LOCUS       Exported                1138 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    pET21a-MTF-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1022..1039
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1092..1110)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tttaggggaa tgkgagcgat aacaattccc tctagaaata atttgtttaa ctttaagaag
       61 gagatataca tatgtcagaa tcactacgta ttattttgcg ggtacacctg actttgcagc
      121 gcgtcatctc gacgcgctgt tgtcttctgg tcataacgtc gttggcgtgt tcacccagcc
      181 agaccgaccg gcaggacgcg gtaaaaaact gatgcccagc ccggttaaag ttctggctga
      241 ggaaaaaggt ctgcccgttt ttcaacctgt ttccctgcgt ccacaagaaa accagcaact
      301 ggtcgccgaa ctgcaggctg atgttatggt cgtcgtcgcc tatggtttaa ttctgccgaa
      361 agcagtgctg gagatgccgc gtcttggctg tatcaacgtt catggttcac tgctgccacg
      421 ctggcgcggt gctgcaccaa tccaacgctc actatgggcg ggtgatgcag aaactggtgt
      481 gaccattatg caaatggatg tcggtttaga caccggtgat atgctctata agctctcctg
      541 cccgattact gcagaagata ccagtggtac gctgtacgac aagctggcag agcttggccc
      601 acaagggctt atcaccacgt tgaaacaact ggcagacggc acggcgaaac cagaagttca
      661 ggacgaaact cttgtcactt acgccgagaa gttgagtaaa gaagaagcgc gtattgactg
      721 gtcactttcg gcagcacagc ttgaacgctg cattcgcgct ttcaatccat ggccaatgag
      781 ctggctggaa attgaaggac agccggttaa agtctggaaa gcatcggtca ttgatacggc
      841 aaccaacgct gcaccaggaa cgatccttga agccaacaaa caaggcattc aggttgcgac
      901 tggtgatggc atcctgaacc tgctctcgtt acaacctgcg ggtaagaaag cgatgagcgc
      961 gcaagacctc ctgaactctc gtcgggaatg gtttgttccg ggcaaccgtc tggtcctcga
     1021 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     1081 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtc
//
