LOCUS       Exported                 594 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins 
            via tetrameric coiled-coil.
ACCESSION   .
VERSION     .
KEYWORDS    SpyCatcher002-oTet
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, 
            Howarth M
  TITLE     Spy
  JOURNAL   Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w.
  PUBMED    30988307
REFERENCE   2  (bases 1 to 594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt aaccacctta tcaggtttat caggtgagca aggtccgtcc
      121 ggtgatatga caactgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggccgtg agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acatgtgaag gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
      361 aatgagcaag gtcaggttac tgtaaatggc gaagcaacta aaggtgacgc tcatactgga
      421 tccagtggta gcggcagcgg ctctagtgac tattccgatt tacagcgcgt taaacaggaa
      481 ttgttggagg aggtcaagaa ggagttgcag aaggtcaaag aagaaatcat cgaggcattc
      541 gtgcaagagc tgcgtaaacg cggcagtggt tcgggcgaac cggaagcgta ataa
//
