LOCUS       Exported               14248 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses disordered protein BRD4(462-1362) fused with fluorescent 
            protein miRFP670 and optogenetic protein Cry2WT.
ACCESSION   .
VERSION     .
KEYWORDS    pHR-BRD4-Delta-N-miRFP670-Cry2WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen
            NS, Haataja M, Brangwynne CP
  TITLE     Liquid Nuclear Condensates Mechanically Sense and Restructure the 
            Genome.
  JOURNAL   Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 
            10.1016/j.cell.2018.10.057.
  PUBMED    30500535
REFERENCE   2  (bases 1 to 14248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1604..1621
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3503..4447
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             4460..5953
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    6105..6693
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6158..6178)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6576..6587)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6696..6712)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6697..6713)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6803..7036
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7061..7079)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7062..7079)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7369..7388)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7602..7621)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7721..7743
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7781..7799)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7867..7971
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7972..8832
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8190..8209)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9003..9591
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9492..9511
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9745..9762
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9832..9852)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9837..10166
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10017..10152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10079..10098
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          11300..11365
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11495..11515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11940..12074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11977..11996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12031..12050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12242..12875
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12922..13047
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13544..13777
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13962..14006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgacagtgga tccggagcta ccatggagga
      781 gccagtggtg gccgtgtcct ccccggcagt gccccctccc accaaggttg tggccccgcc
      841 ctcatccagc gacagcagca gcgatagctc ctcggacagt gacagttcga ctgatgactc
      901 tgaggaggag cgagcccagc ggctggctga gctccaggag cagctcaaag ccgtgcacga
      961 gcagcttgca gccctctctc agccccagca gaacaaacca aagaaaaagg agaaagacaa
     1021 gaaggaaaag aaaaaagaaa agcacaaaag gaaagaggaa gtggaagaga ataaaaaaag
     1081 caaagccaag gaacctcctc ctaaaaagac gaagaaaaat aatagcagca acagcaatgt
     1141 gagcaagaag gagccagcgc ccatgaagag caagccccct cccacgtatg agtcggagga
     1201 agaggacaag tgcaagccta tgtcctatga ggagaagcgg cagctcagct tggacatcaa
     1261 caagctcccc ggcgagaagc tgggccgcgt ggtgcacatc atccagtcac gggagccctc
     1321 cctgaagaat tccaaccccg acgagattga aatcgacttt gagaccctga agccgtccac
     1381 actgcgtgag ctggagcgct atgtcacctc ctgtttgcgg aagaaaagga aacctcaagc
     1441 tgagaaagtt gatgtgattg ccggctcctc caagatgaag ggcttctcgt cctcagagtc
     1501 ggagagctcc agtgagtcca gctcctctga cagcgaagac tccgaaacag agatggctcc
     1561 gaagtcaaaa aagaaggggc accccgggag ggagcagaag aagcaccatc atcaccacca
     1621 tcagcagatg cagcaggccc cggctcctgt gccccagcag ccgcccccgc ctccccagca
     1681 gcccccrccg cctccacctc cgcagcagca acagcagccg ccacccccgc ctcccccacc
     1741 ctccatgccg cagcaggcag ccccggcgat gaagtcctcg cccccaccct tcattgccac
     1801 ccaggtgccc gtcctggagc cccagctccc aggcagcgtc tttgacccca tcggccactt
     1861 cacccagccc atcctgcacc tgccgcagcc tgagctgccc cctcacctgc cccagccgcc
     1921 tgagcacagc actccacccc atctcaacca gcacgcagtg gtctctcctc cagctttgca
     1981 caacgcacta ccccagcagc catcacggcc cagcaaccga gccgctgccc tgcctcccaa
     2041 gcccgcccgg cccccagccg tgtcaccagc cttgacccaa acacccctgc tcccacagcc
     2101 ccccatggcc caaccccccc aagtgctgct ggaggatgaa gagccacctg ccccacccct
     2161 cacctccatg cagatgcagc tgtacctgca gcagctgcag aaggtgcagc cccctacgcc
     2221 gctactccct tccgtgaagg tgcagtccca gcccccaccc cccctgccgc ccccacccca
     2281 cccctctgtg cagcagcagc tgcagcagca gccgccacca cccccaccac cccagcccca
     2341 gcctccaccc cagcagcagc atcagccccc tccacggccc gtgcacttgc agcccatgca
     2401 gttttccacc cacatccaac agcccccgcc accccagggc cagcagcccc cccatccgcc
     2461 cccaggccag cagccacccc cgccgcagcc tgccaagcct cagcaagtca tccagcacca
     2521 ccattcaccc cggcaccaca agtcggaccc ctactcaacc ggtcacctcc gcgaagcccc
     2581 ctccccgctt atgatacatt ccccccagat gtcacagttc cagagcctga cccaccagtc
     2641 tccaccccag caaaacgtcc agcctaagaa acaggagctg cgtgctgcct ccgtggtcca
     2701 gccccagccc ctcgtggtgg tgaaggagga gaagatccac tcacccatca tccgcagcga
     2761 gcccttcagc ccctcgctgc ggccggagcc ccccaagcac ccggagagca tcaaggcccc
     2821 cgtccacctg ccccagcggc cggaaatgaa gcctgtggat gtcgggaggc ctgtgatccg
     2881 gcccccagag cagaacgcac cgccaccagg ggcccctgac aaggacaaac agaaacagga
     2941 gccgaagact ccagttgcgc ccaaaaagga cctgaaaatc aagaacatgg gctcctgggc
     3001 cagcctagtg cagaagcatc cgaccacccc ctcctccaca gccaagtcat ccagcgacag
     3061 cttcgagcag ttccgccgcg ccgctcggga gaaagaggag cgtgagaagg ccctgaaggc
     3121 tcaggccgag cacgctgaga aggagaagga gcggctgcgg caggagcgca tgaggagccg
     3181 agaggacgag gatgcgctgg agcaggcccg gcgggcccat gaggaggcac gtcggcgcca
     3241 ggagcagcag cagcagcagc gccaggagca acagcagcag cagcaacagc aagcagctgc
     3301 ggtggctgcc gccgccaccc cacaggccca gagctcccag ccccagtcca tgctggacca
     3361 gcagagggag ttggcccgga agcgggagca ggagcgaaga cgccgggaag ccatggcagc
     3421 taccattgac atgaatttcc agagtgatct attgtcaata tttgaagaaa atcttttcgg
     3481 aggcggctcc ggaggcggaa gcatggtagc aggtcatgcc tctggcagcc ccgcattcgg
     3541 gaccgcctct cattcgaatt gcgaacatga agagatccac ctcgccggct cgatccagcc
     3601 gcatggcgcg cttctggtcg tcagcgaaca tgatcatcgc gtcatccagg ccagcgccaa
     3661 cgccgcggaa tttctgaatc tcggaagcgt actcggcgtt ccgctcgccg agatcgacgg
     3721 cgatctgttg atcaagatcc tgccgcatct cgatcccacc gccgaaggca tgccggtcgc
     3781 ggtgcgctgc cggatcggca atccctctac ggagtactgc ggtctgatgc atcggcctcc
     3841 ggaaggcggg ctgatcatcg aactcgaacg tgccggcccg tcgatcgatc tgtcaggcac
     3901 gctggcgccg gcgctggagc ggatccgcac ggcgggttca ctgcgcgcgc tgtgcgatga
     3961 caccgtgctg ctgtttcagc agtgcaccgg ctacgaccgg gtgatggtgt atcgtttcga
     4021 tgagcaaggc cacggcctgg tattctccga gtgccatgtg cctgggctcg aatcctattt
     4081 cggcaaccgc tatccgtcgt cgactgtccc gcagatggcg cggcagctgt acgtgcggca
     4141 gcgcgtccgc gtgctggtcg acgtcaccta tcagccggtg ccgctggagc cgcggctgtc
     4201 gccgctgacc gggcgcgatc tcgacatgtc gggctgcttc ctgcgctcga tgtcgccgtg
     4261 ccatctgcag ttcctgaagg acatgggcgt gcgcgccacc ctggcggtgt cgctggtggt
     4321 cggcggcaag ctgtggggcc tggttgtctg tcaccattat ctgccgcgct tcatccgttt
     4381 cgagctgcgg gcgatctgca aacggctcgc cgaaaggatc gcgacgcgga tcaccgcgct
     4441 tgagagcgcg gccacgcgta tgaagatgga caaaaagact atagtttggt ttagaagaga
     4501 cctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc
     4561 tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg
     4621 gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct
     4681 cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg
     4741 tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac
     4801 cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt
     4861 gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta
     4921 ctggaagaaa tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt
     4981 gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct
     5041 ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg
     5101 gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa
     5161 gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg
     5221 ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag
     5281 agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag
     5341 agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag
     5401 tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg
     5461 caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat
     5521 gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg
