LOCUS       Exported               12625 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter and loxP flanked stop cassette, for cloning into AsiSI or 
            Mlui.
ACCESSION   .
VERSION     .
KEYWORDS    pR26 CAG AsiSI/MluI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12625)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 12625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12625
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    26..1108
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    1131..1234
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     intron          1478..1543
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1590..1724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1856..1903
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     enhancer        1918..2297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2299..2574
                     /label=chicken beta-actin promoter
     intron          2575..3591
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3515..3537
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    3638..3671
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3768..4367
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3768..3787)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4264..4284
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4597..4731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4733..4766
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(4857..4873)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4857..4873)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4887..4904)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4893..5117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(5161..5178)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5183..5317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5390..9730
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(9780..9800)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9780..9798)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9819..9835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9819..9835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9832..9854)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9843..9859
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9867..9897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9912..9933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10050..10067)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10221..10809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10301..10320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10980..11840)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11603..11622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11841..11945)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11972..12427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12059..12078)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12269..12290
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12553..12575
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12567..12584
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12568..12584
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12594..12613
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12594..12612
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccaccgcatt accctgttat ccctagcggc aggccctccg agcgtggtgg agccgttctg
       61 tgagacagcc gggtacgagt cgtgacgctg gaaggggcaa gcgggtggtg ggcaggaatg
      121 cggtccgccc tgcagcaacc ggagggggag ggagaaggga gcggaaaagt ctccaccgga
      181 cgcggccatg gctcgggggg gggggggcag cggaggagcg cttccggccg acgtctcgtc
      241 gctgattggc ttcttttcct cccgccgtgt gtgaaaacac aaatggcgtg ttttggttgg
      301 cgtaaggcgc ctgtcagtta acggcagccg gagtgcgcag ccgccggcag cctcgctctg
      361 cccactgggt ggggcgggag gtaggtgggg tgaggcgagc tggacgtgcg ggcgcggtcg
      421 gcctctggcg gggcgggggr ggggagggag ggtcagcgaa agtagctcgc gcgcgagcgg
      481 ccgcccaccc tccccttcct ctgggggagt cgttttaccc gccgccggcc gggcctcgtc
      541 gtctgattgg ctctcggggc ccagaaaact ggcccttgcc attggctcgt gttcgtgcaa
      601 gttgagtcca tccgccggcc agcgggggcg gcgaggaggc gctcccaggt tccggccctc
      661 ccctcggccc cgcgccgcag agtctggccg cgcgcccctg cgcaacgtgg caggaagcgc
      721 gcgctggggg cggggacggg cagtagggct gagcggctgc ggggcgggtg caagcacgtt
      781 tccgacttga gttgcctcaa gaggggcgtg ctgagccaga cctccatcgc gcactccggg
      841 gagtggaggg aaggagcgag ggctcagttg ggctgttttg gaggcaggaa gcacttgctc
      901 tcccaaagtc gctctgagtt gttatcagta agggagctgc agtggagtag gcggggagaa
      961 ggccgcaccc ttctccggag gggggagggg agtgttgcaa tacctttctg ggagttctct
     1021 gctgcctcct ggcttctgag gaccgccctg ggcctgggag aatcccttcc ccctcttccc
     1081 tcgtgatctg caactccagt ctttctaggc gcgcccgggc tgcagatctg tagggcgcag
     1141 tagtccaggg tttccttgat gatgtcatac ttatcctgtc cctttttttt ccacagctcg
     1201 cggttgagga caaactcttc gcggtctttc cagtggggat cgacggtatc gataagctgg
     1261 ccgctctagt ggccgtacgg gcccacctgc cgggccactt aattaaattt aaatcacaat
     1321 tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
     1381 cgggcagggg ggatctgcat ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     1441 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     1501 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     1561 tgatgaatgg gagcagtggt ggaatgccag atccagacat gataagatac attgatgagt
     1621 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1681 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1741 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     1801 tctacaaatg tggtatggct gattatgatc tgcggccgtg ctagcgctta agcttgaagt
