LOCUS       Exported                2166 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  MoClo golden gate assembly DE part for L3S2P21 (382x-strength 
            synthetic terminator with BsaI cut site removed; see DOI: 
            10.1038/nmeth.2515)..
ACCESSION   .
VERSION     .
KEYWORDS    T18m
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2166)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      39..82
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    85..106
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    194..214
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(220..237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(391..979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(471..490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1150..2010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1773..1792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2011..2115)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
       61 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca
      121 aggtggccaa ttattgaagg cctccctaac ggggggcctt tttttgtttc tggtctgccc
      181 tcgcttagag acctactagt agcggccgct gcaggcttcc tcgctcactg actcgctgcg
      241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
      301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
      421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
      601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     1141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1261 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
     1321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1921 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     2161 tatcat
//
