LOCUS Exported 3746 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C90m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3746) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 3746) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3746 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 44..62 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 66..109 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 112..133 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS 1073..1786 /codon_start=1 /product="GFP variant that folds robustly even when fused to poorly folded proteins (Pedelacq et al., 2006)" /label=superfolder GFP /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" misc_feature 1801..1821 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(1827..1844) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1998..2586) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2078..2097) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2757..3617) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3380..3399 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3618..3722) /gene="bla" /label=AmpR promoter ORIGIN 1 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 61 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg 121 gccgcttcta gagactagtg ggtctcaaat ggaactgcgt gacctggatt taaacctgct 181 ggtggtgttc aaccagttgc tggtcgacag acgcgtctct gtcactgcgg agaacctggg 241 cctgacccag cctgccgtga gcaatgcgct gaaacgcctg cgcacctcgc tacaggaccc 301 actcttcgtg cgcacacatc agggaatgga acccacaccc tatgccgcgc atctggccga 361 gcacgtcact tcggccatgc acgcactgcg caacgcccta cagcaccatg aaagcttcga 421 tccgctgacc agcgagcgta ccttcaccct ggccatgacc gacattggcg agatctactt 481 catgccgcgg ctgatggatg cgctggctca ccaggccccc aattgcgtga tcagtacggt 541 gcgcgacagt tcgatgagcc tgatgcaggc cttgcagaac ggaaccgtgg acttggccgt 601 gggcctgctt cccaatctgc aaactggctt ctttcagcgc cggctgctcc agaatcacta 661 cgtgtgccta tgtcgcaagg accatccagt cacccgcgaa cccctgactc tggagcgctt 721 ctgttcctac ggccacgtgc gtgtcatcgc cgctggcacc ggccacggcg aggtggacac 781 gtacatgaca cgggtcggca tccggcgcga catccgtctg gaagtgccgc acttcgccgc 841 cgttggccac atcctccagc gcaccgatct gctcgccact gtgccgatat gtttagccga 901 ctgctgcgta gagcccttcg gcctaagcgc cttgccgcac ccagtcgtct tgcctgaaat 961 agccatcaac atgttctggc atgcgaagta ccacaaggac ctagccaata tttggttgcg 1021 gcaactgatg tttgacctgt ttacggatgg aagcggaagt ggatccggta gtatgcgtaa 1081 aggcgaagag ctgttcactg gtgtcgtccc tattctggtg gaactggatg gtgatgtcaa 1141 cggtcataag ttttccgtgc gtggcgaggg tgaaggtgac gcaactaatg gtaaactgac 1201 gctgaagttc atctgtacta ctggtaaact gccggtacct tggccgactc tggtaacgac 1261 gctgacttat ggtgttcagt gctttgctcg ttatccggac catatgaagc agcatgactt 1321 cttcaagtcc gccatgccgg aaggctatgt gcaggaacgc acgatttcct ttaaggatga 1381 cggcacgtac aaaacgcgtg cggaagtgaa atttgaaggc gataccctgg taaaccgcat 1441 tgagctgaaa ggcattgact ttaaagaaga tggcaatatc ctgggccata agctggaata 1501 caattttaac agccacaatg tttacatcac cgccgataaa caaaaaaatg gcattaaagc 1561 gaattttaaa attcgccaca acgtggagga tggcagcgtg cagctggctg atcactacca 1621 gcaaaacact ccaatcggtg atggtcctgt tctgctgcca gacaatcact atctgagcac 1681 gcaaagcgtt ctgtctaaag atccgaacga gaaacgcgat catatggttc tgctggagtt 1741 cgtaaccgca gcgggcatca cgcatggtat ggatgaactg tacaaataaa ggtagagacc 1801 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 1861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 1921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 1981 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga 2041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 2101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 2161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 2221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 2281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 2341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 2401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 2461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 2521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 2581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 2641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 2701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 2761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 2821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 2881 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag 2941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 3001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 3061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 3121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 3181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 3241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 3301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 3361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 3421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 3481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 3541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 3601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 3661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 3721 cgcacatttc cccgaaaagt gccacc //