LOCUS Exported 2832 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).. ACCESSION . VERSION . KEYWORDS C35m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2832) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2832) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2832 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 14..32 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 36..79 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 82..103 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 857..877 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(883..900) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1054..1642) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1134..1153) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1813..2673) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2436..2455 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2674..2778) /gene="bla" /label=AmpR promoter ORIGIN 1 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc 61 tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagactagtg ggtctcaaat 121 gaggaatgac ggaggctttt tgctgtggtg ggacggtttg cgtagcgaga tgcagccgat 181 ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa gtgcggcgcc tgggcttcga 241 ttactacgcc tatggcgtgc gccacacgat tcccttcacc cggcccaaaa ccgaggtcca 301 tggcacctat cccaaggcct ggctggagcg ataccagatg cagaactacg gggccgtgga 361 tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg gtctggagcg acagcctgtt 421 cgaccagagc cggatgctct ggaacgaggc tcgcgattgg ggcctctgtg tcggcgcgac 481 cttgccgatc cgcgcgccga acaatttgct cagcgtgctt tccgtggcgc gcgaccagca 541 gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg ctgcgttgca tgatcgagtt 601 gctgacccag aagctgaccg acctggagca tccgatgctg atgtccaacc cggtctgcct 661 gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac ggcaagagtt ccggggaaat 721 cgccatcatc ctgagcatct ccgagagcac ggtgaacttc caccacaaga acatccagaa 781 gaagttcgac gcgccgaaca agacgctggc tgccgcctac gccgcggcgc tgggtttaat 841 ctaataggta gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct 901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 1021 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 1081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 1141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 1201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 1261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 1501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1921 accatctggc cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt 1981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 2041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 2101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 2161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 2221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 2281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 2341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 2401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 2461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 2521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 2581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2761 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 2821 tattatcatg ac //