LOCUS       Exported                3253 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Level 0 Part. Insertion site.
ACCESSION   .
VERSION     .
KEYWORDS    pC0.282
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(36..54)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        122..226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             448..1239
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1410..1998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1899..1918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2152..2169
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
       61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
      361 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
      421 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
      481 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      541 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
      601 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      661 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
      721 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
      781 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
      841 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
      901 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
      961 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     1021 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1081 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1141 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     1201 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     1261 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1321 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2041 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2101 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2161 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2221 attcattaat cactctgtgg tctcagctta tcccaaagac cctccactaa gcgatcgtgt
     2281 agaggccact gaacagacaa gcacaaaaat acaatcctcc cagactatgg tttgtgtccg
     2341 ttgatcgcac tcggcatgaa aataagtcca ttttgtcgat tgaccctacc ataaaaactc
     2401 aagcttgatc ttttatggat cctctttctt tcgatctggc tttgtaaaca taagtttatg
     2461 ttaattagtt aaagatgccc tgaattttat cagtttcatg aagcttcttt taacctcaga
     2521 ttaattaggc actaatttta tagaaaaaag cttcattata tagaatttct aaaaatcaag
     2581 ctttaaaggg ttgaccttcc ttttgaaagg taataaaact gaagtacaaa caaatacgta
     2641 agtaacgaaa aggagtaagc cgcaatctgc ctcagtagaa acacgtattt ttaccccaaa
     2701 agaagtattc tattcatctt tctcctttgt gagaaggttc atggagatcc ttctggggca
     2761 cttgaaacct ctacttgcaa ggatcaaagc gacatatgta cgtgtttttc caaaaaaatc
     2821 ccagaagtag ggagtaattc ctctactgag atttcaagaa aattaagtat tctgaggctt
     2881 ggtttatacg ggaaatttag acttgatggt ttgggtctta ccttttccta gcagcaagac
     2941 taaattaatt agttacgcat ttgttgaaat caaactgaat tctattgagg attccttaca
     3001 atggctaaat tcaattcttc tacaatcaca ttcgcaacca tcatcgcttt tgctgccatc
     3061 ggtcaagccg cacaggctgg cgaaagctac gtccgcaaca cattcgaaca cagcacttcc
     3121 aaaacgactt ctaaccttga tctggaaagc aaagattggt atcacggctc tcgtgaatgg
     3181 tctaactatg cttacaaaga atactacgat ggaactgtaa caaccagtgt agatggtgaa
     3241 ctctctagtc gct
//
