LOCUS       Exported               10707 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian lenti vector for expression of the 40nm GEM and DSRed.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-PfV-Sapphire-IRES-DSRed
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Delarue M, Brittingham GP, Pfeffer S, Surovtsev IV, Pinglay S, 
            Kennedy KJ, Schaffer M, Gutierrez JI, Sang D, Poterewicz G, Chung 
            JK, Plitzko JM, Groves JT, Jacobs-Wagner C, Engel BD, Holt LJ
  TITLE     mTORC1 Controls Phase Separation and the Biophysical Properties of 
            the Cytoplasm by Tuning Crowding.
  JOURNAL   Cell. 2018 Jul 12;174(2):338-349.e20. doi: 
            10.1016/j.cell.2018.05.042. Epub 2018 Jun 21.
  PUBMED    29937223
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3925..4641
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSIQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     misc_feature    4658..5230
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4681..5232
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4825..4842)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5232..6662
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    6676..7264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6729..6749)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7147..7158)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7471..8104
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8232..8248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8232..8248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8245..8267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8256..8272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8280..8310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8325..8346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8463..8480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8634..9222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8714..8733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9393..10253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10016..10035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10254..10358)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10406..10540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10443..10462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10497..10516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
     2821 tctagaatgc tctcaataaa tccaaccctt ataaataggg ataagccata caccaaagaa
     2881 gaattaatgg aaattctgag attagcaata atagctgagc ttgatgcaat aaatctatat
     2941 gagcagatgg caagatattc cgaagatgaa aacgtaagaa agattctcct tgatgttgca
     3001 agggaggaga aggctcacgt tggagaattc atggcactcc ttctaaattt ggatccagaa
     3061 caagtaactg agctgaaggg tggatttgag gaagtcaagg agctaactgg aattgaggct
     3121 cacataaatg ataacaagaa ggaggaatct aacgtggaat attttgagaa gttgagaagt
     3181 gctcttttgg atggggtaaa taagggaaga tctttactaa agcacttgcc agtaacgaga
     3241 atagaaggac aatcattcag ggttgacatc attaaatttg aagatggtgt tagggtagta
     3301 aagcaagagt acaagccaat tccattattg aagaagaaat tctatgtggg aataagagaa
     3361 ctcaacgatg ggacttacga tgtatctata gcaactaaag ctggggaact cctagttaaa
     3421 gacgaagagt cgctagtaat aagagaaatt ctatccacag agggaattaa gaaaatgaaa
     3481 ctttcctcct gggacaatcc agaagaagca cttaatgatt taatgaacgc actgcaggaa
     3541 gcctcaaatg cgtcagctgg accatttggg ctgataatta acccaaaaag atatgcaaag
     3601 ttgctaaaaa tttacgagaa gagtggaaag atgctagttg aagtgctaaa agagatattc
     3661 aggggaggaa tcattgttac tctaaacatt gatgagaata aggtaataat atttgccaat
     3721 acaccagctg ttcttgacgt tgtagttggc caagatgtga ctctacaaga gctaggacct
     3781 gaaggagacg acgttgcatt cttggttagt gaagctatag gaataagaat aaagaaccca
     3841 gaggcaatag tagtcttaga gtggtctggc agcggtagtg ggtcgggatc aggctccggt
     3901 cgacggatcc ccgggttaat taagatgtct aaaggtgaag aattattcac tggtgttgtc
     3961 ccaattttgg ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa
     4021 ggtgaaggtg atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa
     4081 ttgccagttc catggccaac cttagtcact actttttctt atggtgttca atgttttgct
     4141 agatacccag atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat
     4201 gttcaagaaa gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc
     4261 aagtttgaag gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa
     4321 gatggtaaca ttttaggtca caaattggaa tacaacttta actctcacaa tgtttacatc
     4381 atggctgaca aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa
     4441 gatggttctg ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca
     4501 gtcttgttac cagacaacca ttacttatcc attcaatctg ccttatccaa agatccaaac
     4561 gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt
     4621 attgatgaat tgtacaaatg agcggccgcg gatcccgccc ctctccctcc ccccccccta
     4681 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     4741 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     4801 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     4861 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     4921 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     4981 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5041 aaagagtcaa atggctcacc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     5101 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     5161 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     5221 acgatgataa tatggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
     5281 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     5341 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     5401 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     5461 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     5521 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     5581 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     5641 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     5701 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     5761 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     5821 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     5881 gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc ggcagcggca
     5941 gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag ttcatgcgct
     6001 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     6061 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     6121 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
     6181 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
     6241 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
     6301 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
     6361 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
     6421 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc
     6481 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
     6541 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     6601 acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt
     6661 gaacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6721 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6781 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6841 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6901 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6961 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7021 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     7081 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7141 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7201 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7261 ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca
     7321 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7381 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7441 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     7501 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7561 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7621 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7681 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7741 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7801 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7861 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     7921 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7981 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8041 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8101 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8161 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     8221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8281 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     9121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10381 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10501 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10561 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10621 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10681 ttaaatatgt actacaaact tagtagt
//