     1861 tcctattccg aagttcctat tctctagaaa gtataggaac ttcggcgcgc cggattcgac
     1921 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     1981 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2041 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2101 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2161 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2221 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2281 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2341 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2401 gggcgggggg ggggggggsg cgcgccaggc ggggsggggs ggggsgrggg gsggggsggg
     2461 gsgrggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2521 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2581 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     2641 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     2701 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     2761 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     2821 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     2881 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     2941 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     3001 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     3061 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     3121 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     3181 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     3241 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     3301 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     3361 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     3421 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     3481 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     3541 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gatccttaat
     3601 taaccgtctt aagcctgtaa caaccggtac agttcgaata acttcgtata gcatacatta
     3661 tacgaagtta taagcttgca tgcctgcagg tcggccgcca cgaccggccg gtgccgccac
     3721 catcccctga cccacgcccc tgacccctca caaggagacg accttccatg accgagtaca
     3781 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     3841 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     3901 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     3961 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     4021 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     4081 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     4141 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     4201 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     4261 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     4321 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     4381 acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga agccacccgg
     4441 ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagag gatcataatc
     4501 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4561 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4621 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4681 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccataacttc
     4741 gtatagcata cattatacga agttataccg ggccaccata gtcgcgagta gcttgggcca
     4801 gctaggcctt gaccaaagtt cctctggaat tgcgatcgca cgcgtaaatg attgcagatc
     4861 cactagttct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4921 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4981 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5041 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5101 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga gcctctagaa
     5161 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
     5221 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5281 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5341 tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccc tagaagatgg
     5401 gcgggagtct tctgggcagg cttaaaggct aacctggtgt gtgggcgttg tcctgcaggg
     5461 gaattgaaca ggtgtaaaat tggagggaca agacttccca cagattttcg gttttgtcgg
     5521 gaagtttttt aataggggca aataaggaaa atgggaggat aggtagtcat ctggggtttt
     5581 atgcagcaaa actacaggtt attattgctt gtgatccgcc tcggagtatt ttccatcgag
     5641 gtagattaaa gacatgctca cccgagtttt atactctcct gcttgagatc cttactacag
     5701 tatgaaatta cagtgtcgcg agttagacta tgtaagcaga attttaatca tttttaaaga
     5761 gcccagtact tcatatccat ttctcccgct ccttctgcag ccttatcaaa aggtatttta
     5821 gaacactcat tttagcccca ttttcattta ttatactggc ttatccaacc cctagacaga
     5881 gcattggcat tttccctttc ctgatcttag aagtctgatg actcatgaaa ccagacagat
     5941 tagttacata caccacaaat cgaggctgta gctggggcct caacactgca gttcttttat
     6001 aactccttag tacacttttt gttgatcctt tgccttgatc cttaattttc agtgtctatc
     6061 acctctcccg tcaggtggtg ttccacattt gggcctattc tcagtccagg gagttttaca
     6121 acaatagatg tattgagaat ccaacctaaa gcttaacttt ccactcccat gaatgcctct
     6181 ctcctttttc tccatttata aactgagcta ttaaccatta atggtttcca ggtggatgtc
     6241 tcctccccca atattacctg atgtatctta catattgcca ggctgatatt ttaagacatt
     6301 aaaaggtata tttcattatt gagccacatg gtattgatta ctgcttacta aaattttgtc
     6361 attgtacaca tctgtaaaag gtggttcctt ttggaatgca aagttcaggt gtttgttgtc
     6421 tttcctgacc taaggtcttg tgagcttgta ttttttctat ttaagcagtg ctttctcttg
     6481 gactggcttg actcatggca ttctacacgt tattgctggt ctaaatgtga ttttgccaag
     6541 cttcttcagg acctataatt ttgcttgact tgtagccaaa cacaagtaaa atgattaagc
     6601 aacaaatgta tttgtgaagc ttggttttta ggttgttgtg ttgtgtgtgc ttgtgctcta
     6661 taataatact atccaggggc tggagaggtg gctcggagtt caagagcaca gactgctctt
     6721 ccagaagtcc tgagttcaat tcccagcaac cacatggtgg ctcacaacca tctgtaatgg
     6781 gatctgatgc cctcttctgg tgtgtctgaa gaccacaagt gtattcacat taaataaata
     6841 aatcctcctt cttcttcttt tttttttttt taaagagaat actgtctcca gtagaattta
