LOCUS Exported 2831 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C32m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2831) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2831) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2831 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 28..46 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 50..93 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 96..117 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 870..890 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(896..913) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1067..1655) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1147..1166) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1826..2686) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2449..2468 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2687..2791) /gene="bla" /label=AmpR promoter ORIGIN 1 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 61 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact 121 agtgggtctc aaatgattga gaatacctat agcgaaaagt tcgagtccgc gttcgaacag 181 atcaaggcgg cggccaacgt ggatgccgcc atccgtattc tccaggcgga atataacctc 241 gatttcgtca cctaccatct cgcccagacg atcgcgagca agatcgatcc gcccttcgtg 301 cgcaccacct atccggatgc ctgggtttcc cgctacctcc tcaacagcta tgtgaaggtc 361 gatccgatcg tcaagcaggg cttcgaacgc cagctgccct tcgactggag cgaggtcgaa 421 ccgacgccgg aggcctatgc catgctggtc gacgcccaga aacacggcat cggtggcaat 481 ggctactcca tccccgtcgc cgacaaggcg cagcgccgcg ccctgctgtc gctgaatgcc 541 cgtataccgg ccgacgaatg gaccgagctt gtgcgccgct gccgcaacga gtggatcgag 601 atcgcccatc tgatccaccg caaggccgtc tatgagctgc atggcgaaaa cgatccggtg 661 ccggcattgt cgccgcgcga gatcgagtgt ctgcactgga ccgccctcgg caaggattac 721 aaggatattt cggtcatcct gggcatatca gagcatacca cacgcgatta cctgaagacc 781 gcccgcttca agctcggctg cgccacgatc tcggccgccg cgtcgcgggc tgttcaattg 841 cgcatcatca atccctgaag gtagagacct actagtagcg gccgctgcag gcttcctcgc 901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc 1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 1501 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac ccacgctcac 1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 2821 tctaagaaac c //