LOCUS       Exported               10957 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of FGFR3 V788M.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-FGFR3-V788M
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    100..217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        265..1443
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          496..1434
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1391..1411
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1463..1487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3936..3960)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4013..4054
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4075..4642
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4092..4644
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4236..4253)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4463..4482
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4656..5375
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4656..5372
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4701..4722)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4962..4981)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5309..5330
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5389..5977
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5442..5462)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5860..5871)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6052..6285
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6404..6422)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6490..6594
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6595..7455
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6813..6832)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7626..8214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8115..8134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8368..8385
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8477..8496
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    8625..8646
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8661..8691
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8699..8715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8704..8726
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8723..8739
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8723..8739
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8789..8808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9000..9633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9680..9805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10302..10535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10720..10764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
       61 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
      121 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
      181 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
      241 agatccagtt tggactagtc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
      301 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
      361 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      421 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      481 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
      541 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
      601 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
      661 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
      721 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
      781 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
      841 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
      901 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
      961 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     1021 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     1081 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     1141 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     1201 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
     1261 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     1321 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     1381 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     1441 tgaagcggcc ctgcagatat caacaagttt gtacaaaaaa gcagaagggc cgtcaaggcc
     1501 caccatgggc gcccctgcct gcgccctcgc gctctgcgtg gccgtggcca tcgtggccgg
     1561 cgcctcctcg gagtccttgg ggacggagca gcgcgtcgtg gggcgagcgg cagaagtccc
     1621 gggcccagag cccggccagc aggagcagtt ggtcttcggc agcggggatg ctgtggagct
     1681 gagctgtccc ccgcccgggg gtggtcccat ggggcccact gtctgggtca aggatggcac
     1741 agggctggtg ccctcggagc gtgtcctggt ggggccccag cggctgcagg tgctgaatgc
     1801 ctcccacgag gactccgggg cctacagctg ccggcagcgg ctcacgcagc gcgtactgtg
     1861 ccacttcagt gtgcgggtga cagacgctcc atcctcggga gatgacgaag acggggagga
     1921 cgaggctgag gacacaggtg tggacacagg ggccccttac tggacacggc ccgagcggat
     1981 ggacaagaag ctgctggccg tgccggccgc caacaccgtc cgcttccgct gcccagccgc
     2041 tggcaacccc actccctcca tctcctggct gaagaacggc agggagttcc gcggcgagca
     2101 ccgcattgga ggcatcaagc tgcggcatca gcagtggagc ctggtcatgg aaagcgtggt
     2161 gccctcggac cgcggcaact acacctgcgt cgtggagaac aagtttggca gcatccggca
     2221 gacgtacacg ctggacgtgc tggagcgctc cccgcaccgg cccatcctgc aggcggggct
     2281 gccggccaac cagacggcgg tgctgggcag cgacgtggag ttccactgca aggtgtacag
     2341 tgacgcacag ccccacatcc agtggctcaa gcacgtggag gtgaacggca gcaaggtggg
     2401 cccggacggc acaccctacg ttaccgtgct caagacggcg ggcgctaaca ccaccgacaa
     2461 ggagctagag gttctctcct tgcacaacgt cacctttgag gacgccgggg agtacacctg
     2521 cctggcgggc aattctattg ggttttctca tcactctgcg tggctggtgg tgctgccagc
     2581 cgaggaggag ctggtggagg ctgacgaggc gggcagtgtg tatgcaggca tcctcagcta
     2641 cggggtgggc ttcttcctgt tcatcctggt ggtggcggct gtgacgctct gccgcctgcg
     2701 cagccccccc aagaaaggcc tgggctcccc caccgtgcac aagatctccc gcttcccgct
     2761 caagcgacag gtgtccctgg agtccaacgc gtccatgagc tccaacacac cactggtgcg
     2821 catcgcaagg ctgtcctcag gggagggccc cacgctggcc aatgtctccg agctcgagct
     2881 gcctgccgac cccaaatggg agctgtctcg ggcccggctg accctgggca agccccttgg
     2941 ggagggctgc ttcggccagg tggtcatggc ggaggccatc ggcattgaca aggaccgggc
     3001 cgccaagcct gtcaccgtag ccgtgaagat gctgaaagac gatgccactg acaaggacct
     3061 gtcggacctg gtgtctgaga tggagatgat gaagatgatc gggaaacaca aaaacatcat
     3121 caacctgctg ggcgcctgca cgcagggcgg gcccctgtac gtgctggtgg agtacgcggc
     3181 caagggtaac ctgcgggagt ttctgcgggc gcggcggccc ccgggcctgg actactcctt
     3241 cgacacctgc aagccgcccg aggagcagct caccttcaag gacctggtgt cctgtgccta
     3301 ccaggtggcc cggggcatgg agtacttggc ctcccagaag tgcatccaca gggacctggc
     3361 tgcccgcaat gtgctggtga ccgaggacaa cgtgatgaag atcgcagact tcgggctggc
     3421 ccgggacgtg cacaacctcg actactacaa gaagacaacc aacggccggc tgcccgtgaa
     3481 gtggatggcg cctgaggcct tgtttgaccg agtctacact caccagagtg acgtctggtc
     3541 ctttggggtc ctgctctggg agatcttcac gctggggggc tccccgtacc ccggcatccc
     3601 tgtggaggag ctcttcaagc tgctgaagga gggccaccgc atggacaagc ccgccaactg
     3661 cacacacgac ctgtacatga tcatgcggga gtgctggcat gccgcgccct cccagaggcc
     3721 caccttcaag cagctggtgg aggacctgga ccgtgtcctt accgtgacgt ccaccgacga
     3781 gtacctggac ctgtcggcgc ctttcgagca gtactccccg ggtggccagg acacccccag
     3841 ctccagctcc tcaggggacg actccatgtt tgcccacgac ctgctgcccc cggccccacc
     3901 cagcagtggg ggctcgcgga cgtagggcct catgggccca gctttcttgt acaaagtggt
     3961 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     4021 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     4081 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4141 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4201 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4261 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4321 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     4381 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     4441 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     4501 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     4561 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     4621 cctttgaaaa acacgatgat aatatggcca cacatatggt gagcaagggc gaggagctgt
     4681 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4741 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4801 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4861 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4921 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4981 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     5041 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5101 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     5161 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5221 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5281 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5341 ggatcactct cggcatggac gagctgtaca agtaaatcga tagatcctaa tcaacctctg
     5401 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     5461 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     5521 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5581 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5641 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5701 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5761 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     5821 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5881 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5941 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     6001 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6061 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     6121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6301 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     6361 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     6421 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     6481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6601 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6661 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6721 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     6781 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     6841 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     6901 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     6961 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7021 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7081 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7141 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7201 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7261 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7321 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7381 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7441 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8281 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8341 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8401 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
     8461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8521 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
     8581 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8641 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8701 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
     8761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8821 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     8881 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     8941 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     9001 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     9061 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     9121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     9181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     9241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     9301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     9361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     9421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     9661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     9721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     9781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     9841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     9901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     9961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    10021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    10081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    10141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    10201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    10261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    10321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    10381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    10441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    10501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    10561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    10621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    10681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    10741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    10801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    10861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    10921 ccaaccccga ggggacccga caggcccgaa ggaatag
//
