LOCUS       Exported                9679 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentiviral expression of TAOK1__PIPOX fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-TAOK1__PIPOX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    261..378
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        426..1604
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          657..1595
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1552..1572
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1624..1648
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1852..1876)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2942..2962)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3016..3057
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3078..3645
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3095..3647
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3239..3256)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3466..3485
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3659..4258
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3659..3678)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4155..4175
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4272..4860
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4325..4345)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4743..4754)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4935..5168
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5287..5305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5373..5477
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5478..6338
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5696..5715)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6509..7097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6998..7017
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7251..7268
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7360..7379
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    7508..7529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7544..7574
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7582..7598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7587..7609
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7606..7622
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7606..7622
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7672..7691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7883..8516
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8563..8688
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9185..9418
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9603..9647
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
       61 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
      121 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
      181 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc gaattcacaa
      241 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
      301 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
      361 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggactagt
      421 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
      481 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      541 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      601 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      661 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      721 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      781 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      841 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      901 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
      961 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     1021 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     1081 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     1141 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     1201 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     1261 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     1321 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     1381 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1441 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1501 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1561 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagcggc cctgcagata
     1621 tcaacaagtt tgtacaaaaa agttggcatg ccatcaacta acagagcagg cagcctgaag
     1681 gaccctgaaa ttgcagagct cttcttcaaa gaagatccag agaagctctt cacagatctc
     1741 agagaaattg gccatggaag ctttggagca gtgtattttg cacgagatgt gcgtaccaat
     1801 gaagtggtgg ccatcaagaa aatgtcttat agtggaaagc agtctactga gtcccagctt
     1861 tcttgtacaa agtggttctt ctttctacca cactcccgag gaagctccca tggacaaagc
     1921 cggataatcc gaaaggcgta cctggaagac ttttacaccc ggatgatgca tgagtgctat
     1981 cagatatggg cccagctgga gcacgaggct ggaacccaat tgcacaggca gactggatta
     2041 ctgctgctgg gaatgaaaga gaatcaagaa ttaaagacaa tccaggccaa tctgtcgagg
     2101 cagagggtag aacaccagtg tctttcatct gaggaactga agcaacgttt cccaaatatt
     2161 cggttgccca ggggagaagt ggggctcttg gacaattccg gaggagttat ctatgcatat
     2221 aaggccctca gagccctgca ggatgcaatt cgacagctag gaggcatagt gcgtgacgga
     2281 gagaaggtgg tggagataaa cccagggcta ctggtcacgg tgaaaaccac ctccaggagc
     2341 taccaagcta agagcttggt catcacagca ggtccttgga ccaaccagct cctccgtccc
     2401 ctgggcattg agatgcctct ccagaccctg cggatcaacg tgtgttactg gcgagagatg
     2461 gttcctggga gctatggtgt gtcccaggcc tttccgtgct tcctgtggct gggcttgtgt
     2521 ccccaccaca tctacggact gcccacagga gagtacccag ggctgatgaa ggtcagctat
     2581 caccacggca accacgcaga ccctgaggag cgggactgcc ccacagcacg cacagacatc
     2641 ggagacgtcc agatcctgag cagctttgtc agagatcact tacctgatct gaagcccgag
     2701 cctgctgtca ttgagagctg catgtacacg aatacccctg atgagcagtt cattctcgat
     2761 cgccacccaa agtatgacaa cattgtcatt ggtgctggat tctctgggca cgggttcaag
     2821 ctggcccctg tggtggggaa gatcctgtat gaattaagca tgaaattaac accatcttat
     2881 gacttggcac cttttcgaat cagccgtttc ccaagcctgg gcaaagccca cctttgacac
     2941 ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag
     3001 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     3061 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     3121 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     3181 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     3241 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     3301 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     3361 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     3421 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     3481 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     3541 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     3601 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat
     3661 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3721 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3781 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3841 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3901 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3961 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4021 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4081 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     4141 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     4201 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat
     4261 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4321 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4381 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4441 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4501 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4561 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4621 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4681 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4741 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4801 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4861 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4921 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4981 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     5041 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     5101 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5161 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     5221 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     5281 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5341 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5401 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5461 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5521 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5581 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5641 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5701 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     5761 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5821 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5881 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5941 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6001 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6061 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6121 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6181 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6241 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6301 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6361 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6421 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6481 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6541 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6601 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6661 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6721 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6781 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6841 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6901 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6961 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7021 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7081 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7141 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7201 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7261 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7321 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
     7381 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7441 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7501 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7561 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7621 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7681 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     7741 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     7801 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     7861 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
     7921 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     7981 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     8041 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     8101 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     8161 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     8221 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     8281 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     8341 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8401 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8461 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     8521 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8581 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8641 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8701 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8761 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8821 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8881 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8941 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     9001 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     9061 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     9121 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9181 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     9241 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9301 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9361 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9421 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     9481 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     9541 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     9601 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     9661 acataacaaa ttggctgtg
//
