LOCUS       Exported               12448 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    RCAS GFP (CT#22)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12448)
  AUTHORS   Chen CM, Smith DM, Peters MA, Samson ME, Zitz J, Tabin CJ, Cepko CL
  TITLE     Production and design of more effective avian 
            replication-incompetent retroviral vectors.
  JOURNAL   Dev Biol. 1999 Oct 15. 214(2):370-84.
  PUBMED    10525341
REFERENCE   2  (bases 1 to 12448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12448
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(623..645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    634..650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(658..688)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(731..749)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        817..921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             922..1782
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1140..1159)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1953..2541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2442..2461
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2695..2712
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2727..2867
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2793..2812)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2953..2975
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2969..3160)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        4003..4264
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             11312..12031
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             11312..12028
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11357..11378)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11618..11637)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11965..11986
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        12220..33
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
       61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
      121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
      181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
      241 gagtaccgtc ggaggggatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
      301 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
      361 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
      421 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
      481 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
      541 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
      601 aaagctccaa gcttggctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat
      661 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gaattcttga
      721 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      781 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      841 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      901 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      961 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1021 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1081 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1141 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     1201 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1261 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1321 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1381 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1441 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     1501 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1561 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1621 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1681 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1741 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1801 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1861 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2341 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2581 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2641 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2701 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     2761 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     2821 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     2881 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     2941 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3001 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct
