LOCUS       Exported                 720 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  mammalian expression of SPOON, a spontaneously blinking green 
            fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    SPOON/ pcDNA3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Arai Y, Takauchi H, Ogami Y, Fujiwara S, Nakano M, Matsuda T, Nagai 
            T
  TITLE     Spontaneously Blinking Fluorescent Protein for Simple Single Laser 
            Super-Resolution Live Cell Imaging.
  JOURNAL   ACS Chem Biol. 2018 Aug 17;13(8):1938-1943. doi: 
            10.1021/acschembio.8b00200. Epub 2018 Jul 10.
  PUBMED    29963852
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..717
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFVCTTGKLPVPWPSLLTTIGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNHDSHNVYITADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALGKDPNEKRDHMVLL
                     EFVTAAGITLGTDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt cgtctgcacc accggcaagc tgcccgtgcc ctggccctcc
      181 ctcctgacca ccatcggata cggcctgatg tgcttcgccc gctatcccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaaccacga cagccacaac gtctatatca cggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgggcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctactggagt tcgtgaccgc cgccgggatc accctcggca cggacgagct gtacaagtaa
//