     6901 ctgaagtaat gaaatacttt gtgtttgttc caatatggta gccaataatc aaattactct
     6961 ttaagcactg gaaatgttac caaggaacta atttttattt gaagtgtaac tgtggacaga
     7021 ggagccataa ctgcagactt gtgggataca gaagaccaat gcagacttta atgtcttttc
     7081 tcttacacta agcaataaag aaataaaaat tgaacttcta gtatcctatt tgtttaaact
     7141 gctagcttta cttaactttt gtgcttcatc tatacaaagc tgaaagctaa gtctgcagcc
     7201 attactaaac atgaaagcaa gtaatgataa ttttggattt caaaaatgta gggccagagt
     7261 ttagccagcc agtggtggtg cttgccttta tgcctttaat cccagcactc tggaggcaga
     7321 gacaggcaga tctctgagtt tgagcccagc ctggtctaca catcaagttc tatctaggat
     7381 agccaggaat acacacagaa accctgttgg ggaggggggc tctgagattt cataaaatta
     7441 taattgaagc attccctaat gagccactat ggatgtggct aaatccgtct acctttctga
     7501 tgagatttgg gtattatttt ttctgtctct gctgttggtt gggtcttttg acactgtggg
     7561 ctttctttaa agcctccttc ctgccatgtg gtctcttgtt tgctactaac ttcccatggc
     7621 ttaaatggca tggctttttg ccttctaagg gcagctgctg agatttgcag cctgatttcc
     7681 agggtggggt tgggaaatct ttcaaacact aaaattgtcc tttaattttt tttttaaaaa
     7741 atgggttata taataaacct cataaaatag ttatgaggag tgaggtggac taatattaaa
     7801 tgagtccctc ccctataaaa gagctattaa ggctttttgt cttatactta actttttttt
     7861 taaatgtggt atctttagaa ccaagggtct tagagtttta gtatacagaa actgttgcat
     7921 cgcttaatca gattttctag tttcaaatcc agagaatcca aattcttcac agccaaagtc
     7981 aaattaagaa tttctgactt ttaatgttaa tttgcttact gtgaatataa aaatgatagc
     8041 ttttcctgag gcagggtctc actatgtatc tctgcctgat ctgcaacaag atatgtagac
     8101 taaagttctg cctgcttttg tctcctgaat actaaggtta aaatgtagta atacttttgg
     8161 aacttgcagg tcagattctt ttatagggga cacactaagg gagcttgggt gatagttggt
     8221 aaaatgtgtt tcaagtgatg aaaacttgaa ttattatcac cgcaacctac tttttaaaaa
     8281 aaaaagccag gcctgttaga gcatgcttaa gggatcccta ggacttgctg agcacacaag
     8341 agtagttact tggcaggctc ctggtgagag catatttcaa aaaacaaggc agacaaccaa
     8401 gaaactacag ttaaggttac ctgtctttaa accatctgca tatacacagg gatattaaaa
     8461 tattccaaat aatatttcat tcaagttttc ccccatcaaa ttgggacatg gatttctccg
     8521 gtgaataggc agagttggaa actaaacaaa tgttggtttt gtgatttgtg aaattgtttt
     8581 caagtgatag ttaaagccca tgagatacag aacaaagctg ctatttcgag gtctcttggt
     8641 ttatactcag aagcacttct ttgggtttcc ctgcactatc ctgatcatgt gctaggccta
     8701 ccttaggctg attgttgttc aaataaactt aagtttcctg tcaggtgatg tcatatgatt
     8761 tcatatatca aggcaaaaca tgttatatat gttaaacatt tgtacttaat gtgaaagtta
     8821 ggtctttgtg ggtttgattt ttaattttca aaacctgagc taaataagtc atttttacat
     8881 gtcttacatt tggtggaatt gtataattgt ggtttgcagg caagactctc tgacctagta
     8941 accctaccta tagagcactt tgctgggtca caagtctagg agtcaagcat ttcaccttga
     9001 agttgagacg ttttgttagt gtatactagt ttatatgttg gaggacatgt ttatccagaa
     9061 gatattcagg actatttttg actgggctaa ggaattgatt ctgattagca ctgttagtga
     9121 gcattgagtg gcctttaggc ttgaattgga gtcacttgta tatctcaaat aatgctggcc
     9181 ttttttaaaa agcccttgtt ctttatcacc ctgttttcta cataattttt gttcaaagaa
     9241 atacttgttt ggatctcctt ttgacaacaa tagcatgttt tcaagccata ttttttttcc
     9301 tttttttttt tttttttggt ttttcgagac agggtttctc tgtatagccc tggctgtcct
     9361 ggaactcact ttgtagacca ggctggcctc gaactcagaa atccgcctgc ctctgcctcc
     9421 tgagtgccgg gattaaaggc gtgcaccacc acgcctggct aagttggata ttttgttata
     9481 taactataac caatactaac tccactgggt ggatttttaa ttcagtcagt agtcttaagt
     9541 ggtctttatt ggcccttcat taaaatctac tgttcactct aacagaggct gttggtacta
     9601 gtggcactta agcaacttcc tacggatata ctagcagatt aagggtcagg gatagaaact
     9661 agtctagcgt tttgtatacc taccagcttt atactacctt gttctgatag aaatatttca
     9721 ggacatctag cttatcgata ccgtcgacgg tatcgataag cttgatccag cttttgttcc
     9781 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     9841 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9901 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10021 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10201 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10981 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11041 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11101 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11161 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11221 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11281 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11341 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11401 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11461 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11521 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11581 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11641 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11701 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11761 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11821 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11881 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11941 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
    12001 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    12061 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    12121 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    12181 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    12241 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    12301 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    12361 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    12421 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    12481 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    12541 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
    12601 actcactata gggcgaattg gagct
//