     3061 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     3121 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaaggag
     3181 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     3241 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     3301 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     3361 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     3421 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     3481 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     3541 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     3601 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg
     3661 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     3721 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     3781 ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc
     3841 ggggaggcag acaaggtata gggcggcgat gcgatgtacg ggccagatat acgcgtatct
     3901 gaggggacta gggtgtgttt aggcgaaaag cggggcttcg gttgtacgcg gttaggagtc
     3961 ccctcaggat atagtagttt cgcttttgca tagggagggg gaaatgtagt cttatgcaat
     4021 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     4081 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     4141 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttccgcattg cagagatatt
     4201 gtatttaagt gcctagctcg atacaataaa cgccatttga ccattcacca cattggtgtg
     4261 cacctgggtt gatggccgga ccgttgattc cctgacgact acgagcacct gcatgaagca
     4321 gaaggcttca tttggtgacc ccgacgtgat agttagggaa tagtggtcgg ccacagacgg
     4381 cgtggcgatc ctgtctccat ccgtctcgtc tatcgggagg cgagttcgat gaccctggtg
     4441 gagggggctg cggcttaggg aggcagaagc tgagtaccgt cggagggagc tccagggccc
     4501 ggagcgactg acccctgccg agaactcaga gggtcgtcgg aagacggaga gtgagcccga
     4561 cgaccacccc aggcacgtct ttggtcggcc tgcggatcaa gcatggaagc cgtcattaag
     4621 gtgatttcgt ccgcgtgtaa aacctattgc gggaaaatct ctccttctaa gaaggaaata
     4681 ggggccatgt tgtccctgtt acaaaaggaa gggttgctta tgtctccctc agatttatat
     4741 tctccggggt cctgggatcc catcactgcg gcgctctccc agcgggcaat ggtacttgga
     4801 aaatcgggag agttaaaaac ctggggattg gttttggggg cattgaaggc ggctcgagag
     4861 gaacaggtta catctgagca agcaaagttt tggttgggat tagggggagg gagggtctct
     4921 cccccaggtc cggagtgcat cgagaaacca gctacggagc ggcgaatcga caaaggggag
     4981 gaggtgggag aaacaactgt gcagcgagat gcgaagatgg cgccagagga agcggccaca
     5041 cctaaaaccg ttggcacatc ctgctatcat tgcggaacag ctgttggctg caattgcgcc
     5101 accgccacag cctcggcccc tcctccccct tatgtgggga gtggtttgta tccttccctg
     5161 gcgggggtgg gagagcagca gggccaggga gataacacgt ctcggggggc ggagcagcca
     5221 agggaggagc cagggcacgc gggtcaggcc cctgggccgg ccctgactga ctgggcaagg
     5281 gtaagggagg agcttgcgag tactggtccg cccgtggtgg ccatgcctgt agtgattaag
     5341 acagagggac ccgcctggac ccctctggag ccaaaattga tcacaagact ggctgatacg
     5401 gtcaggacca agggcttacg atccccgatc actatggcag aagtggaagc gctcatgtcc
     5461 tccccgttgc tgccgcatga cgtcacgaat ctaatgagag tgattttagg acctgcccca
     5521 tatgccttat ggatggacgc ttggggagtc caactccaga cggttatagc ggcagccact
     5581 cgcgaccccc gacacccagc gaacggtcaa gggcgggggg aacggactaa cttggatcga
     5641 ttaaagggct tagctgatgg gatggtgggc aacccacagg gtcaggccgc attattaaga
     5701 ccgggggaat tggttgctat tacggcgtcg gctctccagg cgtttagaga agttgcccgg
     5761 ctggcggaac ctgcaggtcc atgggcggac atcacgcagg gaccatctga gtcctttgtt
     5821 gattttgcca atcggcttat aaaggcggtt gaggggtcag atctcccgcc ttccgcgcgg
     5881 gctccggtga tcattgactg ctttaggcag aagtcacagc cagatattca gcagcttata
     5941 cgggcagcac cctccacgct gaccacccca ggagagataa tcaaatatgt gctagacagg