     5581 gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat
     5641 ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga
     5701 caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct
     5761 tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac
     5821 cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat
     5881 cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat
     5941 gatcggagca gcagcccggg atccaccggt cgccaccggt tcagggagcg gatccggctc
     6001 agggagtgcg gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta
     6061 gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt
     6121 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6181 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6241 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6301 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6361 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6421 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6481 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6541 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6601 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6661 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagggaatt
     6721 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6781 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6841 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7021 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     7081 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     7141 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     7201 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     7261 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     7321 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     7381 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     7441 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     7501 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     7561 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     7621 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7681 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7741 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7801 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7861 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7921 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7981 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8041 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8101 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8161 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8221 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8281 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8341 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8401 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8461 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8521 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8581 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8641 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8701 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8761 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8821 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8881 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8941 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9001 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9061 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9121 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9181 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9241 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9301 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9361 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9421 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9481 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9541 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9601 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9661 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9721 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     9781 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     9841 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9901 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9961 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    10021 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10081 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    10141 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
    10201 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
    10261 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
    10321 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
    10381 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
    10441 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
    10501 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
    10561 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
    10621 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
    10681 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
    10741 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
    10801 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
    10861 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
    10921 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
    10981 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
    11041 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
    11101 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
    11161 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
    11221 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
    11281 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
    11341 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
    11401 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
    11461 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
    11521 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
    11581 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
    11641 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
    11701 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
    11761 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
    11821 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
    11881 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
    11941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    12001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    12061 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    12121 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    12181 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    12241 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
    12301 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
    12361 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
    12421 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
    12481 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
    12541 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
    12601 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    12661 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
    12721 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    12781 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    12841 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    12901 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    12961 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    13021 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    13081 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    13141 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    13201 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    13261 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    13321 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    13381 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
    13441 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
    13501 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
    13561 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
    13621 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
    13681 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
    13741 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
    13801 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
    13861 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
    13921 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
    13981 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
    14041 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
    14101 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
    14161 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
    14221 acagagacag atccattcga ttagtgaa
//
