LOCUS Exported 2146 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly BC part for MG-aptamer (fluorescent RNA aptamer of malachite green; see DOI: 10.1021/ja0529788) - Please see Supplemental Documents for annotated Genbank file. ACCESSION . VERSION . KEYWORDS U19m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2146) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2146) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2146 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 3..21 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 25..68 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 71..92 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" misc_feature 160..180 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(186..203) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(357..945) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(437..456) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1116..1976) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1739..1758 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1977..2081) /gene="bla" /label=AmpR promoter ORIGIN 1 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg 61 atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctcatact ggatcccgac 121 tggcgagagc caggtaacga atggatccaa tgagagacct actagtagcg gccgctgcag 181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 361 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 781 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgcgac 1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 1981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 2041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 2101 ccacctgacg tctaagaaac cattattatc atgacattaa cctata //