LOCUS Exported 3233 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 0 Part. Insertion site. ACCESSION . VERSION . KEYWORDS pC0.281 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3233) AUTHORS Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick AJ TITLE CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax. JOURNAL Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub 2019 Feb 28. PUBMED 30819783 REFERENCE 2 (bases 1 to 3233) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3233 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(36..54) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 122..226 /gene="bla" /label=AmpR promoter CDS 448..1239 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1410..1998 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1899..1918 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2152..2169 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata 61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa 241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc 301 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg 361 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc 421 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact 481 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta 541 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg 601 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg 661 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt 721 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa 781 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg 841 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag 901 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg 961 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc 1021 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg 1081 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat 1141 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat 1201 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca 1261 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 1321 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 1801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 1981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 2041 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 2101 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 2161 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 2221 attcattaat cactctgtgg tctcaggagg gccatcgttt tattaacggt gttctgcgcc 2281 gggttagcga tcgcctccgg gctgaggaca gcctcaaata gatgccctct aggtcattat 2341 tatcttaaga aactcgtccc taagccaaaa acaatactgg tgagcactaa taggagatgt 2401 tcgggggcga tcgccacggt gacgccagtg ctattgagga tggcattcac accactaccg 2461 agggcaatgc ctgcgaccag ggcaacaatc ccaatcagag cggctcgccc aaagcgttgt 2521 tctttacggt agagcaaata aatattcgcg atcaatgcca cagtcaaaag agttgaggtg 2581 agatccgttg cgaggaatat tccggcaatg gcgatcgccc cattgacacc gagggcagta 2641 tatagagtgt tggtttctgg tgtatcccgt aaattgacca accactgggg cagttcggga 2701 gacttcacca acggttgaga cgcctgcgcc ttcttctgag aagtttctgc aaagcgaatt 2761 ttatccggaa catcgagggt gccctgttgt cgttgtctaa ggcggtccat aataatcgca 2821 tcgtaggccg cttcgagttt gtccactacg gcgcgatcgc cttcgtattg ggcgcttagt 2881 ttttgtttgg ctgcttggat ctcttcaaat gaagccgttt ttgcaagccc aagggtttcg 2941 tagggatttt gttcgctcat tctcctcctc ctgtgagcat gccactgggt tattggaaca 3001 tccttagatt cttaatgtaa cccaatcatc atacagttta ttattatgag cgttttccgg 3061 tgcgctttcc atagagcaaa attttttctg gtgatgatag ccggggcgat cgcctcaaga 3121 cttgcccaga ccagagagta tacatcccca tacttaacgc tagctgatca acggagtagg 3181 tactcgaacc actcaggctc cctaaaccgc tgcaatgtac aaaatcttaa atg //