LOCUS Exported 5897 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation. ACCESSION . VERSION . KEYWORDS pMX-eSOX17WHC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5897) AUTHORS Veerapandian V, Ackermann JO, Srivastava Y, Malik V, Weng M, Yang X, Jauch R TITLE Directed Evolution of Reprogramming Factors by Cell Selection and Sequencing. JOURNAL Stem Cell Reports. 2018 Aug 14;11(2):593-606. doi: 10.1016/j.stemcr.2018.07.002. Epub 2018 Aug 2. PUBMED 30078555 REFERENCE 2 (bases 1 to 5897) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5897 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1094..1510 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1440..1462 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1478..1494 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1520..1894 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1832..1848 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" protein_bind 1915..1939 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3205..3229) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" LTR 3402..3871 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4001..4018) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4172..4760) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4252..4271) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4924..5784) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5547..5566 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5785..5889) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aatcacaagt 1921 ttgtacaaaa aagcaggctt caccatgagc agcccggatg cgggatacgc cagtgacgac 1981 cagagccagc cccggagcgc gcagcccgcg gtgatggcag ggttgggccc ctgtccctgg 2041 gccgagtccc tgagccccct cggggatgta aaggtgaaag gcgaggtggt ggcgagtagc 2101 ggggcgccag ccgggacgtc gggccgagcc aaagcggagt ctcgcatccg gcggccgatg 2161 aacgccttta tggtgtgggc caaagacgaa cgcaagcggt tggcacagca gaacccagat 2221 ctgcacaacg cagagctaag caagatgcta ggcaagtctt ggaaggcgtt gacctgggca 2281 gagaagcggc ccttccatga agaggcctgt cggctgcgcg tgcagcatat gcaggaccac 2341 cccaactaca agtaccggcc gcggcggcgc aagcaggtga agcgcatgaa gcgggtggag 2401 ggaggcttcc tgcacgctct cgtcgagccc caggccggcg cgcttggtcc cgagggcggc 2461 cgcgtggcca tggatggcct gggtctgcct ttcccggagc cgggctatcc ggccggtcct 2521 ccgctgatgt ctccgcacat gggcccccac tatcgggact gccagggact gggcgctccc 2581 gcgctcgacg gctaccctct gcccactccg gacacatccc cgctggatgg cgtggagcag 2641 gacccggctt tctttgcagc cccgctgcca ggggactgcc cggcggccgg cacctacact 2701 tacgctccag tctcggacta tgcagtgtcc gtagagccgc ccgctggccc catgcgagtg 2761 gggccggacc cctcgggccc tgcgatgccg gggatcctgg cgccccccag cgctctgcac 2821 ctgtactacg gcgcgatggg ctcgcccgcc gcaagtgcgg ggcgcggttt ccacgcgcaa 2881 ccccagcagc cgctgcaacc gcaggcaccg cccccgccac cgcagcagca gcacccagcg 2941 cacggccccg ggcaaccttc gccccctccc gaggctctgc cctgccggga tggcacggaa 3001 tccaaccagc ccactgagct cctaggggag gtggaccgca cggaattcga acagtatctg 3061 ccctttgtgt ataagcccga gatgggtctt ccctaccagg gacacgactg cggagtgaac 3121 ctctcagaca gccacggagc catttcctcc gtggtgtccg acgctagctc agcggtctac 3181 tattgcaact accccgacat ttaaacccag ctttcttgta caaagtggtg attcgacgat 3241 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag 3301 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag 3361 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag 3421 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga 3481 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac 3541 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc 3601 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc 3661 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct 3721 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa 3781 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag 3841 tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt 3901 tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg 3961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 4021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 4081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 4141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 4201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 4261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 4321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 4381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 4441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 4501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 4561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 4621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 4681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 4741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 4801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 4861 agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga 4921 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 4981 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 5041 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 5101 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 5161 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 5221 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 5281 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 5341 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 5401 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 5461 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 5521 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 5581 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 5641 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 5701 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 5761 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 5821 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 5881 ggttccgcgc acatttc //