LOCUS Exported 10790 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral expression of BRAF E648Q. ACCESSION . VERSION . KEYWORDS pHAGE-BRAF-E648Q SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10790) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 10790) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10790 /organism="synthetic DNA construct" /mol_type="other DNA" intron 221..1159 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1116..1136 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1188..1212 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 1213..1222 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3517..3541) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 3549..3567 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3592..3611) /label=T7 /note="T7 promoter, forward primer" promoter complement(3593..3611) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(3617..3637) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 3691..3732 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3753..4320 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 3770..4322 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3914..3931) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4141..4160 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4334..4933 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4334..4353) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4830..4850 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 4947..5535 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5000..5020) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5418..5429) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5610..5843 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(5962..5980) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6048..6152 /gene="bla" /label=AmpR promoter CDS 6153..7013 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6371..6390) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7184..7772 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7673..7692 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7926..7943 /label=L4440 /note="L4440 vector, forward primer" primer_bind 8035..8054 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 8183..8204 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8219..8249 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8257..8273 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8262..8284 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 8281..8297 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 8281..8297 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 8347..8366 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 8558..9191 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 9238..9363 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9860..10093 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10278..10322 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 10615..10732 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 10780..1168 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" ORIGIN 1 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg 61 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta 121 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt 181 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc 241 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg 301 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag 361 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg 421 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc 481 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct 541 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga 601 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc 661 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc 721 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc 781 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc 841 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt 901 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag 961 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca 1021 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga 1081 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag 1141 tttttttctt ccatttcagg tgtcgtgaag cggccctgca gatatcaaca agtttgtaca 1201 aaaaagcagg caccatggcg gcgctgagcg gtggcggtgg tggcggcgcg gagccgggcc 1261 aggctctgtt caacggggac atggagcccg aggccggcgc cggcgccggc gccgcggcct 1321 cttcggctgc ggaccctgcc attccggagg aggtgtggaa tatcaaacaa atgattaagt 1381 tgacacagga acatatagag gccctattgg acaaatttgg tggggagcat aatccaccat 1441 caatatatct ggaggcctat gaagaataca ccagcaagct agatgcactc caacaaagag 1501 aacaacagtt attggaatct ctggggaacg gaactgattt ttctgtttct agctctgcat 1561 caatggatac cgttacatct tcttcctctt ctagcctttc agtgctacct tcatctcttt 1621 cagtttttca aaatcccaca gatgtggcac ggagcaaccc caagtcacca caaaaaccta 1681 tcgttagagt cttcctgccc aacaaacaga ggacagtggt acctgcaagg tgtggagtta 1741 cagtccgaga cagtctaaag aaagcactga tgatgagagg tctaatccca gagtgctgtg 1801 ctgtttacag aattcaggat ggagagaaga aaccaattgg ttgggacact gatatttcct 1861 ggcttactgg agaagaattg catgtggaag tgttggagaa tgttccactt acaacacaca 1921 actttgtacg aaaaacgttt ttcaccttag cattttgtga cttttgtcga aagctgcttt 1981 tccagggttt ccgctgtcaa acatgtggtt ataaatttca ccagcgttgt agtacagaag 2041 ttccactgat gtgtgttaat tatgaccaac ttgatttgct gtttgtctcc aagttctttg 2101 aacaccaccc aataccacag gaagaggcgt ccttagcaga gactgcccta acatctggat 2161 catccccttc cgcacccgcc tcggactcta ttgggcccca aattctcacc agtccgtctc 2221 cttcaaaatc cattccaatt ccacagccct tccgaccagc agatgaagat catcgaaatc 2281 aatttgggca acgagaccga tcctcatcag ctcccaatgt gcatataaac acaatagaac 2341 ctgtcaatat tgatgacttg attagagacc aaggatttcg tggtgatgga ggatcaacca 2401 caggtttgtc tgctaccccc cctgcctcat tacctggctc actaactaac gtgaaagcct 2461 tacagaaatc tccaggacct cagcgagaaa ggaagtcatc ttcatcctca gaagacagga 2521 atcgaatgaa aacacttggt agacgggact cgagtgatga ttgggagatt cctgatgggc 2581 agattacagt gggacaaaga attggatctg gatcatttgg aacagtctac aagggaaagt 2641 ggcatggtga tgtggcagtg aaaatgttga atgtgacagc acctacacct cagcagttac 2701 aagccttcaa aaatgaagta ggagtactca ggaaaacacg acatgtgaat atcctactct 2761 tcatgggcta ttccacaaag ccacaactgg ctattgttac ccagtggtgt gagggctcca 2821 gcttgtatca ccatctccat atcattgaga ccaaatttga gatgatcaaa cttatagata 2881 ttgcacgaca gactgcacag ggcatggatt acttacacgc caagtcaatc atccacagag 2941 acctcaagag taataatata tttcttcatg aagacctcac agtaaaaata ggtgattttg 3001 gtctagctac agtgaaatct cgatggagtg ggtcccatca gtttgaacag ttgtctggat 3061 ccattttgtg gatggcacca gaagtcatca gaatgcaaga taaaaatcca tacagctttc 3121 agtcagatgt atatgcattt ggaattgttc tgtatcaatt gatgactgga cagttacctt 3181 attcaaacat caacaacagg gaccagataa tttttatggt gggacgagga tacctgtctc 3241 cagatctcag taaggtacgg agtaactgtc caaaagccat gaagagatta atggcagagt 3301 gcctcaaaaa gaaaagagat gagagaccac tctttcccca aattctcgcc tctattgagc 3361 tgctggcccg ctcattgcca aaaattcacc gcagtgcatc agaaccctcc ttgaatcggg 3421 ctggtttcca aacagaggat tttagtctat atgcttgtgc ttctccaaaa acacccatcc 3481 aggcaggggg atatggtgcg tttcctgtcc actagaaccc agctttcttg tacaaagtgg 3541 tgatatccaa ttaaccctca ctaaaggttg tatgtgagtt tagattaatg accctatagt 3601 gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca cagtggcggc 3661 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt 3721 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc 3781 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 3841 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 3901 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 3961 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 4021 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 4081 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 4141 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 4201 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 4261 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 4321 tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 4381 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 4441 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 4501 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 4561 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 4621 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 4681 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 4741 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 4801 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 4861 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 4921 gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat 4981 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 5041 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 5101 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 5161 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 5221 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 5281 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 5341 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 5401 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 5461 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 5521 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct 5581 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 5641 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 5701 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 5761 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 5821 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 5881 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag 5941 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 6001 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 6061 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 6121 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 6181 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 6241 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 6301 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 6361 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg 6421 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 6481 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 6541 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 6601 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 6661 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 6721 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 6781 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 6841 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 6901 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 6961 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 7021 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 7081 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 7141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 7201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 7261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 7321 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 7381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 7441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 7501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 7561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 7621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 7681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 7741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 7801 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 7861 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 7921 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 7981 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta 8041 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 8101 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag 8161 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 8221 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 8281 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt 8341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa 8401 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt 8461 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa 8521 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa 8581 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag 8641 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag 8701 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg 8761 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg 8821 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag 8881 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt 8941 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt 9001 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 9061 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 9121 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 9181 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc 9241 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg 9301 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc 9361 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg 9421 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc 9481 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta 9541 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc 9601 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata 9661 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca 9721 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag 9781 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag 9841 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca 9901 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag 9961 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca 10021 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg 10081 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc 10141 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga 10201 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat 10261 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt 10321 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag 10381 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta 10441 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca 10501 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag 10561 tgaacggatc tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa 10621 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 10681 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta 10741 ttacagggac agcagagatc cagtttggac tagtcgtgag gctccggtgc //