LOCUS       Exported                4335 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pVS1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Vodala S, Abruzzi KC, Rosbash M
  TITLE     The nuclear exosome and adenylation regulate posttranscriptional 
            tethering of yeast GAL genes to the nuclear periphery.
  JOURNAL   Mol Cell. 2008 Jul 11;31(1):104-13.
  PUBMED    18614049
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    2..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(139..156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(310..898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(390..409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1069..1929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1692..1711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1930..2034)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2065..2568
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2624..2642
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2644..2745
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             2746..3420
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(3539..3561)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3661..3680
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3874..3896
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3888..3905
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3889..3905
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3956..3972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3992..4008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4028..4044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4064..4080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4100..4116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4136..4152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4172..4188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4243..4259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4243..4259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4256..4278)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4267..4283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4291..4321)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
       61 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      121 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
      181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
      241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     1141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     2041 tccccgaaaa gtgccacctg gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct
     2101 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt
     2161 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa
     2221 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga
     2281 aaagcagatt aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat
     2341 tttcgtgtgt ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc
     2401 aggaagagca agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt
     2461 cggaaaacaa aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata
     2521 tatttatatt aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca
     2581 ggtggcacta agaaaccata ttatcatgac attaacctat aaaaataggc gtatcacgag
     2641 gccaattcgg tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg
     2701 agcacgtgag tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat
     2761 ttcacaggta gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca
     2821 gaatgtgcac tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag
     2881 agaacaattg acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat
     2941 agttcaggca ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt
     3001 ttggctctgg tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg
     3061 caagaatacc aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac
     3121 tgcaacatac tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat
     3181 tcagaagcag gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga
     3241 aggcaagaga gccccgagag cttacatttt atgttagctg gtggactgac gccagaaaat
     3301 gttggtgatg cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag
     3361 acaaatggtg taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag
     3421 gttattactg agtagtattt atttaagtat tgtttgtgca cttgcctgca agccttttga
     3481 aaagcaagca taaaagatcg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     3541 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     3601 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     3661 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     3721 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     3781 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3841 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3901 ccagtgaatt cgagctcggt acccggggat ccctcgagga attccacatg tggaattgtg
     3961 agcggataac aatttgtggc cacatgtgga attgtgagcg gataacaatt tgtggccaca
     4021 tgtggaattg tgagcggata acaatttgtg gccacatgtg gaattgtgag cggataacaa
     4081 tttgtggcca catgtggaat tgtgagcgga taacaatttg tggccacatg tggaattgtg
     4141 agcggataac aatttgtggc cacatgtgga attgtgagcg gataacaatt tgtggaattc
     4201 ggcgcctcta gagtcgacgc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     4261 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4321 agcctggggt gccta
//
