LOCUS Exported 10284 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION EF1a-ZIKV-protease-mTagBFP2. ACCESSION . VERSION . KEYWORDS pCF709 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10284) AUTHORS Oakes BL, Fellmann C, Rishi H, Taylor KL, Ren SM, Nadler DC, Yokoo R, Arkin AP, Doudna JA, Savage DF TITLE CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. JOURNAL Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052. PUBMED 30633905 REFERENCE 2 (bases 1 to 10284) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10284 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 232..611 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 613..811 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 766..786 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 829..1009 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1056..1181 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1674..1907 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2092..2136 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2434..2551 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2626..3803 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2857..3794 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3751..3771 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 3810..3819 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4539..4586 /codon_start=1 /product="two tandem FLAG(R) epitope tags" /label=2xFLAG /translation="DYKDDDDKDYKDDDDK" CDS 4608..4664 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" misc_feature 5376..5964 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5429..5449) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5847..5858) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6036..6269 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(6295..6312) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6301..6525 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6571..6999 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6658..6677) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6868..6889 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7008..7028) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7013..7342 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7193..7328 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7255..7274 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7390..7437 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7456..7830 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7960..8081 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7997..8016) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8051..8070 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8130..8146) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8130..8146) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8143..8165) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8154..8170 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8178..8208) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8223..8244 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8361..8378) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8532..9120) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8612..8631) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9291..10151) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9914..9933 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10152..10256) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat tgacattgat 241 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 301 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 361 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 421 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 481 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 541 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 601 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 661 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 721 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 781 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg 841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 1021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1501 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1561 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1621 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1681 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1741 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1801 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1861 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1921 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1981 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 2041 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 2101 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 2161 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 2221 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 2281 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 2341 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca 2401 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg 2461 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt 2521 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc 2581 agtttggtta attaagctag ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt 2641 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat 2701 tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg 2761 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac 2821 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc 2881 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cactggctgc 2941 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt 3001 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc 3061 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag 3121 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa 3181 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg 3241 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa 3301 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt 3361 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa 3421 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag 3481 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt 3541 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt 3601 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 3661 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 3721 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 3781 ttcttccatt tcaggtgtcg tgaaccggtg ccaccatggc cagcgacatg tacattgaac 3841 gcgcgggtga cattacgtgg gaaaaagacg ctgaggtcac gggcaatagt cctcggcttg 3901 acgtggccct cgacgagtct ggtgatttct cattggtcga ggaggacggc ccgccaatgc 3961 gggagggcgg gggagggtct ggaggtggag ggtccggggc attgtgggat gttcccgccc 4021 ccaaggaggt taaaaaaggc gaaacgaccg atggcgtata tagggtaatg acacgacgcc 4081 ttctgggtag cactcaagtg ggggttggag tcatgcaaga gggagtcttt cataccatgt 4141 ggcatgttac gaaaggcgct gcactccgat ctggagaagg acggctggac ccctattggg 4201 gtgacgtgaa gcaagaccta gtctcctact gtggaccttg gaagctggat gcggcctggg 4261 acggactcag tgaagtacaa ctccttgctg taccgccagg tgagagagca agaaacattc 4321 aaacgctccc cggtatcttc aagacaaagg acggggatat tggtgccgta gccttggatt 4381 accccgcagg tacaagcggt tccccgatcc tcgataaatg tggtagggta atcggtcttt 4441 atggaaacgg ggtagtgata aagaacggca gctatgttag cgctataaca caaggtaaaa 4501 gggaagagga gacacccgtt gagtgttttg agactagtga ttacaaagat gacgatgaca 4561 aagactacaa ggatgatgat gataagggat ccgaatccgg cagtggagcc acgaacttct 4621 ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcctatgtct gaactcatca 4681 aggagaacat gcacatgaaa ctctacatgg aggggaccgt agacaatcat cacttcaagt 4741 gtacaagtga aggggagggg aagccttacg aagggactca aactatgaga attaaggttg 4801 tcgagggcgg cccccttccc tttgcgtttg acatcctcgc caccagtttc ttgtatggtt 4861 ccaagacgtt tataaatcat acacagggaa tacctgactt ctttaaacaa agttttccag 4921 agggtttcac atgggaaagg gttaccactt atgaggatgg cggagtactc accgctaccc 4981 aagatacatc tctgcaagat ggttgcctga tttacaatgt aaaaatccga ggagttaact 5041 ttacatccaa tggtcctgtt atgcagaaaa agacattggg gtgggaagca tttacagaaa 5101 cgctctaccc cgccgacggg ggcttggagg gccgcaatga catggctctc aaattggtag 5161 gtggtagtca tcttatcgcg aacgcgaaaa ctacctaccg atccaagaag cccgctaaaa 5221 atctcaaaat gccggtctat tacgtggact acagactgga acgcaaggaa gctaacaacg 5281 agacatacgt tgagcaacat gaagtggccg tggccagata ttgcgatctg ccgagtaagc 5341 tcgggcacaa attgaactaa acgcgttaag tcgacaatca acctctggat tacaaaattt 5401 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 5461 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 5521 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 5581 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 5641 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 5701 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 5761 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 5821 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 5881 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 5941 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt 6001 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6061 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6121 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6181 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6241 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga 6301 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 6361 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 6421 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 6481 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 6541 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 6601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 6661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 6721 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 6781 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 6841 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 6901 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 6961 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 7021 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 7081 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 7141 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 7201 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 7261 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 7321 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 7381 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag 7441 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac 7501 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag 7561 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac 7621 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac 7681 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc 7741 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc 7801 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc 7861 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 7921 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat 7981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 8041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg 8101 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 8161 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 8221 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 8281 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 8341 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 8401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 8461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 8521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 8581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 8881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 8941 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 9001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 9061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 9121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 9181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 9241 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 9301 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 9361 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 9421 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 9481 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 9541 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9601 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9661 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9721 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9781 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9841 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9901 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9961 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 10021 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 10081 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 10141 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 10201 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 10261 tttccccgaa aagtgccacc tgac //