LOCUS Exported 3606 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.. ACCESSION . VERSION . KEYWORDS C31m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3606) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 3606) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3606 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 28..46 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 50..93 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 96..117 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" primer_bind complement(1671..1688) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1842..2430) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1922..1941) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2601..3461) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3224..3243 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3462..3566) /gene="bla" /label=AmpR promoter ORIGIN 1 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 61 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact 121 aggtctcaaa tggtgagagc cctggtagtc atccgcctgt cccgcgtcac cgatgctacg 181 acttcaccgg agcgtcagct ggagtcttgc cagcagctct gcgcccagcg cggctgggac 241 gtcgtcgggg tagcggagga tctggacgtc tccggggcgg tcgatccgtt cgaccggaag 301 cgcagaccga acctggcccg gtggctagcg ttcgaggagc aaccgtttga cgtgatcgtg 361 gcgtaccggg tagatcggtt gacccgatcg atccggcatc ttcagcagct ggtccactgg 421 gccgaggacc acaagaagct ggtcgtctcc gcgaccgaag cgcacttcga tacgacgacg 481 ccgtttgcgg cggtcgtcat cgcgcttatg ggaacggtgg cgcagatgga attagaagcg 541 atcaaagagc ggaaccgttc ggctgcgcat ttcaatatcc gcgccgggaa ataccgagga 601 tccctgccgc cgtggggata cctgcctacg cgcgtggacg gggagtggcg gctggtgccg 661 gaccctgtgc agcgagagcg catcctcgag gtgtatcacc gcgtcgtcga caaccacgag 721 ccgctgcatc tggtggccca cgacctgaac cggcgtggtg tcctgtcgcc gaaggactac 781 ttcgcgcagc tgcaaggccg cgagccgcag ggccgggagt ggtcggctac cgcgctgaag 841 cgatcgatga tctccggggc gatgctcggg tacgcgactc tgaacggtaa gaccgtccga 901 gacgacgacg gagccccgct ggtgcgggct gagccgatcc tgacccgtga gcagctggag 961 gcgctgcgcg ccgagctcgt caagacctcc cgggcgaagc ccgcggtgtc taccccgtcg 1021 ctgctgctgc gggtgttgtt ctgcgcggtg tgcggggagc ccgcgtacaa gttcgccggg 1081 ggaggacgta agcacccgcg ctaccgctgc cgctcgatgg ggttcccgaa gcactgcggg 1141 aacggcacgg tggcgatggc cgagtgggac gcgttctgcg aggagcaggt actggatctg 1201 ctcggggacg cggagcgtct ggagaaagtc tgggtagcgg gctcggactc cgcggtcgaa 1261 ctcgcggagg tgaacgcgga gctggtggac ctgacgtcgc tgatcggctc cccggcctac 1321 cgggcgggct ctccgcagcg agaagcactg gatgcccgta ttgcggcgct ggccgcgcgg 1381 caagaggagc tggagggcct ggaggctcgc ccgtctggct gggagtggcg cgaaaccggg 1441 cagcggttcg gggactggtg gcgggagcag gacaccgcgg caaagaacac ctggcttcgg 1501 tcgatgaacg ttcggctgac gttcgacgtc cgcggcgggc tgactcgcac gatcgacttc 1561 ggggatcttc aggagtacga gcagcatctc aggctcggca gcgtggtcga acggctacac 1621 accgggatgt cgtaaaggtt gagaccctag tagcggccgc tgcaggcttc ctcgctcact 1681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 1741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 1801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc 1861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 1921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 1981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 2041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 2281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 2341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 2401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 2461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 2521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 2581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 2641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 2701 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct 2761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 2821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 2881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 2941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 3001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 3061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 3181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 3241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 3301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 3361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 3421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 3481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 3541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 3601 gaaacc //