LOCUS Exported 11700 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral expression of PIK3CB D1067Y. ACCESSION . VERSION . KEYWORDS pHAGE-PIK3CB-D1067Y SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11700) AUTHORS Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z, Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott KL, Yi S, Sahni N, Liang H, Mills GB TITLE Systematic Functional Annotation of Somatic Mutations in Cancer. JOURNAL Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 10.1016/j.ccell.2018.01.021. PUBMED 29533785 REFERENCE 2 (bases 1 to 11700) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11700 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 85..202 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 250..1428 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 481..1419 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1376..1396 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1448..1472 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" RBS 1683..1691 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS complement(2990..3001) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind complement(4687..4711) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 4719..4737 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4762..4781) /label=T7 /note="T7 promoter, forward primer" promoter complement(4763..4781) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind complement(4787..4807) /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" CDS 4861..4902 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 4923..5490 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 4940..5492 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5084..5101) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5311..5330 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5504..6103 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5504..5523) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6000..6020 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6117..6705 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6170..6190) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6588..6599) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6780..7013 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(7132..7150) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7218..7322 /gene="bla" /label=AmpR promoter CDS 7323..8183 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7541..7560) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8354..8942 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8843..8862 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9096..9113 /label=L4440 /note="L4440 vector, forward primer" primer_bind 9205..9224 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 9353..9374 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 9389..9419 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9427..9443 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 9432..9454 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 9451..9467 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 9451..9467 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 9517..9536 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 9728..10361 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 10408..10533 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 11030..11263 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 11448..11492 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" ORIGIN 1 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc 61 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc 121 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca 181 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac 241 tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 301 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac 361 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 421 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 481 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg 541 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga 601 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt 661 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt 721 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt 781 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt 841 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg 901 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct 961 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg 1021 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca 1081 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg 1141 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg 1201 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt 1261 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac 1321 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag 1381 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccctgca 1441 gatatcaaca agtttgtaca aaaaagcagg caccatgtgc ttcagtttca taatgcctcc 1501 tgctatggca gacatccttg acatctgggc ggtggattca cagatagcat ctgatggctc 1561 catacctgtg gatttccttt tgcccactgg gatttatatc cagttggagg tacctcggga 1621 agctaccatt tcttatatta agcagatgtt atggaagcaa gttcacaatt acccaatgtt 1681 caacctcctt atggatattg actcctatat gtttgcatgt gtgaatcaga ctgctgtata 1741 tgaggagctt gaagatgaaa cacgaagact ctgtgatgtc agaccttttc ttccagttct 1801 caaattagtg acaagaagtt gtgacccagg ggaaaaatta gactcaaaaa ttggagtcct 1861 tataggaaaa ggtctgcatg aatttgattc cttgaaggat cctgaagtaa atgaatttcg 1921 aagaaaaatg cgcaaattca gcgaggaaaa aatcctgtca cttgtgggat tgtcttggat 1981 ggactggcta aaacaaacat atccaccaga gcatgaacca tccatccctg aaaacttaga 2041 agataaactt tatgggggaa agctcatcgt agctgttcat tttgaaaact gccaggacgt 2101 gtttagcttt caagtgtctc ctaatatgaa tcctatcaaa gtaaatgaat tggcaatcca 2161 aaaacgtttg actattcatg ggaaggaaga tgaagttagc ccctatgatt atgtgttgca 2221 agtcagcggg agagtagaat atgtttttgg tgatcatcca ctaattcagt tccagtatat 2281 ccggaactgt gtgatgaaca gagccctgcc ccattttata cttgtggaat gctgcaagat 2341 caagaaaatg tatgaacaag aaatgattgc catagaggct gccataaatc gaaattcatc 2401 taatcttcct cttccattac caccaaagaa aacacgaatt atttctcatg tttgggaaaa 2461 taacaaccct ttccaaattg tcttggttaa gggaaataaa cttaacacag aggaaactgt 2521 aaaagttcat gtcagggctg gtctttttca tggtactgag ctcctgtgta aaaccatcgt 2581 aagctcagag gtatcaggga aaaatgatca tatttggaat gaaccactgg aatttgatat 2641 taatatttgt gacttaccaa gaatggctcg attatgtttt gctgtttatg cagttttgga 2701 taaagtaaaa acgaagaaat caacgaaaac tattaatccc tctaaatatc agaccatcag 2761 gaaagctgga aaagtgcatt atcctgtagc gtgggtaaat acgatggttt ttgactttaa 2821 aggacaattg agaactggag acataatatt acacagctgg tcttcatttc ctgatgaact 2881 cgaagaaatg ttgaatccaa tgggaactgt tcaaacaaat ccatatactg aaaatgcaac 2941 agctttgcat gttaaatttc cagagaataa aaaacaacct tattattacc ctcccttcga 3001 taagattatt gaaaaggcag ctgagattgc aagcagtgat agtgctaatg tgtcaagtcg 3061 aggtggaaaa aagtttcttc ctgtattgaa agaaatcttg gacagggatc ccttgtctca 3121 actgtgtgaa aatgaaatgg atcttatttg gactttgcga caagactgcc gagagatttt 3181 cccacaatca ctgccaaaat tactgctgtc aatcaagtgg aataaacttg aggatgttgc 3241 tcagcttcag gcgctgcttc agatttggcc taaactgccc ccccgggagg ccctagagct 3301 tctggatttc aactatccag accagtacgt tcgagaatat gctgtaggct gcctgcgaca 3361 gatgagtgat gaagaacttt ctcaatatct tttacaactg gtgcaagtgt taaaatatga 3421 gccttttctt gattgtgccc tctctagatt cctattagaa agagcacttg gtaatcggag 3481 gatagggcag tttctatttt ggcatcttag gtcagaagtg cacattcctg ctgtctcagt 3541 acaatttggt gtcatccttg aagcatactg ccggggaagt gtggggcaca tgaaagtgct 3601 ttctaagcag gttgaagcac tcaataagtt aaaaacttta aatagtttaa tcaaactgaa 3661 tgccgtgaag ttaaacagag ccaaagggaa ggaggccatg catacctgtt taaaacagag 3721 tgcttaccgg gaagccctct ctgacctgca gtcacccctg aacccatgtg ttatcctctc 3781 agaactctat gttgaaaagt gcaaatacat ggattccaaa atgaagcctt tgtggctggt 3841 atacaataac aaggtatttg gtgaggattc agttggagtg atttttaaaa atggtgatga 3901 tttacgacag gatatgttga cactccaaat