LOCUS Exported 2192 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).. ACCESSION . VERSION . KEYWORDS U3m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2192) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2192) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2192 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 29..47 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 51..94 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 97..118 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 232..252 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(258..275) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(429..1017) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(509..528) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1188..2048) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1811..1830 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2049..2153) /gene="bla" /label=AmpR promoter ORIGIN 1 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata 61 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac 121 tagtgggtct catactgggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt 181 ttcgtactga aacatcttaa tcatgcaatg gaggctttct aatgagagac ctactagtag 241 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 421 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 841 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 1021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 1081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 1141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 1201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 1261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 1321 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga 1381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 1441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 1501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 1561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 1621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 1681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 1741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 1801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 1861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 1921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 1981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 2041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 2101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 2161 ccccgaaaag tgccacctga cgtctaagaa ac //