LOCUS Exported 3321 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly. ACCESSION . VERSION . KEYWORDS HB401: pMVP (L1-L4) CMV promoter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3321) AUTHORS Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB TITLE Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. JOURNAL Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PUBMED 30590691 REFERENCE 2 (bases 1 to 3321) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3321 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" enhancer 824..1203 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1204..1407 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1358..1378 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 1404..1428 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind complement(1436..1531) /gene="mutant version of attL" /label=attL4 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1562..1581) /label=T7 /note="T7 promoter, forward primer" promoter complement(1563..1581) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1586..1602) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1586..1602) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1704..1723) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1715..2524 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1782..1801) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2671..3259 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3160..3179 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctta cgatctatac attgaatcaa tattggcaat tagccatatt agtcattggt 721 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat 781 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact 841 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 901 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 961 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 1021 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 1081 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 1141 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 1201 atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga 1261 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1321 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta 1381 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatccg gcacccaact 1441 tttctataca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag 1501 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagggcg gccgcgatat 1561 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 1621 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 1681 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 1741 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 1801 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 1861 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 1921 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 1981 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 2041 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 2101 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 2161 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 2221 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 2281 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 2341 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 2401 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 2461 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 2521 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 2581 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 2641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 2701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 2761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 2821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 2881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 2941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 3001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 3061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 3121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 3181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 3241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 3301 ctggcctttt gctcacatgt t //