gttgcgcttg atggatttac tctggaaaga 3961 agctggtttg gatcttcgga tgttgcctta tggctgttta gcaacaggag atcgctctgg 4021 cctcattgaa gttgtgagca cctctgaaac aattgctgac attcagctga acagtagcaa 4081 tgtggctgct gcagcagcct tcaacaaaga tgcccttctg aactggctta aagaatacaa 4141 ctctggggat gacctggacc gagccattga ggaatttaca ctgtcctgtg ctggctactg 4201 tgtagcttct tatgtccttg ggattggtga cagacatagt gacaacatca tggtcaaaaa 4261 aactggccag ctcttccaca ttgactttgg acatattctt ggaaatttca aatctaagtt 4321 tggcattaaa agggagcgag tgccttttat tcttacctat gatttcatcc atgtcattca 4381 acaaggaaaa acaggaaata cagaaaagtt tggccggttc cgccagtgtt gtgaggatgc 4441 atatctgatt ttacgacggc atgggaatct cttcatcact ctctttgcgc tgatgttgac 4501 tgcagggctt cctgaactca catcagtcaa agatatacag tatcttaagg actctcttgc 4561 attagggaag agtgaagaag aagcactcaa acagtttaag caaaaatttg atgaggcgct 4621 cagggaaagc tggactacta aagtgaactg gatggcccac acagttcgga aatactacag 4681 atcttaaccc aactttcttg tacaaagtgg tgatatccaa ttaaccctca ctaaagggaa 4741 gatattgaaa gaatttgatg tccctatagt gagtcgtatt accacccaac ttttctatac 4801 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa 4861 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga 4921 gatccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg 4981 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa 5041 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg 5101 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca 5161 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc 5221 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt 5281 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg 5341 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca 5401 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac 5461 gtggttttcc tttgaaaaac acgatgataa tatggccaca catatgaccg agtacaagcc 5521 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc 5581 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt 5641 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt 5701 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc 5761 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca 5821 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc 5881 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc 5941 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg 6001 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga 6061 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc taaatcgata gatcctaatc 6121 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 6181 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 6241 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 6301 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 6361 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 6421 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 6481 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 6541 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 6601 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 6661 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag 6721 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 6781 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 6841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 6901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 6961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 7021 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 7081 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga 7141 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 7201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 7261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 7321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 7381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 7441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 7501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 7561 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 7621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 7681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 7741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 7801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 7861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 7921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 7981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 8041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 8101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 8161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 8221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 8281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 8341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 8401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 8461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 8521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 8581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 8641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 8701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 8761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 8821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 8881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 8941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 9001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 9061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 9121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca 9181 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 9241 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 9301 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 9361 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 9421 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt 9481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 9541 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 9601 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg 9661 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact 9721 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc 9781 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga 9841 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa 9901 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat 9961 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg 10021 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga 10081 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc 10141 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca 10201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 10261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 10321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac 10381 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg 10441 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga 10501 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg 10561 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata 10621 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca 10681 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa 10741 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata 10801 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc 10861 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt 10921 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 10981 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 11041 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 11101 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 11161 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 11221 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 11281 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 11341 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 11401 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 11461 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 11521 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 11581 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 11641 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag //