LOCUS       Exported                7715 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  mammalian expression of human androgen receptor with mutations of 
            N-terminal WxxLF motif.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hAR-W433A,L436A,F437A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   He B, Kemppainen JA, Wilson EM
  TITLE     FXXLF and WXXLF sequences mediate the NH2-terminal interaction with 
            the ligand binding domain of the androgen receptor.
  JOURNAL   J Biol Chem. 2000 Jul 28;275(30):22986-94.
  PUBMED    10816582
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3916..4538
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4115..4134)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4562..4582)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4567..4896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4747..4882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4809..4828
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4934..4953)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4935..4953)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4967..4983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4980..5002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4991..5007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5015..5045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5060..5081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5198..5215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5369..5957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5449..5468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6128..6988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6751..6770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6989..7093)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7120..7575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7207..7226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7417..7438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7701..8
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7715..17
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attcagatct gtccaccgtg tgtcttcttc tgcacgagac tttgaggctg tcagagcgct
      841 ttttgcgtgg ttgctcccgc aagtttcctt ctctggagct tcccgcaggt gggcagctag
      901 ctgcagcgac taccgcatca tcacagcctg ttgaactctt ctgagcaaga gaaggggagg
      961 cggggtaagg gaagtaggtg gaagattcag ccaagctcaa ggatggaagt gcagttaggg
     1021 ctgggaaggg tctaccctcg gccgccgtcc aagacctacc gaggagcttt ccagaatctg
     1081 ttccagagcg tgcgcgaagt gatccagaac ccgggcccca ggcacccaga ggccgcgagc
     1141 gcagcacctc ccggcgccag tttgctgctg ctgcagcagc agcagcagca gcagcagcag
     1201 cagcagcagc agcagcagca gcagcaagag actagcccca ggcagcagca gcagcagcag
     1261 ggtgaggatg gttctcccca agcccatcgt agaggcccca caggctacct ggtcctggat
     1321 gaggaacagc aaccttcaca gccgcagtcg gccctggagt gccaccccga gagaggttgc
     1381 gtcccagagc ctggagccgc cgtggccgcc agcaaggggc tgccgcagca gctgccagca
     1441 cctccggacg aggatgactc agctgcccca tccacgttgt ccctgctggg ccccactttc
     1501 cccggcttaa gcagctgctc cgctgacctt aaagacatcc tgagcgaggc cagcaccatg
     1561 caactccttc agcaacagca gcaggaagca gtatccgaag gcagcagcag cgggagagcg
     1621 agggaggcct cgggggctcc cacttcctcc aaggacaatt acttaggggg cacttcgacc
     1681 atttctgaca acgccaagga gttgtgtaag gcagtgtcgg tgtccatggg cctgggtgtg
     1741 gaggcgttgg agcatctgag tccaggggaa cagcttcggg gggattgcat gtacgcccca
     1801 cttttgggag ttccacccgc tgtgcgtccc actccttgtg ccccattggc cgaatgcaaa
     1861 ggttctctgc tagacgacag cgcaggcaag agcactgaag atactgctga gtattcccct
     1921 ttcaagggag gttacaccaa agggctagaa ggcgagagcc taggctgctc tggcagcgct
     1981 gcagcaggga gctccgggac acttgaactg ccgtctaccc tgtctctcta caagtccgga
     2041 gcactggacg aggcagctgc gtaccagagt cgcgactact acaactttcc actggctctg
     2101 gccggaccgc cgccccctcc gccgcctccc catccccacg ctcgcatcaa gctggagaac
     2161 ccgctggact acggcagcgc ctgggcggct gcggcggcgc agtgccgcta tggggacctg
     2221 gcgagcctgc atggcgcggg tgcagcggga cccggttctg ggtcaccctc agccgccgct
     2281 tcctcatccg cgcacactgc cgccacagcc gaagaaggcc agttgtatgg accgtgtggt
     2341 ggtggtgggg gtggtggcgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc
     2401 ggcggcgagg cgggagctgt agccccctac ggctacactc ggccccctca ggggctggcg
     2461 ggccaggaaa gcgacttcac cgcacctgat gtgtggtacc ctggcggcat ggtgagcaga
     2521 gtgccctatc ccagtcccac ttgtgtcaaa agcgaaatgg gcccctggat ggatagctac
     2581 tccggacctt acggggacat gcgtttggag actgccaggg accatgtttt gcccattgac
     2641 tattactttc caccccagaa gacctgcctg atctgtggag atgaagcttc tgggtgtcac
     2701 tatggagctc tcacatgtgg aagctgcaag gtcttcttca aaagagccgc tgaagggaaa
     2761 cagaagtacc tgtgcgccag cagaaatgat tgcactattg ataaattccg aaggaaaaat
     2821 tgtccatctt gtcgtcttcg gaaatgttat gaagcaggga tgactctggg agcccggaag
     2881 ctgaagaaac ttggtaatct gaaactacag gaggaaggag aggcttccag caccaccagc
     2941 cccactgagg agacaaccca gaagctgaca gtgtcacaca ttgaaggcta tgaatgtcag
     3001 cccatctttc tgaatgtcct ggaagccatt gagccaggtg tagtgtgtgc tggacacgac
     3061 aacaaccagc ccgactcctt tgcagccttg ctctctagcc tcaatgaact gggagagaga
     3121 cagcttgtac acgtggtcaa gtgggccaag gccttgcctg gcttccgcaa cttacacgtg
     3181 gacgaccaga tggctgtcat tcagtactcc tggatggggc tcatggtgtt tgccatgggc
     3241 tggcgatcct tcaccaatgt caactccagg atgctctact tcgcccctga tctggttttc
     3301 aatgagtacc gcatgcacaa gtcccggatg tacagccagt gtgtccgaat gaggcacctc
     3361 tctcaagagt ttggatggct ccaaatcacc ccccaggaat tcctgtgcat gaaagcactg
     3421 ctactcttca gcattattcc agtggatggg ctgaaaaatc aaaaattctt tgatgaactt
     3481 cgaatgaact acatcaagga actcgatcgt atcattgcat gcaaaagaaa aaatcccaca
     3541 tcctgctcaa gacgcttcta ccagctcacc aagctcctgg actccgtgca gcctattgcg
     3601 agagagctgc atcagttcac ttttgacctg ctaatcaagt cacacatggt gagcgtggac
     3661 tttccggaaa tgatggcaga gatcatctct gtgcaagtgc ccaagatcct ttctgggaaa
     3721 gtcaagccca tctatttcca cacccagtga agcattggaa accctatttc cccaccccag
     3781 ctcatgcccc ctttcagatg tcttctgcct gttataactc tgcactactc ctctgcagtg
     3841 ccttggggaa tttcctctat tgatgtacag tctgtcatga gcttgcatgc ctgcaggtcg
     3901 actctagagg atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     3961 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4021 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4081 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4141 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4201 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4261 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4321 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4381 ttccctgtcc ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat
     4441 aggctacctg gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc
     4501 tcatgcgttg ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg
     4561 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4621 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4681 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     4741 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     4801 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4861 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac
     4921 cagaaagtta attccctata gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc
     4981 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     5041 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     5101 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5161 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5221 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5281 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5341 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5401 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5461 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5521 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5581 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5641 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5701 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5761 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5821 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5881 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5941 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6001 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     6061 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6121 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6181 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6241 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6301 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6361 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6421 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6481 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6541 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6601 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6661 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6721 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6781 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6841 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6901 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6961 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     7021 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     7081 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     7141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     7381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7561 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     7621 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     7681 ttgggtaacg ccagggtttt cccagtcacg acgtt
//
