LOCUS Exported 2146 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly CD part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file. ACCESSION . VERSION . KEYWORDS C21m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2146) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2146) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2146 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 11..29 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 33..76 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 79..100 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 168..188 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(194..211) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(365..953) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(445..464) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1124..1984) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1747..1766 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1985..2089) /gene="bla" /label=AmpR promoter ORIGIN 1 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt 61 cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt ctcaaatggg 121 atcccgactg gcgagagcca ggtaacgaat ggatccaggt agagacctac tagtagcggc 181 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 361 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 781 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1261 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 1681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 1741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 1801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 1861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 1921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 1981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 2041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 2101 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacatt //