LOCUS Exported 2192 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).. ACCESSION . VERSION . KEYWORDS U6m SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2192) TITLE CIDAR MoClo Extension REFERENCE 2 (bases 1 to 2192) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2192 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 481..1069 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 970..989 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1223..1240 /label=L4440 /note="L4440 vector, forward primer" misc_feature 1246..1266 /label=BioBrick suffix /note="universal suffix for all parts" misc_feature 1380..1401 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 1404..1447 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(1451..1469) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1537..1641 /gene="bla" /label=AmpR promoter CDS 1642..310 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1860..1879) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 61 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 121 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtcgc gcggtatcat 181 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 241 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 301 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 601 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 721 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 781 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 841 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 901 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 961 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 1021 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctgtggaaaa acgccagcaa 1081 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 1141 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 1201 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca gcggccgcta 1261 ctagtaggtc tctcattaga aactttcctc agcatgatta agatgtttca gtacgaaaat 1321 tgctttcatt gttgatctcc tttttaagtg aacttgggcc cagtatgaga cccactagtc 1381 tctagaagcg gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat 1441 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 1501 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 1561 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 1621 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 1681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 1741 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 1801 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 1861 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 1921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 1981 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 2041 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 2101 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 2161 gacgagcgtg acaccacgat gcctgtagca at //