LOCUS Exported 2822 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file. ACCESSION . VERSION . KEYWORDS C91m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2822) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2822) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2822 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 27..45 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 49..92 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 95..116 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 860..880 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(886..903) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1057..1645) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1137..1156) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1816..2676) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2439..2458 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2677..2781) /gene="bla" /label=AmpR promoter ORIGIN 1 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa 61 aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta 121 gtgggtctca aatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg 181 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg 241 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt 301 ggccgactct ggtaacgacg ctgacttggg gtgttcagtg ctttgctcgt tatccggacc 361 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca 421 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg 481 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc 541 tgggccataa gctggaatac aattacatca gcgacaatgt ttacatcacc gccgataaac 601 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc 661 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag 721 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc 781 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt 841 acaaataaag gtagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc 901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 961 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 1021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga 1081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 1141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 1201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 1261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 1321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 1381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 1441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 1501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 1561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 1681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1801 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 1861 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 1921 cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga 1981 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 2041 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 2101 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 2161 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 2221 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 2281 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 2341 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 2401 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 2461 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 2521 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 2581 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 2641 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 2701 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 2761 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 2821 ca //