LOCUS       Exported                3189 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Human Invariant chain with a CLIP replacement with in frame 
            restriction sites (KpnI/XhoI).
ACCESSION   .
VERSION     .
KEYWORDS    pENTR-li-p33_RS-CLIP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Mensali N, Grenov A, Pati NB, Dillard P, Myhre MR, Gaudernack G, 
            Kvalheim G, Inderberg EM, Bakke O, Walchli S
  TITLE     Antigen-delivery through invariant chain (CD74) boosts CD8 and CD4 T
            cell immunity.
  JOURNAL   Oncoimmunology. 2019 Jan 11;8(3):1558663. doi: 
            10.1080/2162402X.2018.1558663. eCollection 2019.
  PUBMED    30723591
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(778..877)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(894..913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(895..913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(918..934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(918..934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1036..1055)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1047..1856
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1114..1133)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2003..2591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2492..2511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2745..2762
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2921..2948
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      3040..3126
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3175..8
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3189..17
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctccgcggcc gcccccttca ccatggatga ccagcgcgac cttatctcca
      181 acaatgagca actgcccatg ctgggccggc gccctggggc cccggagagc aagtgcagcc
      241 gcggagccct gtacacaggc ttttccatcc tggtgactct gctcctcgct ggccaggcca
      301 ccaccgccta cttcctgtac cagcagcagg gccggctgga caaactgaca gtcacctccc
      361 agaacctgca gctggagaac ctgcgcatga agcttcccaa gcctcccaag cctgtgagca
      421 agggtaccgc agcactcgag cagaatgcca ccaagtatgg caacatgaca gaggaccatg
      481 tgatgcacct gctccagaat gctgaccccc tgaaggtgta cccgccactg aaggggagct
      541 tcccggagaa cctgagacac cttaagaaca ccatggagac catagactgg aaggtctttg
      601 agagctggat gcaccattgg ctcctgtttg aaatgagcag gcactccttg gagcaaaagc
      661 ccactgacgc tccaccgaaa gagtcactgg aactggagga cccgtcttct gggctgggtg
      721 tgaccaagca ggatctgggc ccagtcccca tgtgaggatc caagggtggg cgcgccgacc
      781 cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
      841 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
      901 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
      961 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     1021 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     1081 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     1141 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     1201 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     1261 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
     1321 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     1381 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     1441 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     1501 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     1561 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     1621 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     1681 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     1741 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     1801 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     1861 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     1921 gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
     1981 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2041 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2101 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2161 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2221 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2281 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2341 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2401 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2461 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2521 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2581 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2641 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2701 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2761 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2821 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2881 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
     2941 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     3001 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     3061 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     3121 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     3181 cacgacgtt
//
