LOCUS Exported 10790 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of BRAF T241P. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-T241P SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 19..1197 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 250..1188 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1145..1165 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1217..1241 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1242..1251 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3546..3570) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3578..3596 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3621..3640) /label=T7 /note="T7 promoter, forward primer" promoter complement(3622..3640) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(3646..3666) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 3720..3761 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3782..4349 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3799..4351 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3943..3960) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4170..4189 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4363..4962 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4363..4382) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4859..4879 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 4976..5564 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5029..5049) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5447..5458) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5639..5872 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(5991..6009) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6077..6181 /gene="bla" /label=AmpR promoter CDS 6182..7042 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6400..6419) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7213..7801 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7702..7721 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7955..7972 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8064..8083 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8212..8233 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8248..8278 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8286..8302 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8291..8313 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8310..8326 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8310..8326 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8376..8395 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8587..9220 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9267..9392 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9889..10122 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10307..10351 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10644..10761 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 61 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 121 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 181 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 241 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 301 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt 361 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 421 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 481 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 541 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 601 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 661 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 721 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 781 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 841 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 901 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 961 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 1021 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 1081 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 1141 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc 1201 ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc accatggcgg cgctgagcgg 1261 tggcggtggt ggcggcgcgg agccgggcca ggctctgttc aacggggaca tggagcccga 1321 ggccggcgcc ggcgccggcg ccgcggcctc ttcggctgcg gaccctgcca ttccggagga 1381 ggtgtggaat atcaaacaaa tgattaagtt gacacaggaa catatagagg ccctattgga 1441 caaatttggt ggggagcata atccaccatc aatatatctg gaggcctatg aagaatacac 1501 cagcaagcta gatgcactcc aacaaagaga acaacagtta ttggaatctc tggggaacgg 1561 aactgatttt tctgtttcta gctctgcatc aatggatacc gttacatctt cttcctcttc 1621 tagcctttca gtgctacctt catctctttc agtttttcaa aatcccacag atgtggcacg 1681 gagcaacccc aagtcaccac aaaaacctat cgttagagtc ttcctgccca acaaacagag 1741 gacagtggta cctgcaaggt gtggagttac agtccgagac agtctaaaga aagcactgat 1801 gatgagaggt ctaatcccag agtgctgtgc tgtttacaga attcaggatg gagagaagaa 1861 accaattggt tgggacactg atatttcctg gcttactgga gaagaattgc atgtggaagt 1921 gttggagaat gttccactta caacacacaa ctttgtacga aaaccgtttt tcaccttagc 1981 attttgtgac ttttgtcgaa agctgctttt ccagggtttc cgctgtcaaa catgtggtta 2041 taaatttcac cagcgttgta gtacagaagt tccactgatg tgtgttaatt atgaccaact 2101 tgatttgctg tttgtctcca agttctttga acaccaccca ataccacagg aagaggcgtc 2161 cttagcagag actgccctaa catctggatc atccccttcc gcacccgcct cggactctat 2221 tgggccccaa attctcacca gtccgtctcc ttcaaaatcc attccaattc cacagccctt 2281 ccgaccagca gatgaagatc atcgaaatca atttgggcaa cgagaccgat cctcatcagc 2341 tcccaatgtg catataaaca caatagaacc tgtcaatatt gatgacttga ttagagacca 2401 aggatttcgt ggtgatggag gatcaaccac aggtttgtct gctacccccc ctgcctcatt 2461 acctggctca ctaactaacg tgaaagcctt acagaaatct ccaggacctc agcgagaaag 2521 gaagtcatct tcatcctcag aagacaggaa tcgaatgaaa acacttggta gacgggactc 2581 gagtgatgat tgggagattc ctgatgggca gattacagtg ggacaaagaa ttggatctgg 2641 atcatttgga acagtctaca agggaaagtg gcatggtgat gtggcagtga aaatgttgaa 2701 tgtgacagca cctacacctc agcagttaca agccttcaaa aatgaagtag gagtactcag 2761 gaaaacacga catgtgaata tcctactctt catgggctat tccacaaagc cacaactggc 2821 tattgttacc cagtggtgtg agggctccag cttgtatcac catctccata tcattgagac 2881 caaatttgag atgatcaaac ttatagatat tgcacgacag actgcacagg gcatggatta 2941 cttacacgcc aagtcaatca tccacagaga cctcaagagt aataatatat ttcttcatga 3001 agacctcaca gtaaaaatag gtgattttgg tctagctaca gtgaaatctc gatggagtgg 3061 gtcccatcag tttgaacagt tgtctggatc cattttgtgg atggcaccag aagtcatcag 3121 aatgcaagat aaaaatccat acagctttca gtcagatgta tatgcatttg gaattgttct 3181 gtatgaattg atgactggac agttacctta ttcaaacatc aacaacaggg accagataat 3241 ttttatggtg ggacgaggat acctgtctcc agatctcagt aaggtacgga gtaactgtcc 3301 aaaagccatg aagagattaa tggcagagtg cctcaaaaag aaaagagatg agagaccact 3361 ctttccccaa attctcgcct ctattgagct gctggcccgc tcattgccaa aaattcaccg 3421 cagtgcatca gaaccctcct tgaatcgggc tggtttccaa acagaggatt ttagtctata 3481 tgcttgtgct tctccaaaaa cacccatcca ggcaggggga tatggtgcgt ttcctgtcca 3541 ctagaaccca gctttcttgt acaaagtggt gatatccaat taaccctcac taaaggttta 3601 agtgagttat agaagtagta ccctatagtg agtcgtatta ccacccaact tttctataca 3661 aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag 3721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag 3781 atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt 3841 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac 3901 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc 3961 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa 4021 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg 4081 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg 4141 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc 4201 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat 4261 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg 4321 tggttttcct ttgaaaaaca cgatgataat atggccacac atatgaccga gtacaagccc 4381 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4441 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4501 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4561 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4621 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4681 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 4741 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 4801 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 4861 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 4921 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aaatcgatag atcctaatca 4981 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 5041 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 5101 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 5161 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 5221 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 5281 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 5341 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg 5401 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 5461 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 5521 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga 5581 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 5641 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc 5701 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5761 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5821 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5881 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 5941 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat 6001 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac 6061 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 6121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 6181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 6241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 6301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 6361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 6421 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 6481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 6541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 6601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 6661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 6721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 6781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 6841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 6901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 6961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 7021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 7081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 7141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 7201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 7261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 7321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 7381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 7441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 7501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 7561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 7621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 7681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 7741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 7801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 7861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 7921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 7981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa 8041 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 8101 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc 8161 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 8221 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 8281 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt 8341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 8401 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 8461 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga 8521 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt 8581 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct 8641 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat 8701 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag 8761 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg 8821 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg 8881 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac 8941 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc 9001 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag 9061 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc 9121 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga 9181 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact 9241 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc 9301 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag 9361 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc 9421 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag 9481 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag 9541 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac 9601 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa 9661 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg 9721 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg 9781 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac 9841 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt 9901 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt 9961 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat 10021 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag 10081 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc 10141 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct 10201 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 10261 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 10321 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 10381 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 10441 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 10501 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 10561 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca 10621 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca 10681 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa 10741 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc //