LOCUS Exported 3021 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION pMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assembly. ACCESSION . VERSION . KEYWORDS LF801: pMVP (L1-R5) cTnT promoter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3021) AUTHORS Haldeman JM, Conway AE, Arlotto ME, Slentz DH, Muoio DM, Becker TC, Newgard CB TITLE Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. JOURNAL Nucleic Acids Res. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. PUBMED 30590691 REFERENCE 2 (bases 1 to 3021) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3021 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind 1084..1207 /gene="mutant version of attR" /label=attR5 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1262..1281) /label=T7 /note="T7 promoter, forward primer" promoter complement(1263..1281) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1286..1302) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1286..1302) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1404..1423) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1415..2224 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(1482..1501) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2371..2959 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2860..2879 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctgc agtctgggct ttcacaagac agcatctggg gctgcggcag agggtcgggt 721 ccgaagcgct gccttatcag cgtccccagc cctgggaggt gacagctggc tggcttgtgt 781 cagcccctcg ggcactcacg tatctccgtc cgacgggttt aaaatagcaa aactctgagg 841 ccacacaata gcttgggctt atatgggctc ctgtggggga agggggagca cggagggggc 901 cggggccgct gctgccaaaa tagcagctca caagtgttgc attcctctct gggcgccggg 961 cacattcctg ctggctctgc ccgccccggg gtgggcgccg gggggacctt aaagcctctg 1021 ccccccaagg agcccttccc agacagccgc cggcacccac cgctccgtgg gacctaagct 1081 tgccaacttt gtatacaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat 1141 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt 1201 cactatgaat caactactta gatggtatta gtgacctgta ctgcagggcg gccgcgatat 1261 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 1321 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 1381 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 1441 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 1501 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 1561 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 1621 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 1681 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 1741 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 1801 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 1861 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 1921 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 1981 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 2041 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 2101 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 2161 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 2221 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 2281 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 2341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 2401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 2461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 2521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 2581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 2641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 2701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 2761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 2821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 3001 ctggcctttt gctcacatgt t //