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg 541 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 601 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg 661 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga attaattcag 721 atctggtacc gggccccccc tcgaggtcga tccacgatgg ctcgctcggt gaccgtgatc 781 tttctggtgc ttgtctctct ggccgtcgtg cttgccattc agaaaactcc ccaaattcaa 841 gtgtactctc gccatccacc ggagaatggg aagcccaact tcctcaactg ctacgtgtct 901 cagttccacc cacctcagat agaaattgag ctactgaaga atggaaagaa gataccaaat 961 atcgagatgt cagatctgtc cttcagcaag gactggtctt tctacatcct ggctcacact 1021 gaattcacac ccaccgagac cgatgtatat gcttgcagag ttaaacacgt cactctgaag 1081 gagcccaaaa ccgtcacctg ggaccgagac atgtaatcag gatcgacggt atcgataagc 1141 ttgcatgcct gcaggtcgac tctagaggat cccgggtggc atccctgtga cccctcccca 1201 gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt cctaataaaa 1261 ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg gtggaggggg 1321 gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg ggtctattgg 1381 gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt 1441 caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg 1501 ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc caggctggtc 1561 tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct gggattacag 1621 gcgtgaacca ctgctccctt ccctgtcctt ctgattttaa aataactata ccagcaggag 1681 gacgtccaga cacagcatag gctacctggc catgcccaac cggtgggaca tttgagttgc 1741 ttgcttggca ctgtcctctc atgcgttggg tccactcagt agatgcctgt tgaattgggt 1801 acgcggccag cttggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 1861 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 1921 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 1981 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 2041 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 2101 gagctattcc agaagtagtg aggaggcttt tttggaggaa ttgatcagct tgggatctga 2161 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 2221 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 2281 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 2341 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 2401 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 2461 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 2521 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 2581 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 2641 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 2701 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 2761 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 2821 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 2881 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 2941 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 3001 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 3061 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 3121 cgcggggatc tcatgctgga gttcttcgcc caccccgggc tcgatcccct cgcgagttgg 3181 ttcagctgct gcctgaggct ggacgacctc gcggagttct accggcagtg caaatccgtc 3241 ggcatccagg aaaccagcag cggctatccg cgcatccatg cccccgaact gcaggagtgg 3301 ggaggcacga tggccgcttt ggtcgacccg gacgggacgc tcctgcgcct gatacagaac 3361 gaattgcttg caggcatctc atgagtgtgt cttcccgttt tccgcctgag gtcactgcgt 3421 ggatggagcg ctggcgcctg ctgcgcgacg gcgagctgct caccacccac tcgccaagct 3481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 3541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 3601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 3661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 3721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 3781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 3841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 3901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 3961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 4021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 4081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 4141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 4201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 4261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 4321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 4381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 4441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 4501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 4561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 4621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 4681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 4741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 4801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 4861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 4921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 4981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 5041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 5101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 5161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 5221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg 5281 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 5341 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 5401 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 5461 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 5521 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 5581 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 5641 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 5701 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc 5761 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 5821 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 5881 gctgatctat acattgaatc aatattggca attagccata ttagtcat //