LOCUS       Exported                6373 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  An adeno-associated viral vector expressing HA-tagged stimulatory 
            DREADD (hM3D(Gq)) from a synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pOTTC1596 - pAAV SYN1 HA-hM3D(Gq)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Galvan A, Raper J, Hu X, Pare JF, Bonaventura J, Richie CT, 
            Michaelides M, Mueller SAL, Roseboom PH, Oler JA, Kalin NH, Hall RA,
            Smith Y
  TITLE     Ultrastructural localization of DREADDs in monkeys.
  JOURNAL   Eur J Neurosci. 2019 May 7. doi: 10.1111/ejn.14429.
  PUBMED    31063250
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             663..689
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2500..3088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2553..2573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2971..2982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3120..3596
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3319..3338)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3636..3776
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3636..3765
                     /label=AAV2 ITR
     rep_origin      3851..4306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3938..3957)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4148..4169
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4323..4342)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4442..4464
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4502..4520)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4588..4692
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4693..5553
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4911..4930)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5724..6312
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6213..6232
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgaagttatc gctagcacca
      661 tgtacccata cgatgttcca gattacgcta ccttgcacaa taacagtaca acctcgcctt
      721 tgtttccaaa catcagctcc tcctggatac acagcccctc cgatgcaggg ctgcccccgg
      781 gaaccgtcac tcatttcggc agctacaatg tttctcgagc agctggcaat ttctcctctc
      841 cagacggtac caccgatgac cctctgggag gtcataccgt ctggcaagtg gtcttcatcg
      901 ctttcttaac gggcatcctg gccttggtga ccatcatcgg caacatcctg gtaattgtgt
      961 catttaaggt caacaagcag ctgaagacgg tcaacaacta cttcctctta agcctggcct
     1021 gtgccgatct gattatcggg gtcatttcaa tgaatctgtt tacgacctac atcatcatga
     1081 atcgatgggc cttagggaac ttggcctgtg acctctggct tgccattgac tgcgtagcca
     1141 gcaatgcctc tgttatgaat cttctggtca tcagctttga cagatacttt tccatcacga
     1201 ggccgctcac gtaccgagcc aaacgaacaa caaagagagc cggtgtgatg atcggtctgg
     1261 cttgggtcat ctcctttgtc ctttgggctc ctgccatctt gttctggcaa tactttgttg
     1321 gaaagagaac tgtgcctccg ggagagtgct tcattcagtt cctcagtgag cccaccatta
     1381 cttttggcac agccatcgct ggtttttata tgcctgtcac cattatgact attttatact
     1441 ggaggatcta taaggaaact gaaaagcgta ccaaagagct tgctggcctg caagcctctg
     1501 ggacagaggc agagacagaa aactttgtcc accccacggg cagttctcga agctgcagca
     1561 gttacgaact tcaacagcaa agcatgaaac gctccaacag gaggaagtat ggccgctgcc
     1621 acttctggtt cacaaccaag agctggaaac ccagctccga gcagatggac caagaccaca
     1681 gcagcagtga cagttggaac aacaatgatg ctgctgcctc cctggagaac tccgcctcct
     1741 ccgacgagga ggacattggc tccgagacga gagccatcta ctccatcgtg ctcaagcttc
     1801 cgggtcacag caccatcctc aactccacca agttaccctc atcggacaac ctgcaggtgc
     1861 ctgaggagga gctggggatg gtggacttgg agaggaaagc cgacaagctg caggcccaga
     1921 agagcgtgga cgatggaggc agttttccaa aaagcttctc caagcttccc atccagctag
     1981 agtcagccgt ggacacagct aagacttctg acgtcaactc ctcagtgggt aagagcacgg
     2041 ccactctacc tctgtccttc aaggaagcca ctctggccaa gaggtttgct ctgaagacca
     2101 gaagtcagat cactaagcgg aaaaggatgt ccctggtcaa ggagaagaaa gcggcccaga
     2161 ccctcagtgc gatcttgctt gccttcatca tcacttggac cccatacaac atcatggttc
     2221 tggtgaacac cttttgtgac agctgcatac ccaaaacctt ttggaatctg ggctactggc
     2281 tgtgctacat caacagcacc gtgaaccccg tgtgctatgc tctgtgcaac aaaacattca
     2341 gaaccacttt caagatgctg ctgctgtgcc agtgtgacaa aaaaaagagg cgcaagcagc
     2401 agtaccagca gagacagtcg gtcatttttc acaagcgcgc acccgagcag gccttgtagg
     2461 gcgcgccata acttcgaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
     2521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2881 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
     2941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3061 cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga gagatctacg
     3121 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     3181 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     3241 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     3301 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     3361 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     3421 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     3481 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     3541 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     3601 ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     3661 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3721 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     3781 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     3841 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3901 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     3961 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4021 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4081 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4141 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     4201 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     4261 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4321 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4381 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4441 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4501 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4561 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4621 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4681 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4741 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4801 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4861 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4921 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4981 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5041 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5101 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5161 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5221 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5281 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5341 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5401 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5461 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5521 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5581 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5641 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5701 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5761 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5821 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5881 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5941 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6001 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6061 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6121 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6181 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6241 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6301 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6361 tttgctcaca tgt
//