     6001 cagaagattg cccctcttac ggatcaaggc atagccgcgg ccatgtcgtc tgctatccag
     6061 cccttagtta tggcagtagt caatagagag agggatggac aaactgggtc gggtggtcgt
     6121 gcccgagggc tctgctacac ttgtggatcc ccgggacatt atcaggcaca gtgcccgaaa
     6181 aaacgaaagt caggaaacag ccgtgagcga tgtcagctgt gtgacgggat gggacacaac
     6241 gctaaacagt gtaggaagcg ggatggcaac cagggccaac gcccaggaag aggtctctct
     6301 tcggggccgt ggcccggccc tgagcagcct gccgtctcgt tagcgatgac aatggaacat
     6361 aaagatcgcc ccttggttag ggtcattctg actaacactg ggagtcatcc agtcaaacaa
     6421 cgttcggtgt atatcaccgc gctgttggac tccggagcgg acatcactat tatttcggag
     6481 gaggattggc ctactgattg gccggtggtg gacaccgcga acccacagat ccatggcata
     6541 ggagggggaa ttcccatgcg aaaatcccgg gatatgatag aggtgggggt tattaaccga
     6601 gacgggtcgt tggagcgacc cctgctcctc ttccccgcag tcgctatggt tagagggagt
     6661 atcctaggaa gagattgtct gcagggccta gggctccgct tgacaaattt atagggaggg
     6721 ccactgttct tactgttgcg ctacatctgg ctattccgct caaatggaag tcagaccgca
     6781 cgcctgtgtg gattgaccag tggccccttc ctgaaggtaa acttgtagcg ctaacgcaat
     6841 tagtggaaaa agaattacag ttaggacata tagagccctc acttagttgt tggaacacac
     6901 ctgtttttgt gatccggaag gcttccgggt cttatcgctt attgcatgat ttgcgcgctg
     6961 ttaacgccaa gcttgttcct tttggggccg tccaacaggg ggcgccagtt ctctccgcgc
     7021 tcccgcgtgg ctggcccctg atggtcctag acctcaagga ttgcttcttt tctatccctc
     7081 ttgcggaaca agatcgcgaa gcttttgcat ttacgctccc ctctgtgaat aaccaggccc
     7141 ccgctcgaag attccaatgg aaggtcttgc cccaagggat gacctgttct cccactatct
     7201 gtcagttggt agtgggtcag gtgctcgagc ccttgcgact caagcaccca gctctgcgca
     7261 tgttgcatta tatggacgat cttttgctag ccgcctcaag tcatgatggg ttggaagcgg
     7321 cagggaagga ggttatcggt acattggaaa gagccgggtt cactatttcg ccggataaga
     7381 tccagaggga gcccggagta caatatcttg ggtacaagtt aggcagtacg tatgtagcac
     7441 ccgtaggctt ggtagcagaa cccaggatag ccaccttgtg ggatgttcaa aagctggtgg
     7501 ggtcacttca gtggcttcgc ccagcgttag ggatcccgcc acgactgatg ggtccctttt
     7561 atgagcagtt acgagggtca gatcctaacg aggcgaggga atggaatcta gacatgaaaa
     7621 tggcctggag agagatcgta cagcttagca ctactgctgc cttggaacga tgggaccctg
     7681 cccagcctct ggaaggagcg gtcgctagat gtgaacaggg ggcaataggg gtcctgggac
     7741 agggactgtc cacacaccca aggccatgtt tgtggttatt ctccacccaa cccaccaagg
     7801 cgtttactgc ttggttagaa gtgctcaccc ttttgattac taagctacgc gcttcggcag
     7861 tgcgaacctt tggcaaggag gttgatatcc tcctgttgcc tgcatgcttc cgggaggacc
     7921 ttccgctccc ggaggggatc ctgttagcac ttagggggtt tgcaggaaaa atcaggagta
     7981 gtgacacgcc atctattttt gacattgcgc gtccactgca tgtttctctg aaagtgaggg
     8041 ttaccgacca ccctgtgccg ggacccactg tctttaccga cgcctcctca agcacccata
     8101 aaggggtggt agtctggagg gagggcccaa ggtgggagat aaaagaaata gttgatttgg
     8161 gggcaagtgt acaacaactg gaggcacgcg ctgtggccat ggcacttctg ctgtggccga
     8221 caacgcccac taatgtagtg actgactctg cgtttgttgc gaaaatgtta ctcaagatgg
     8281 gacaggaggg agtcccgtct acagcggcag cttttatttt agaggatgcg ttaagccaaa
     8341 ggtcagccat ggccgccgtt ctccacgtgc ggagtcattc tgaagtgcca gggtttttca
     8401 cagaaggaaa tgacgtggca gatagccaag ccacctttca agcgtatccc ttgagagagg
     8461 ctaaagatct tcataccgct ctccatattg gaccccgcgc gctatccaaa gcgtgtaata
     8521 tatctatgca gcaggctagg gaggttgttc agacctgccc gcattgtaat tcagcccctg
     8581 cgttggaggc cggggtaaac cctaggggtt tgggacccct acagatatgg cagacagact
     8641 ttacgcttga gcctagaatg gctccccgtt cctggctcgc tgttactgtg gacaccgcct
     8701 catcagcgat agtcgtaact cagcatggcc gtgttacatc ggttgctgca caacatcatt
     8761 gggccacggc tatcgccgtt ttgggaagac caaaggccat aaaaacagat aacgggtcct
     8821 gcttcacgtc taaatccacg cgggagtggc tcgcgagatg ggggatagca cacaccaccg
     8881 ggattccggg aaattcccag ggtcaagcta tggtagagcg ggccaaccgg ctcctgaaag
     8941 ataagatccg tgtgcttgcg gaaggggacg gctttatgaa aagaatcccc gccagcaaac
     9001 agggggaact actagccaaa gcaatgtatg ccctcaatca ctttgagcgt ggtgaaaaca
     9061 caaaaacacc ggtacaaaaa cactggagac ctaccgttct tacagaagga cccccggtta
     9121 aaatacgaat agagacaggg gagtgggaaa aaggatggaa cgtgctggtc tggggacgag
