LOCUS Exported 2192 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).. ACCESSION . VERSION . KEYWORDS U7m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2192) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2192) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2192 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 17..35 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 39..82 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 85..106 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 220..240 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(246..263) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(417..1005) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(497..516) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1176..2036) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1799..1818 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2037..2141) /gene="bla" /label=AmpR promoter ORIGIN 1 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt 61 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca 121 tactgggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa 181 catcttaatc atgcttagga gtctttctaa tgagagacct actagtagcg gccgctgcag 241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 421 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac 1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 2161 ccacctgacg tctaagaaac cattattatc at //