LOCUS Exported 2804 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).. ACCESSION . VERSION . KEYWORDS C99m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2804) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2804) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2804 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 33..51 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 55..98 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 101..122 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 848..868 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(874..891) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1045..1633) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1125..1144) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1804..2664) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2427..2446 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2665..2769) /gene="bla" /label=AmpR promoter ORIGIN 1 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca 61 gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag 121 agactagtgg gtctcaaatg gtcagtaaag gcgaagcagt tatcaaagag ttcatgcgct 181 tcaaagttca tatggaaggg tcgatgaacg ggcacgaatt tgaaattgaa ggcgaaggcg 241 aaggccgccc atatgaaggg acccaaaccg caaagcttaa ggttactaaa ggcggtccat 301 tacccttttc gtgggacatt ttaagcccac agtttatgta cgggagtcgc gctttcatca 361 agcaccctgc ggacatccca gattactaca aacagtcttt ccccgagggg ttcaagtggg 421 agcgcgtgat gaacttcgag gatggcggag ccgtgacggt cacccaagat acctctttgg 481 aggacggtac gttgatctac aaagtgaaat tgcgtggcac gaattttcca cctgatgggc 541 ctgtcatgca gaaaaagaca atgggatggg aagcttccac ggagcgcctt tacccagagg 601 acggtgttct taaaggggat atcaaaatgg cgctgcgtct taaagatgga ggccgctacc 661 tggcggactt caagactact tacaaggcca aaaaaccagt gcagatgccg ggtgcgtaca 721 atgtagatcg taaattagat attacaagtc acaatgaaga ttacacggtc gtagagcagt 781 atgagcgcag tgaggggcgt cactctacgg gcggtatgga cgagttatac aagtaaaggt 841 agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt 901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 1021 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa 1081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 1441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 1501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 1561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 1621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 1681 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1741 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1801 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 1861 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 1921 ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat 1981 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 2041 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2101 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2161 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2221 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 2281 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2341 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 2401 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 2461 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 2521 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 2581 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 2641 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 2701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 2761 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taag //