LOCUS       Exported                 615 bp ds-DNA     linear   SYN 22-JUN-2021
DEFINITION  Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins
            via pentameric coiled-coil.
ACCESSION   .
VERSION     .
KEYWORDS    SpyCatcher002-oPent
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, 
            Howarth M
  TITLE     Spy
  JOURNAL   Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w.
  PUBMED    30988307
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggt aaccacctta tcaggtttat caggtgagca aggtccgtcc
      121 ggtgatatga caactgaaga agatagtgct acccatatta aattctcaaa acgtgatgag
      181 gacggccgtg agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt
      241 agtacatgga tttcagatgg acatgtgaag gatttctacc tgtatccagg aaaatataca
      301 tttgtcgaaa ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt
      361 aatgagcaag gtcaggttac tgtaaatggc gaagcaacta aaggtgacgc tcatactgga
      421 tccagtggta gcggcagcgg ctctatggac ttagcacccc aaatgttgcg cgaattgcaa
      481 gagaccaacg ctgccttgca ggatgtgcgc gagttgcttc gtcagcaagt taaggaaatc
      541 accttcttga agaatacggt catggagtgc gatgcgtgcg gaggcagtgg ttcgggcgaa
      601 ccggaagcgt aataa
//
