LOCUS Exported 2164 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly AB part for pRpa* (improved pRpa from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS P37m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2164) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2164) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2164 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 16..34 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 38..81 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 84..105 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 191..211 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(217..234) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(388..976) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(468..487) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1147..2007) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1770..1789 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2008..2112) /gene="bla" /label=AmpR promoter ORIGIN 1 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta 61 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctcag 121 gaggcacctg tccgatcgga cagtattacg caagaaaatg gtttgttata gtcgaatatt 181 actagagacc tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc 241 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 301 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 361 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca 421 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 481 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 541 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 601 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 661 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 721 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 781 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 841 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 901 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 961 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 1021 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 1081 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1141 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 1201 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 1261 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc 1321 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 1381 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 1441 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 1501 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 1561 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 1621 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 1681 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 1741 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 1801 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 1861 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 1921 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 1981 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 2041 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 2101 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 2161 catg //