     9181 gttatgccgc tgtgaaaaac agggacactg ataaggttat ttgggtaccc tctcggaaag
     9241 ttaaaccgga tgtcacccaa aaggatgagg tgactaagaa agatgaggcg agccctcttt
     9301 ttgcaggcat ttctgactgg ataccctggg aagacgagca agaaggactc caaggagaaa
     9361 ccgctagcaa caagcaagaa agacccggag aagacaccct tgctgccaac gagagttaat
     9421 tatattctca ttattggtgt cctggtcttg tgtgaggtta cgggggtaag agctgatgtc
     9481 cacttactcg agcagccagg gaacctttgg attacatggg ccaaccgtac aggccaaacg
     9541 gatttttgcc tctctacaca gtcagccacc tccccttttc aaacatgttt gataggtatc
     9601 ccgtccccta tttccgaggg tgattttaag ggatatgttt ctgatacaaa ttgcaccacc
     9661 ttgggaactg atcggttagt ctcgtcagcc gactttactg gcggacctga caacagtacc
     9721 accctcactt atcggaaggt ctcatgcttg ttgttaaagc tgaatgtctc tatgtgggat
     9781 gagccacctg aactacagct gttaggttcc cagtctctcc ctaacattac taatattgct
     9841 cagatttccg gtataaccgg gggatgcgta ggcttcagac cacaaggggt tccttggtat
     9901 ctaggttggt ctagacagga ggccacgcgg tttctcctta gacacccctc tttctctaaa
     9961 tccacggaac cgtttacggt ggtgacagcg gataggcaca atctttttat ggggagtgag
    10021 tactgcggtg catatggcta cagattttgg aacatgtata actgctcaca ggtggggcgg
    10081 cagtaccgct gtggtaatgc gcgcagcccc cgcccgggtc ttcctgaaat ccagtgtaca
    10141 aggagaggag gcaaatgggt taatcaatca caggaaatta atgagtcgga gccgttcagc
    10201 tttacggtga actgtacagc tagtagtttg ggtaatgcca gtgggtgttg cggaaaagca
    10261 ggcacgattc tcccgggaaa gtgggtcgac agcacacaag gtagtttcac caaaccaaaa
    10321 gcgctaccac ccgcaatttt cctcatttgt ggggatcgcg catggcaagg aattcccagt
    10381 cgtccggtag ggggcccctg ctatttaggc aagcttacca tgttagcacc taagcataca
    10441 gatattctca aggtgcttgt caattcatcg cggacaggta taagacgtaa acgaagcacc
    10501 tcacacctgg atgatacatg ctcagatgaa gtgcagcttt ggggtcctac agcaagaatc
    10561 tttgcatcta tcctagcccc gggggtagca gctgcgcaag ccttaagaga aattgagaga
    10621 ctagcctgtt ggtccgttaa acaggctaac ttgacaacat cactcctcgg ggacttattg
    10681 gatgatgtca cgagtattcg acacgcggtc ctgcagaacc gagcggctat tgacttcttg
    10741 ctcctagctc acggccatgg ctgtgaggac gttgccggaa tgtgctgttt caatttgagt
    10801 gatcacagtg agtctataca gaagaagttc cagctaatga aggaacatgt caataagatc
    10861 ggcgtggaca gcgacccaat tggaagttgg ctgcgaggac tattcggggg aataggagaa
    10921 tgggccgttc atttgctgaa aggactgctt ttggggcttg tagttatttt gttgctagta
    10981 gtgtgcctgc cttgcctttt gcaaatcgta tgcggtaaca tcagaaagat gattaataac
    11041 tccatcagct accacacgga atataagaag ctgcaaaagg cctgtgggca gcctgaaagc
    11101 agaatagtat aaggcagtac atgggtggtg gtatagcgct tgcgagtcca tcgaggaagg
    11161 caggaaagac agctattggt aattgtgaaa tacgcttttg tctgtgtgct gcaggagctg
    11221 agctgactct gctggtggcc tcgcgtacca ctgtggcatc gattctagac cactgtggcc
    11281 aggcggtagc tgggacgtgc agccgaccac catggtgagc aagggcgagg agctgttcac
    11341 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
    11401 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
    11461 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
    11521 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
    11581 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
    11641 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
    11701 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
    11761 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
    11821 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
    11881 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
    11941 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
    12001 cactctcggc atggacgagc tgtacaagta aagcggccgc gactctaggg gatcctctag
    12061 agtcgacctg cagccaagct tatcgatgcg atgtacgggc cagatatacg cgtatctgag
    12121 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
    12181 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaatact
    12241 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
    12301 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
    12361 acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agatattgta
    12421 tttaagtgcc tagctcgata caataaac
//
