LOCUS       Exported                3092 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  MoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family 
            repressor chimera with LAA degradation tag for ssrA; see DOI: 
            10.1021/sb500262f)..
ACCESSION   .
VERSION     .
KEYWORDS    C102m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3092)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     21..39
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      43..86
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    89..110
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(148..167)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     misc_feature    1124..1144
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1150..1167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1321..1909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1401..1420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2080..2940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2703..2722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2941..3045)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
       61 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt
      121 ctcaaatgat gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctctacgg
      181 cgaccgtttc caacgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
      241 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgccgcacaa caactggcgg
      301 gcaaacagag caaagtggtc gccatcattg ttacccgtct ggattcgttg tcagaaaatc
      361 tcgccgttca aaccatgctg ccagcgttct atgaacaagg ttacgaccca atcatgatgg
      421 aaagtcagtt ttccccgcaa ttagttgccg aacatttggg agtgctgaaa cggcgtaata
      481 tcgacggcgt agtgctgttc ggttttactg gcataacaga agaaatgtta gcccactggc
      541 agtcatcgct ggttctgctg gcgcgtgacg caaaaggctt tgcttcggtc tgttatgacg
      601 acgaaggggc aatcaaaatc ctgatgcaac ggctgtatga ccaggggcat cgtaatatca
      661 gttatctcgg cgtgccgcac agtgacgtga caaccggtaa gcgacgtcac gaagcctacc
      721 tggcgttctg caaagcgcat aaactgcatc ccgttgccgc cctgccaggg cttgctatga
      781 agcaaggcta tgagaacgtt gcaaaagtga ttacgcctga aactaccgcc ttactgtgcg
      841 caaccgacac gctggcactt ggcgcaagta aatacctgca agagcaacgc atcgacacct
      901 tgcaactggc gagcgtcggt aatacgccgt taatgaaatt cctccatccg gagatcgtaa
      961 ccgtagatcc cggttacgcc gaagctggac gccaggcggc ttgccagttg atcgcgcagg
     1021 taaccgggcg cagcgaaccg caacaaatca tcatccccgc caccctgtcc actagtgcag
     1081 cgaacgacga aaattacgcc cttgcagcgt gaaggtagag acctactagt agcggccgct
     1141 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1321 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1741 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2221 cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3061 agtgccacct gacgtctaag aaaccattat ta
//
